Black Seed Oil-induced Amelioration of Renal Dysfunction in a Rat Model of Diabetes Mellitus and Periodontitis


  • Osuvwe C. Orororo Department of Medical Biochemistry, Delta State University, Abraka, Delta State, Nigeria
  • Joseph C. Mordi Department of Medical Biochemistry, Delta State University, Abraka, Delta State, Nigeria
  • Ugbome A. Opute Department of Pharmacology, Therapeutics & Toxicology, College of Medicine, University of Lagos, Lagos State
  • Israel O. Efejene Deparment of Pharmacology, College of Medical and Health Sciences, Novena University, Ogume, Delta State, Nigeria
  • Egoamaka O. Egbune Department of Biochemistry, Delta State University, Abraka, Delta State, Nigeria
  • Abdulwasiu. A. Busari Department of Pharmacology, Therapeutics & Toxicology, College of Medicine, University of Lagos, Lagos State


Kidney, Nigella sativa, Periodontitis, Diabetes


This study explored the effects of administering black seed (Nigella sativa- NS) oil on the function and structure of the kidneys in rats with induced diabetes mellitus (DM) and/or periodontitis (PD). A total of 48 Wistar rats were separated into eight groups of six, with Group I serving as the control and Group II receiving NS oil along with their standard diet. Diabetes was induced in Group III, and NS oil was given to Group IV after diabetes had developed. PD was induced in Group V, and NS oil was administered after the disease was established in Group VI. Group VII had both DM and PD without treatment, while Group VIII received NS oil after the induction of DM and PD. Diabetes was induced using streptozotocin (STZ), while PD was induced using 3/0 silk sutures. Blood samples were collected through heart puncture, and the kidney was examined using histopathology. The levels of urea and creatinine were significantly increased (p<0.01) in the untreated diabetes mellitus and diabetes mellitus plus periodontitis groups compared to the untreated control group. Conversely, diabetes and periodontitis caused a significant (p<0.01) decrease in serum electrolyte levels compared to the control group. Both diabetes mellitus and periodontitis disrupted the normal kidney structure in the untreated group, but the administration of NS oil significantly reduced these effects in the treatment groups. The study's biochemical and histological findings indicate the potential beneficial effects of Nigella sativaoil and its bioactive components in combating kidney damage caused by diabetes and periodontitis. 


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How to Cite

Orororo, O. C., Mordi, J. C., Opute, U. A., Efejene, I. O., Egbune, E. O., & Busari, A. A. (2023). Black Seed Oil-induced Amelioration of Renal Dysfunction in a Rat Model of Diabetes Mellitus and Periodontitis: Tropical Journal of Natural Product Research (TJNPR), 7(7), 3524–3531. Retrieved from