Alkaloids from Marrubium vulgare L.: Antioxidant and Anti-Inflammatory Activities as a Function of Extraction Methods

http://www.doi.org/10.26538/tjnpr/v7i7.120

Authors

  • Taha Ouriagli Human Pathology, Biomedicine and Environment Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohamed Ben Abdallah University, Fez, Morocco
  • Abdellatif Amnay Human Pathology, Biomedicine and Environment Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohamed Ben Abdallah University, Fez, Morocco
  • Sidi M. Raoui Laboratory of Functional Ecology and Engineering Environment, Faculty of Sciences and Technologies, Sidi Mohamed Ben Abdellah University, Fez, Morocco
  • Faouzi Errachidi Human Pathology, Biomedicine and Environment Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohamed Ben Abdallah University, Fez, Morocco
  • Fouad O. Chahdi Laboratory of Applied Organic Chemistry, Faculty of Sciences and Technologies, Sidi Mohammed Ben Abdallah University, Fez, Morocco
  • Rachida Chabir Human Pathology, Biomedicine and Environment Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohamed Ben Abdallah University, Fez, Morocco

Keywords:

anti-inflammatory activity, antioxidant activity, column chromatography, alkaloids, extraction methods, Marrubium vulgare L., medicinal plants

Abstract

Marrubium vulgare L, commonly known as white horehound, is a medicinal plant widely recognized for its therapeutic properties. It has been extensively studied for its bioactive compounds, including alkaloids, which possess significant antioxidant and anti-inflammatory activities. This study investigates the impact of four extraction methods (soxhlet, refluxing, maceration A, and maceration B) on the antioxidant and anti-inflammatory activities of alkaloids from Marrubium vulgare L. The qualitative analysis reveals Emetine and Cephaeline as the predominant alkaloid compounds in the plant leaf extracts. Among the methods, soxhlet and
maceration B demonstrate the highest extraction yields (9.81% and 9.57% respectively). Notably, maceration B exhibits exceptional antioxidant activity as ascorbic acid equivalent per gram of dry matter (2370.53 AAE/1gDM), while soxhlet exhibits remarkable anti-inflammatory activity (78.51% inhibition at 500 mg/kg, 180 minutes post-formol injection). These findings underscore the importance of maceration B and soxhlet methods for enhancing alkaloid extraction yield and bioactivity. 

Author Biographies

Taha Ouriagli, Human Pathology, Biomedicine and Environment Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohamed Ben Abdallah University, Fez, Morocco

Laboratory of Functional Ecology and Engineering Environment, Faculty of Sciences and Technologies, Sidi Mohamed Ben Abdellah University, Fez, Morocco

Abdellatif Amnay, Human Pathology, Biomedicine and Environment Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohamed Ben Abdallah University, Fez, Morocco

Laboratory of Functional Ecology and Engineering Environment, Faculty of Sciences and Technologies, Sidi Mohamed Ben Abdellah University, Fez, Morocco

Sidi M. Raoui, Laboratory of Functional Ecology and Engineering Environment, Faculty of Sciences and Technologies, Sidi Mohamed Ben Abdellah University, Fez, Morocco

Higher Institute of Nursing professions and Health Techniques of Fez-Meknes, Morocco

Faouzi Errachidi, Human Pathology, Biomedicine and Environment Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohamed Ben Abdallah University, Fez, Morocco

Laboratory of Functional Ecology and Engineering Environment, Faculty of Sciences and Technologies, Sidi Mohamed Ben Abdellah University, Fez, Morocco

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Published

2023-07-31

How to Cite

Ouriagli, T., Amnay, A., Raoui, S. M., Errachidi, F., Chahdi, F. O., & Chabir, R. (2023). Alkaloids from Marrubium vulgare L.: Antioxidant and Anti-Inflammatory Activities as a Function of Extraction Methods: http://www.doi.org/10.26538/tjnpr/v7i7.120. Tropical Journal of Natural Product Research (TJNPR), 7(7), 3411–3420. Retrieved from https://tjnpr.org/index.php/home/article/view/2234