Antioxidant Activities of Enriched Polysaccharide Fractions from Mycelia of Amauroderma subresinosum

http://www.doi.org/10.26538/tjnpr/v7i7.24

Authors

  • Trang T.T. Nguyen Faculty of Biology and Biotechnology, University of Science, 227 Nguyen Van Cu, District 5, Ho Chi Minh City 749000, Vietnam
  • Ngoc A.T. Pham Faculty of Biology and Biotechnology, University of Science, 227 Nguyen Van Cu, District 5, Ho Chi Minh City 749000, Vietnam
  • Quoc T. Tran Faculty of Biology and Biotechnology, University of Science, 227 Nguyen Van Cu, District 5, Ho Chi Minh City 749000, Vietnam
  • Huy D. Nguyen Vietnam National University, Linh Trung, Thu Duc, Ho Chi Minh city 721400, Vietnam
  • Duc H. Nguyen Faculty of Biology and Biotechnology, University of Science, 227 Nguyen Van Cu, District 5, Ho Chi Minh City 749000, Vietnam
  • Khanh H.T. Pham Faculty of Biology and Biotechnology, University of Science, 227 Nguyen Van Cu, District 5, Ho Chi Minh City 749000, Vietnam
  • Manh H. Tran School of Medicine and Pharmacy, The University of Danang, Hoa Quy, Ngu Hanh Son district, Danang 550000, Vietnam
  • Linh T. Tran Faculty of Biology and Biotechnology, University of Science, 227 Nguyen Van Cu, District 5, Ho Chi Minh City 749000, Vietnam

Keywords:

ABTS, DPPH, mycelia, polysaccharide fractions, Ganodermataceae, Amauroderma subresinosum

Abstract

The polysaccharides found in Amauroderma subresinosum have been recognized, but their characterization has been limited. In this study, enriched polysaccharide fractions (ASF) were extracted from the mycelia of Amauroderma subresinosum using hot water, sodium hydroxide, and acetone. To determine these ASF structural characteristics, deproteination and precipitation with cold ethanol were performed to remove residual proteins. Fourier transform infrared (FTIR) spectroscopy was employed to assess the purity of the ASF, while gel permeation chromatography (GPC) was used to measure molecular weight, and high-performance liquid chromatography with precolumn derivatization using 1-phenyl-3-methyl-5-pyrazolone (PMP) reagent was utilized for monosaccharide analysis. The antioxidant activity of the ASF was evaluated through DPPH and ABTS radical scavenging assays. Seven enriched  olysaccharide fractions, named ASF1–7, were isolated, and three main fractions were identified as ASF-1 (22.53 kDa, 6.16 ± 1.01%, w/w), ASF- 3 (0.75 kDa, 16.46 ± 0.26%, w/w), and ASF-7 (35.73 kDa, 12.60 ± 1.48%, w/w). These fractions corresponded to the primary polysaccharide derived from hot water, alkaline solution, and insoluble residue. Monosaccharide analysis revealed that glucose was the predominant component in ASF-1 (70.24%), ASF-3 (84.11%), and ASF-7 (87.78%). FTIR spectroscopy confirmed the presence of carboxyl and hydroxyl groups, as well as pyranose rings in these ASFs. Additionally, ASF-1, ASF-3, and ASF-7 demonstrated significant  PPH and ABTS radical scavenging activities, with IC50 values ranging from 12.8 to 16.1 µg/mL and 8.5 to 12.6 µg/mL, respectively.This study provides the initial evidence that polysaccharides extracted from Amauroderma subresinosum may possess potential as functional food ingredients with potent antioxidant activity. 

Author Biographies

Trang T.T. Nguyen, Faculty of Biology and Biotechnology, University of Science, 227 Nguyen Van Cu, District 5, Ho Chi Minh City 749000, Vietnam

Vietnam National University, Linh Trung, Thu Duc, Ho Chi Minh city 721400, Vietnam

Ngoc A.T. Pham, Faculty of Biology and Biotechnology, University of Science, 227 Nguyen Van Cu, District 5, Ho Chi Minh City 749000, Vietnam

Vietnam National University, Linh Trung, Thu Duc, Ho Chi Minh city 721400, Vietnam

Quoc T. Tran, Faculty of Biology and Biotechnology, University of Science, 227 Nguyen Van Cu, District 5, Ho Chi Minh City 749000, Vietnam

Vietnam National University, Linh Trung, Thu Duc, Ho Chi Minh city 721400, Vietnam

Huy D. Nguyen, Vietnam National University, Linh Trung, Thu Duc, Ho Chi Minh city 721400, Vietnam

Central Laboratory of Analysis, University of Science, 227 Nguyen Van Cu, District 5, Ho Chi Minh City 749000, Vietnam

Duc H. Nguyen, Faculty of Biology and Biotechnology, University of Science, 227 Nguyen Van Cu, District 5, Ho Chi Minh City 749000, Vietnam

Vietnam National University, Linh Trung, Thu Duc, Ho Chi Minh city 721400, Vietnam

Linh T. Tran, Faculty of Biology and Biotechnology, University of Science, 227 Nguyen Van Cu, District 5, Ho Chi Minh City 749000, Vietnam

Vietnam National University, Linh Trung, Thu Duc, Ho Chi Minh city 721400, Vietnam

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Published

2023-07-31

How to Cite

Nguyen, T. T., Pham, N. A., Tran, Q. T., Nguyen, H. D., Nguyen, D. H., Pham, K. H., … Tran, L. T. (2023). Antioxidant Activities of Enriched Polysaccharide Fractions from Mycelia of Amauroderma subresinosum: http://www.doi.org/10.26538/tjnpr/v7i7.24. Tropical Journal of Natural Product Research (TJNPR), 7(7), 3445–3451. Retrieved from https://tjnpr.org/index.php/home/article/view/2245

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