HPLC Analysis and Protective Effect of Fractions from Ethanolic Extract of Calotropis procera (Ait.) R. Br Root Bark Against Diethylnitrosamine Induced-Hepatic Damage in Wistar Rats

http://www.doi.org/10.26538/tjnpr/v7i7.26

Authors

  • Ernest N. Sombié Laboratory of Biochemistry and Applied Chemistry, Department of Biochemistry-Microbiology, Faculty of Science and Technology, Joseph Ki-Zerbo University, Ouagadougou, Burkina Faso
  • Donzèo G. Somda Drug Development Laboratory, Department of Pharmacology and Toxicology, Faculty of Health Sciences, Joseph Ki-Zerbo University, Ouagadougou, Burkina Faso
  • Soumaila Konaté National Agency for Environmental, Food, Occupational and Health Product Safety, Ouagadougou, Burkina Faso
  • Ollo Youl Laboratory of Biochemistry and Applied Chemistry, Department of Biochemistry-Microbiology, Faculty of Science and Technology, Joseph Ki-Zerbo University, Ouagadougou, Burkina Faso
  • Tata K. Traoré Drug Development Laboratory, Department of Pharmacology and Toxicology, Faculty of Health Sciences, Joseph Ki-Zerbo University, Ouagadougou, Burkina Faso
  • Jotham Y-P. N’do Laboratory of Biochemistry and Applied Chemistry, Department of Biochemistry-Microbiology, Faculty of Science and Technology, Joseph Ki-Zerbo University, Ouagadougou, Burkina Faso
  • Wendkouni L.M.E. Belem-Kabré Laboratory of Biochemistry and Applied Chemistry, Department of Biochemistry-Microbiology, Faculty of Science and Technology, Joseph Ki-Zerbo University, Ouagadougou, Burkina Faso
  • Geoffroy G. Ouédraogo epartment of Medicine and Traditional Pharmacopoeia/Pharmacy, Institute for Research in Health Sciences, National Center for Scientific and Technological Research, Ouagadougou Burkina Faso
  • Noufou Ouédraogo Department of Medicine and Traditional Pharmacopoeia/Pharmacy, Institute for Research in Health Sciences, National Center for Scientific and Technological Research, Ouagadougou Burkina Faso
  • Adama Hilou Laboratory of Biochemistry and Applied Chemistry, Department of Biochemistry-Microbiology, Faculty of Science and Technology, Joseph Ki-Zerbo University, Ouagadougou, Burkina Faso

Keywords:

anti-hepatotoxic activities, phytochemical molecules, diethylnitrosamine, Calotropis procera, Liver Diseases

Abstract

Calotropis procera (Ait.) R.Br (Apocynaceae) is a medicinal plant known for its multiple therapeutic virtues including the management of liver diseases in Burkina Faso. This study aimed to evaluate the hepato-protective properties of the fractions of the ethanol extract of Calotropis procera roots barks while identifying chemical compounds in these extracts. Sterols, triterpene and phenolic compounds were sought and quantified in the fractions of the ethanol extract of Calotropis procera root bark by HPLC-DAD. The anti-hepatotoxic activity of fractions was evaluated using diethylnitrosamine (DEN) to induce acute liver inflammation in male Wistar rats. Serum biomarkers, alanine transaminase (ALAT), aspartate transaminase (ASAT), alkaline phosphatase (ALP), total proteins and Albumin were evaluated and the activities of antioxidant enzymes (Superoxide dismutase and catalase) as well as the level of malondialdehyde (MDA) were determined in the liver homogenate. The histopathological study of sections of livers pretreated with the fractions was also carried out. The evaluation of the anti-hepatotoxic activity showed that the n-butanol fraction and residual aqueous fraction had best effects on serum biomarkers (ALAT, ASAT, ALP) as well as on the activities of the antioxidant enzymes Superoxide dismutase and catalase and on hepatic tissue. Gallic acid, an antioxidant, antihepatotoxic, antifibrotic compound was identified in significant quantities in these two fractions at the respective contents of 17.92 ± 0.26 μg/mg and 18.76 ± 0.27 μg/mg of dry extract, suggesting
that this compound would participates in the hepatoprotective properties of these extracts. 

Author Biographies

Ollo Youl, Laboratory of Biochemistry and Applied Chemistry, Department of Biochemistry-Microbiology, Faculty of Science and Technology, Joseph Ki-Zerbo University, Ouagadougou, Burkina Faso

National Agency for Environmental, Food, Occupational and Health Product Safety, Ouagadougou, Burkina Faso

Noufou Ouédraogo, Department of Medicine and Traditional Pharmacopoeia/Pharmacy, Institute for Research in Health Sciences, National Center for Scientific and Technological Research, Ouagadougou Burkina Faso

Drug Development Laboratory, Department of Pharmacology and Toxicology, Faculty of Health Sciences, Joseph Ki-Zerbo University, Ouagadougou, Burkina Faso

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Published

2023-07-31

How to Cite

Sombié, E. N., Somda, D. G., Konaté, S., Youl, O., Traoré, T. K., N’do, J. Y.-P., … Hilou, A. (2023). HPLC Analysis and Protective Effect of Fractions from Ethanolic Extract of Calotropis procera (Ait.) R. Br Root Bark Against Diethylnitrosamine Induced-Hepatic Damage in Wistar Rats: http://www.doi.org/10.26538/tjnpr/v7i7.26. Tropical Journal of Natural Product Research (TJNPR), 7(7), 3462–3469. Retrieved from https://tjnpr.org/index.php/home/article/view/2247