In-vitro and In-silico Studies of a Phenylpropanoid Compound Isolated from Sterculia quadrifida Seeds and Its Inhibitory Effect on Matrix Metalloproteinase-9

http://www.doi.org/10.26538/tjnpr/v7i7.30

Authors

  • Rollando Rollando Pharmacy Department, Faculty of Health Sciences, Ma Chung University, Malang 65151, Indonesia
  • Eva Monica Pharmacy Department, Faculty of Health Sciences, Ma Chung University, Malang 65151, Indonesia
  • Muhammad H. Afthoni Pharmacy Department, Faculty of Health Sciences, Ma Chung University, Malang 65151, Indonesia
  • Warsito Warsito Chemistry Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, Indonesia
  • Masruri Masruri Chemistry Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, Indonesia
  • Nashi Widodo Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, Indonesia
  • Rahadian Zainul Chemistry Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang 25131, Indonesia

Keywords:

Sterculia quadrifida, MMP-9, breast cancer, 5-diphenylpenta-2,4-dien-1-one, (2E,4E)-1

Abstract

Previous investigation discovered a phenylpropanoid compound called (2E,4E)-1,5-diphenylpenta-2,4-dien-1-one in the chloroform fraction of Sterculia quadrifida seeds. This  compound exhibited notable cytotoxic effects, displaying IC50 values of 2.29, 9.93, 18.09, and 12.12 µg/mL in 4T1, MCF-7, MDA-MB-435, and T47D breast cancer cell lines respectively. In our current study, we performed an MMP-9 enzyme inhibition test using the fluorescence resonance energy transfer (FRET) assay and utilized molecular docking and molecular dynamics simulations to explore the compound's mechanism of action. The results revealed that (2E,4E)- 1,5-diphenylpenta-2,4-dien-1-one effectively inhibited the MMP-9 enzyme, achieving a level of 95.78% inhibition with an IC50 value of 34.78 µg/mL. Furthermore, the molecular docking and dynamics simulations indicated robust and stable interactions between (2E,4E)-1,5-diphenylpenta-2,4-dien-1-one and the enzyme's catalytic site. In summary, this study offers significant insights into the development of herbal medicines with promising potential as anticancer drugs. 

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Published

2023-07-31

How to Cite

Rollando, R., Monica, E., Afthoni, M. H., Warsito, W., Masruri, M., Widodo, N., & Zainul, R. (2023). In-vitro and In-silico Studies of a Phenylpropanoid Compound Isolated from Sterculia quadrifida Seeds and Its Inhibitory Effect on Matrix Metalloproteinase-9: http://www.doi.org/10.26538/tjnpr/v7i7.30. Tropical Journal of Natural Product Research (TJNPR), 7(7), 3490–3495. Retrieved from https://tjnpr.org/index.php/home/article/view/2251

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