Antioxidant, Antiglycation, Inhibition of Digestive Enzymes and Enhanced Glucose Uptake Activities of Opuntia ficus indica L, In Vitro and In Vivo http://www.doi.org/10.26538/tjnpr/v7i7.13
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Abstract
Opuntia ficus indica L (Cactaceae), is commonly employed in Morocco as an alternative medicinal approach for managing diabetes mellitus (DM). The objective of the study is to explore and examine novel mechanism underlying the antidiabetic activity of Opuntia ficus indica seed oil (OFIO), which has shown promising potential in managing diabetes, but the exact mechanisms responsible for its antidiabetic effects remain unclear. Therefore, the current study explores the antihyperglycemic effectof OFIO, the in vitro antioxidant properties, and the antiglycation activity of OFIOof hemoglobin (Hb) model. The total phenolic levels were determined using Folin– Ciocalteu colorimetric approach and total flavonoid levels were determined using colorimetric assay with aluminum chloride. The effect of antihyperglycemia was evaluated using the inhibitory effect of pancreatic α-amylase activity both in vitro and in vivo using Alloxan induced-diabetic rats and the glucose uptake in an isolated rat hemidiaphragm in vitro model. The antioxidant
activity was studied using the DPPH Radical scavenging activity test. In addition, the antiglycation activity of OFIO was also tested in-vitro using hemoglobin model. The outcomes derived from this research have demonstrated that OFIO exhibited potent α-amylase inhibitory activity in vitro (p < 0.01; IC50 values of 4.24 ±0.18 mg/ml, and 4.05± 0.08 mg/ml respectively) and in vivo using
Alloxan induced-diabetic rats. In contrast, OFIO has no effect on glucose uptake by the rat diaphragm muscle as compared to negative control. In addition, OFIO showed a significant antioxidant and hemoglobin antiglycation activities (p<0.001) with an IC50 value of 2.05± 0.06 mg/ml, and 0.485± 0.017 mg/ml, respectively.The findings of this study suggest that is OFIO has promising antidiabetic activity.
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