Evaluation of the Antioxidant, Antifungal and Anticholinesterase activity of the Extracts of Ruta Montana L., Harvested from Souk-Ahras (North-East of Algeria) and Composition of its Extracts by GC-MS



  • Nabila Slougui BioGeoChemistry of Desert Environments Laboratory, Kasdi Merbah Ouargla University, Ouargla (30000), Algeria
  • Nada L Rabhi National Polytechnic School of Constantine - Ali Mendjeli University City - BP 75A RP Ali Mendjeli – Constantine (25016), Algeria.
  • Rahil Achouri National Polytechnic School of Constantine - Ali Mendjeli University City - BP 75A RP Ali Mendjeli – Constantine (25016), Algeria.
  • Chawki Bensouici Biotechnology Research Center (BTRC), Constantine (25016), Algeria


GC-MS, essential oil, anti-cholinesterase activity, antifungal activity, antioxidant activity, Ruta montana


Ruta montana is a wild medicinal plant traditionally used by ancient populations to treat various diseases The aimed is to evaluate the antioxidant, antifungal and anti-cholinesterase activities of six extracts [Essential oil (01), Soxhlet extract (02), the hexane extract by maceration (03), the extract Chloroform (04), the extract with ethyl acetate (05) and the butanol extract (06)], obtained from the aerial part of Ruta montana L., harvested from Souk-Ahras North-East from Algeria. For the antioxidant activity, seven tests were used. The results, all tests included, confirm that the extracts of Soxhlet (02), chloroform (04), ethyl acetate (05) and butanol (06) have the best
antioxidant activity with IC50 = 173.54±7.27 μg/ml, A0.5= 46.21±3.99 μg/ml, A0.5= 35.06±0.49 μg/ml and IC50= 59.48±7.79 μg/ml respectively. For the evaluation of the antifungal activity, it was found that the chloroform extract (04) gave better antifungal activity against the fungus (Fusarium oxysporum f. sp. lycopersici (FOL)) with a fungistatic effect which is translated to an inhibition rate of 11.91% at 1mg and 60.91% at 40mg. The composition of essential oil (01), of Soxhlet extract (02) and hexane extract (03) was evaluated by GC-MS with a major composition of 2-nonanone (24.93%), 2- undecanone (22.62%) and Acetic acid, non-2-yl ester (14.15%), for
the essential oil; hydrocarbons (56.89%), 2-undecanone (10.53%), 2-nonanone (9.24%) and the alkaloid Dictamnine,6,7-dimethoxy with a rate of (8.39%), for soxhlet extract (02) and for hexane extract (03) the hydrocarbons are present with a rate of (47.19%) followed by 2-nonanone (13.31%) and 2-undecanone with a rate of 8.44%. 

Author Biography

Nabila Slougui, BioGeoChemistry of Desert Environments Laboratory, Kasdi Merbah Ouargla University, Ouargla (30000), Algeria

National Polytechnic School of Constantine - Ali Mendjeli University City - BP 75A RP Ali Mendjeli – Constantine (25016), Algeria.


Odion EE, Falodun A and Adelusi SA. Total flavonoid, Total Phenolic and antioxidant potential of root bark extract and fractions of from Cola rostrata (Sterculiaceae) K. Schum. University of Benin 2013; J. Sci. Tech 1 (2): 38 - 42.

Ngozi P Okolie, Falodun A and Oluseyi Davids. Evaluation of the antioxidant activity of root extract of pepper fruit (Dennetia tripetala), and its potential for the inhibition of Lipid peroxidation. Afri J Trad Complem and Altern Med 2014; 11(3):221-227.

Egharevba E, Chukwuemeke-Nwani P, Eboh U, Okoye E, Bolanle IO, Oseghale IO, Imieje VO, Erharuyi O, Falodun A. Antioxidant and Hypoglycaemic Potentials of the Leaf Extracts of Stachytarphyta jamaicensis (Verbenaceae). Trop J Nat Prod Res. 2019; 3(5):170-174.

Narayanaswamy N, Balakrishnan K. Evaluation of some Medicinal Plants for their Antioxidant Properties. Int. J. Pharmtech Res. 2011;3(1):381-385.

Ben Sghaier M, Louhichi T , Hakem A, Ammari Y. Chemical investigation of polar extracts from Ruta chalpensis L., growing in Tunisia: Correlation with their antioxidant activities. Journal of new sciences. 2018; 49(4): 2971-2978

Khlifi D, Sghaier RM, Amouri S, Laouini D, Hamdi M, Bouajila J. Composition and antioxidant,anti-cancer and anti-inflammatory activities of Artemisia herba-alba, Ruta chalpensis L.and Peganum harmala L. Food Chem Toxicol. 2013; 55:202–208.

Poonkodi K, Gomathi K, Akila M, Deepadevi S, Dhivya A. Gas chromatography-mass spectrometry analysis and in vitro antioxidant activities of Ruta graveolens L., from Western Ghats region - South India. Asian J Pharm Clin Res. 2017; 10(5): 297–301.


Mejri J, Abderrabba M, Mejri M. Chemical composition of the essential oil of Ruta chalepensis L., Influence of drying, hydro-distillation duration and plant parts. Ind Crops Prod. 2010; 32 :671–673

Touati D, Atta-ur-Rahman, Ulubelen A. Alkaloids from Ruta montana. Phytochem. 2000; 53(2): 277-279, https://doi.org/10.1016/S0031-9422(99)00486-0.

Kabouche Z, Benkiki N, Seguin E, Bruneau C. A new dicoumarinyl ether and two rare furocoumarins from Ruta montana. Fitoterapia. 2003;74(1-2):194-196. doi: 10.1016/s0367-326x(02)00313-1. PMID: 12628424.

Eun-Tae O, Jiho L, Chang-Soo K, Young-Soo K. Alkylquinolone alkaloid profiles in Ruta graveolens. Biochem Syst Ecol. 2014;. 57: 384-387.


Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N. Antioxidant activity of some algerian medicinal plants extracts containing phenolic compounds, Food Chem. 2006; 97(4):654-660, https://doi.org/10.1016/j.foodchem.2005.04.028.

Kambouche N, Merah B, Bellahouel S, Bouayed J, Dicko A, Derdour A, Younos C, Soulimani R. Chemical composition and antioxidant potential of Ruta montana L., essential oil from Algeria. J Med Food. 2008;11(3):593-595. doi: 10.1089/jmf.2007.0515. PMID: 18800912.

Bouzidi MA, Latrèche A, Attaoui I, Benabderrahmane M, Mehdadi Z, Benyahia M. Antibacterial Effect of the Essential Oils Extracted From Ruta chalepensis L., and Ruta montana (L.) L. J Life Sci. 2012; 6: 898-902

Djarri L, Ferhat M & Merabet G, Chelghoum, Laggoune S, Semra Z. Smati F, Kabouche Z. Composition and antibacterial activity of the essential oil of Ruta montana from Constantine (Algeria). Der Pharm Lett. 2013; 5: 70-73.

Hammami I, Smaoui S, Hsouna A B, Hamdi N, Triki M A. Ruta montana L., leaf essential oil and extracts: characterization of bioactive compounds and suppression of crown gall disease. EXCLI J. 2015; 14: 83–94. https://doi.org/10.17179/excli2014-655

Farid O, Hebi M, Ajebli M, Hidani AE, Eddouks M. Antidiabetic effect of Ruta montana L. in streptozotocininduced diabetic rats. J Basic Clin Physiol Pharmacol. 2017; 28(3): 275-282. doi: 10.1515/jbcpp-2016-0030. PMID: 28121617.

Khadhri A, Bouali I, Belkhir S, Mokded R, Smiti S, Falé P, Araújo M E M, Serralheiro M L M. In vitro digestion, antioxidant and antiacetylcholinesterase activities of two species of Ruta: Ruta chalepensis and Ruta montana. Pharm. Biol. 2017; 55(1): 101-107, DOI:


Fekhar N, Moulay S, Drioueche A, Krea Mohamed, Boutoumi H, Benmaamar, Z. Thionation of Essential Oils from Algerian Artemisia Herba-alba L. and Ruta Montana L.: Impact on their Antimicrobial and Insecticidal Activities. Chem J Mold. 2017; 12(2): 50-57. 10.19261/cjm.2017.410.

Amarti F, Satrani B, Ghanmi M, Farah A, Aafi A, Arab L, El Ajjouri M, Chaouch A. Composition chimique et activité antimicrobienne des huilles essentielles de Thymus algeriensis Boiss. & Reut. et Thymus ciliatus (Dest.) Benth. du Maroc. Biotechnol. Agron. Environ. 2010; 14(1): 141 - 148.

Blois M S. Antioxydant determinations by the use of a stable free radical. Nature. 1958., 4617(181):1119-1200.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, RiceEvans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999; 26 (9-10): 1231-1237. doi:10.1016/s0891- 5849(98)00315-3. PMID: 10381194.

Shi H, Noguchi N, Niki E. Galvinoxyl method for standardizing electron and proton donation activity. Methods Enzymol. 2001; 335:157-66. doi: 10.1016/s0076- 6879(01)35240-0. PMID: 11400365.

Makoto O. Studies on products of browning reaction. Antioxidative activities of products of browning reaction prepared from glucosamine.. Jpn J Nutr Diet. 1986; 44(6): 307-315. DOI: 10.5264/eiyogakuzashi.44.307

Szydłowska-Czerniak A, Dianoczki C, Recseg K, Karlovits G, Szłyk E. Determination of antioxidant capacities of vegetable oils by ferric-ion spectrophotometric methods. Talanta. 2008; 15: 76(4):899-905. doi: 10.1016/j.talanta.2008.04.055. PMID: 18656676.

Ozyürek M, Güngör N, Baki S, Güçlü K, Apak R. Development of a silver nanoparticle-based method for the antioxidant capacity measurement of polyphenols. Anal Chem. 2012; 84(18): 8052-9. doi: 10.1021/ac301925b. PMID: 22897622.

Apak R, Güçlü K, Ozyürek M, Karademir SE. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J Agric Food Chem. 2004;52(26): 7970-81. doi: 10.1021/jf048741x. PMID: 15612784.

Ellman G L, Courtney K D, Andres V, Featherstone R M. A new and rapid colorimetric determination of acetylcholinesterase activity, Biochem Pharmacol. 1961; 7(2) : 88-95. https://doi.org/10.1016/0006-2952(61)90145-9.

Soro D, Kone M W, Kone D, Kamanzi K. Evaluation De L’activité Antifongique Par Bioautographie De Quelques Plantes Medicinales De Côte D’ivoire Contre Deux Formes Speciales De Fusarium oxysporum. Agron Afr. 2012 ;. 24 (1) : 19 - 28

Weitang S, Ligang Z, Chengzong Y, Xiaodong C, Li-Qun Z, Xili L. Tomato Fusarium wilt and its chemical control strategies in a hydroponic system. Crop Prot. 2004; 23: 243- 247. 10.1016/j.cropro.2003.08.007.

Dennis C E, Webster J. Antagonistic properties of speciesgroups of Trichoderma III. Hyphal interaction. Trans. Br. Mycol. Soc. 1971; 57:363-369. 10.1016/S0007- 1536(71)80050-5.

ADAMS R P. Identification of Essential Oil Components By Gas Chromatography/Mass Spectrometry (4th ed.). Allured Pub Corp; 2007. 804p

Mohammedi H, Mecherara-Idjeri S & Hassani A. Variability in essential oil composition, antioxidant and antimicrobial activities of Ruta montana L. collected from different geographical regions in Algeria. J Essent Oil Res. 2020; 32(1): 88-101. DOI: 10.1080/10412905.2019.1660238

Zellagui A, Belkassam A, Belaidi A, Gherraf N. Environmental impact on the Chemical Composition and yield of essential oils of Algerian Ruta Montana (Clus.) L and their antioxidant and antibacterial activities. Adv Environ Biol. 2012; 6(10): 2684-2688.

Poonkodi K, Gomathi K, Akila M, Deepadevi S, Dhivya A. Gas Chromatography-Mass Spectrometry Analysis And In Vitro Antioxidant Activities of Ruta Graveolens L. From Western Ghats Region - South India. Asian J Pharm Clin Res. 2017; 10 (05): 297-301.

Ouerghemmi I, Bettaieb Rebey I, Rahali F Z, Bourgou S, Pistelli L, Ksouri R, Marzouk B, Saidani Tounsi M. Antioxidant and antimicrobial phenolic compounds from extracts of cultivated and wild-grown Tunisian Ruta chalepensis. J Food Drug Anal. 2017; 25(2): 350-359. doi:

1016/j.jfda.2016.04.001.. PMID: 28911677.

Drioiche A, Amine S, Boutahiri S, Saidi S, Ailli A, Rhafouri R, Mahjoubi M, El Hilali F, Mouradi A, Eto B, Zair T. Antioxidant and Antimicrobial Activity of Essential Oils and Phenolic Extracts from the Aerial Parts of Ruta montana L., of the Middle Atlas Mountains-Morocco. J Essent Oil-Bear. 2020; 23(5): 902-917, DOI: 10.1080/0972060X.2020.1829995

Coimbra AT, Ferreira S, Duarte AP. Genus Ruta: A natural source of high value products with biological and pharmacological properties. J Ethnopharmacol. 2020; 260: 113076. doi: 10.1016/j.jep.2020.113076. Epub 2020 Jun 10. PMID: 32534112.

Merghem M, Dahamna S. In-Vitro Antioxidant Activity and Total Phenolic Content of Ruta montana L. Extracts. J Drug Deliv Ther. 2020; 10: 69-75. 10.22270/jddt.v10i2.3919.

Benali T, Habbadi K, Khabbach A, Marmouzi I, Zengin G, Bouyahya A, Chamkhi I, Chtibi H, Aanniz T, Achbani EH, Hammani K. GC-MS Analysis, Antioxidant and Antimicrobial Activities of Achillea Odorata Subsp. Pectinata and Ruta Montana Essential Oils and Their Potential Use as Food Preservatives. Foods. 2020; 9(5): 668. doi: 10.3390/foods9050668. PMID: 32455872; PMCID: PMC7278837.

Merghem M, Dahamna S, Khennouf S. In Vivo Antioxidant Activity of Ruta montana L. Extracts. J. Mater. Environ. Sci. 2019; 10 (5): 470-477.

Bennaoum Z, Benhassaini H, Falconieri D, Piras A, Porcedda S. Chemical variability in essential oils from Ruta species among seasons, and its taxonomic and ecological significance. Nat. Prod. Res. 2017; 31(19): 2329-2334, DOI: 10.1080/14786419.2017.1303692

Barbouchi M, Benzidia B, Choukrad M. Chemical variability in essential oils isolated from roots, stems, leaves and flowers of three Ruta species growing in Morocco. J King Saud Univ Sci. 2021;.33(8): 101634. 10.1016/j.jksus.2021.101634



How to Cite

Slougui, N., Rabhi, N. L., Achouri, R., & Bensouici, C. (2023). Evaluation of the Antioxidant, Antifungal and Anticholinesterase activity of the Extracts of Ruta Montana L., Harvested from Souk-Ahras (North-East of Algeria) and Composition of its Extracts by GC-MS: http://www.doi.org/10.26538/tjnpr/v7i7.25. Tropical Journal of Natural Product Research (TJNPR), 7(7), 3452–3461. Retrieved from https://tjnpr.org/index.php/home/article/view/2246