Exploring Natural Essence: Aromatic Compounds and Antioxidant Potency in Concretes from Nong Khuang Forest Plants, Nasinuan Subdistrict, Maha Sarakham Province, Thailand



  • Nutkamon Tantaisong Natural Antioxidant Innovation Research Unit, Department of Biotechnology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand
  • Rungnapa Khrueawan Natural Antioxidant Innovation Research Unit, Department of Biotechnology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand
  • Chadaporn Senakun Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham 44150, Thailand
  • Luchai Butkhup Natural Antioxidant Innovation Research Unit, Department of Biotechnology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand
  • Issaraporn Somboonwatthanakul Natural Antioxidant Innovation Research Unit, Department of Biotechnology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand


medicinal plants, ABTS, Hydroxyl radical assay, DPPH, essential oil


The Nasinuan Community Forest is publicly accessible and conserved to ensure sustainable utilization, guided by the royal initiative spearheaded by Her Royal Highness Princess Maha Chakri Sirindhorn. Previous studies have highlighted the remarkable presence of microbial and plant biodiversity in this forest, suggesting that the contained plant species may possess distinctive attributes. This study investigated the volatile organic compositions (VOCs) and antioxidant activity of concretes derived from plants within this forest.
Nineteen varieties were extracted using hexane. Headspace technique of gas chromatographymass spectrometry identified various antioxidants and compounds, including 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6 in Ellipanthus tomentosus subsp. tomentosus and acetic acid in several species. Octodrine, a weight loss dietary supplement, was detected in Acacia auriculiformis and Dipterocarpus obtusifolius. Promising antioxidant capacity was observed in Pentacme siamensis, Xylia xylocarpa var. kerrii, Breynia glauca, and Peltophorum dasyrachis var. dasyrhachis, displaying moderate inhibition activity by ºOH (hydroxyl radical scavenging)
assay. Furthermore, Shorea obtusa and Dipterocarpus obtusifolius exhibited high inhibition activity of over 80% by DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. B. glauca and Amorphophallus brevispathus exhibited the highest inhibitory activity levels >85% by ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay. Notably, Naringi crenulata measured the highest activity levels by the ferric-reducing antioxidant power (FRAP) assay. This study presents the first documentation of the antioxidant abilities of concretes derived from the bark of Sindora siamensis var. maritima and Shorea obtusa. However, further research and development are required to explore the pharmaceutical and cosmetic applications of these potent plant-derived concretes with high antioxidant capacities.  


Egharevba, E, Chukwuemeke-Nwani, P, Eboh, U, Okoye, E, Bolanle, IO, Oseghale, IO, Imieje,VO, Erharuyi,O, Falodun, A. Evaluation of the antioxidant and hypoglycaemic

potentials of the leaf extracts of Stachytarphyta jamaicensis (Verbenaceae). Trop J Nat Prod Res. 2019; 3(5):170-174.

Essien EE, Thomas PS, Oriakhi K, Choudhary MI. Characterization and antioxidant activity of volatile constituents from different parts of Aframomum danielli

(Hook) K. Schum. Medicines. 2017; 4(2):29.

Foss K, Przybyłowicz KE, Sawicki T. Antioxidant activity and profile of phenolic compounds in selected herbal plants. Plant Foods Hum Nutr. 2022; 77(3):383-389.

Gülcin I. Antioxidant activity of food constituents: an overview. Arch Toxicol. 2012; 86: 345-391.

Sadgrove NJ, Padilla-González GF, Phumthum M. Fundamental chemistry of essential oils and volatile organic compounds, methods of analysis and authentication. Plants.

; 11(6):789.

Mousavi SA, Nateghi L, Javanmard DM, Ramezan Y, PiraviVanak Z, Paidari S, Mohammadi NA. Effects of incorporation of Chavir ultrasound and maceration extracts

on the antioxidant activity and oxidative stability of ordinary virgin olive oil: identification of volatile organic compounds. J Food Meas Charact. 2022; 16(5): 4236-4250.

Piesik D, Aksoy J, Łyczko J, Bocianowski J, Buszewski B, Piesik M, Mayhew CA. Relationships between volatile organic compounds released by wheat plants following

artificial stress and their potential influence on natural pest management. Appl Sci. 2022; 12(15): 7762.

Sadgrove NJ, Padilla-González GF, Phumthum M. Fundamental chemistry of essential oils and volatile organic compounds, methods of analysis and authentication. Plants.

; 11(6):789.

Luang-In V, Yotchaisarn M, Saengha W, Udomwong P, Deeseenthum, S, Maneewan K. Isolation and identification of amylase-producing bacteria from soil in Nasinuan

Community Forest, Maha Sarakham, Thailand. Biomed Pharmacol J. 2019; 12(3): 1061-1068.

Luang-In V, Yotchaisarn M, Saengha W, Udomwong P, Deeseenthum S, Maneewan K. Protease-producing bacteria from soil in Nasinuan Community Forest, Mahasarakham

province, Thailand. Biomed Pharmacol J. 2019; 12(2): 587- 595.

Luang-In V, Saengha W, Deeseenthum S, Maneewan K, Udomwong P. Identification of soil bacteria isolated from Nasinuan Community Forest with potential application in

agriculture. J Sustain Sci Manag. 2021; 16:153-165.

Ubeda C, Callejón RM, Hidalgo C, Torija MJ, Mas A, Troncoso AM, Morales ML. Determination of major volatile compounds during the production of fruit vinegars by static

headspace gas chromatography mass spectrometry method. Food Res Int. 2011; 44(1): 259-268.

de Sousa Galvão M, Narain N, dos Santos MDSP, Nunes ML. Volatile compounds and descriptive odor attributes in umbu (Spondias tuberosa) fruits during maturation. Food

Res Int. 2011; 44(7):1919-1926.

The National Institute of Standards and Technology. NIST Chemistry WebBook, SRD 69. [Online]. 2023 [cited 2023 March 10]. Available from:


Halliwell B, Gutteridge JM, Aruoma OI. The deoxyribose method: a simple “test-tube” assay for determination of rate constants for reactions of hydroxyl radicals. Anal

Biochem.1987; 165(1):215-219.

Waranusantigul P, Thophon S, Phaenark C, Kangwanrangsan N, Hanphakphoom S. Antioxidant study of crude extract from Chromolaena odorata (L) King&

Robinson. SDU Res J. 2016; 9(2):31-58.

Saengha W, Karirat T, Buranrat B, Matra K, Deeseenthum S, Katisart T, Luang-In V. Cold plasma treatment on mustard green seeds and its effect on growth, isothiocyanates,

antioxidant activity and anticancer activity of microgreens. Int J Agric Biol. 2021; 25: 667-676.

Sridhar K, Charles AL. In vitro antioxidant activity of Kyoho grape extracts in DPPH and ABTS assays: Estimation methods for EC50 using advanced statistical programs. Food

Chem. 2019; 275:41-49.

Kadchumsang S, Sirisa-Ard P, Sookkhee S, Chansakaow S. Antibacterial and antioxidant activities of Lanna medicinal plants used in Mahoog formula. Int J Pharm Pharm Sci. 2015;

(9): 366-370.

Phromnoi K, Sinchaiyakij P, Khanaree C, Nuntaboon P, Chanwikrai Y, Chaiwangsri T, Suttajit M. Antiinflammatory and antioxidant activities of medicinal plants

used by traditional healers for antiulcer treatment. Sci Pharm. 2019; 87(3):22.

Suksungworn R, Choowongkomon K, Duangsrisai S. In vitro anti-oxidant, anti-microbial and anti-HIV-1 reverse transcriptase activities and isolation of Bergenin from Shorea

obtusa Wall. ex Blume. Agric Nat Resour. 2021; 55(3):395- 404.

Saadullah M, Asif M, Arif S, Kanwal B. A comprehensive review on traditional uses, chemical constituents, and diverse pharmacological importance of the genus Breynia. Rec Nat

Prod. 2022; 16(6): 538-549.

Tokaew W, Sukpuangkaew N, Promprasit P. Plant diversity and utilization in Kok Si Community Forest, Muang district, Maha Sarakham province. J Sci Tech RMU. 2021; 4(1):1-16.

Sarada K, Margret RJ, Mohan VR. Hepatoprotechtive and antioxidant activity of ethanol extracts of Naringi crenulata (Roxb) Nicolson against CCl4 induced hepatotoxicity in rats.

Int J Pharm Sci Res. 2012; 3(3):874-880.

Lopez-Bucio J, Nieto-Jacobo, MF, Ramırez-Rodrıguez V, Herrera-Estrella L. Organic acid metabolism in plants: from adaptive physiology to transgenic varieties for cultivation in

extreme soils. Plant Sci. 2000; 160(1):1-13.

Kasahara H. Current aspects of auxin biosynthesis in plants. Biosci Biotechnol Biochem. 2016; 80(1):34-42.

Chen D, Shao M, Sun S, Liu T, Zhang H, Qin N, Zeng R, Song, Y. Enhancement of jasmonate-mediated antiherbivore defense responses in tomato by acetic acid, a potent inducer

for plant protection. Front Plant Sci. 2019; 10(764):1-9.

Catalani V, Prilutskaya M, Al-Imam A, Marrinan S, Elgharably Y, Zloh M, Martinotti G, Chilcott R, Corazza O. Octodrine: New questions and challenges in sport

supplements. Brain Sci. 2018; 8(2):34.

Parveen M, Azaz S, Ali A, Basudan OA, Pereira Silva PS, Vivas CY. A novel 6-hydroxy-8,11,11-trimethylbicyclo[7.2.0]undec-4-ene-4- carboxylic acid: a potent

antioxidant agent from Iphiona scabra, J Asian Nat Prod Res. 2014; 16(4):406-411.

Yu X, Zhao M, Liu F, Zeng S. Identification of 2,3-dihydro- 3,5-dihydroxy-6-methyl-4H-pyran-4-one as a strong antioxidant in glucose–histidine Maillard reaction products.

Food Res Int. 2013; 51(1): 397–403.

Mijin DZ, Misic-Vukovic MM, Prtrovic SD. Alkylation of N-substituted 2-phenylacetamides. J Serb Chem Soc. 2004; 69(10):711-736.

de Roos J, De Vuyst L. Acetic acid bacteria in fermented foods and beverages, Curr Opin Biotechnol. 2018; 49:115- 119.

Kolar P, Kastner JR. Low-temperature catalytic oxidation of aldehyde mixtures using wood fly ash: kinetics, mechanism, and effect of ozone. Chemosphere. 2010; 78: 1110-1115.

Ardizzoni A, Hansen H, Dombernowsky P. Topotecan: a new active drug in the second-line treatment of small cell lung cancer. A phase II study in patients with refractory and

sensitive disease. J Clin Oncol. 1997; 15:2090-2096.

Rashid TS. Bioactive metabolites from tomato endophytic fungi with antibacterial activity against tomato bacterial spot disease. Rhizosphere. 2021; 17: 100292.

Afzal MI, Ariceaga CCG, Boulahya KA, Jacquot M, Delaunay S, Cailliez-Grimal C. Biosynthesis and role of 3- methylbutanal in cheese by lactic acid bacteria: major

metabolic pathways, enzymes involved, and strategies for control. Crit Rev Food Sci Nutr. 2017; 57(2):399-406.

Ekade PP, Manik SR. Determination of phytoconstituents of Dolichandrone falcata Seem. Flower through GC-MS. Int j pharm res scholars. 2014; 3(1):573-580.

Le Berre E, Atanasova B, Langlois D, Etievant P, ThomasDanguin T. Impact of ethanol on the perception of wine odorant mixtures. Food Qual Prefer. 2007; 18(6): 901-908

Church JM, Joshi HK. Acetaldehyde by dehydrogenation of ethyl alcohol. Ind Eng Chem. 1951; 43(8): 1804-1811.

Cofer TM, Seidl-Adams I, Tumlinson JH. From acetoin to (Z)-3-hexen-1-ol: the diversity of volatile organic compounds that induce plant responses. J Agric Food Chem.

; 66(43): 11197-11208.

Nie CN, Gao Y, Du X, Bian JL, Li H, Zhang X, W, CM, Li, SY. Characterization of the effect of cis-3-hexen-1-ol on green tea aroma. Sci Rep. 2020; 10(1): 15506.

Bharath B, Perinbam K, Devanesan S, AlSalhi MS, Saravanan M. Evaluation of the anticancer potential of Hexadecanoic acid from brown algae Turbinaria ornata on

HT–29 colon cancer cells. J Mol Struct. 2021; 1235: 130229.

Krishnaveni M, Dhanalakshmi R, Nandhini N. GC-MS analysis of phytochemicals, fatty acid profile, antimicrobial activity of Gossypium seeds. Int J Pharm Sci Rev Res. 2014;

(1): 273-276.

Ameen MH, Hadi NA, Sadoon BZ. Production of acetone. [online] 2016. [Cited 2023 May 5]. Available from Chromeextension://efaidnbmnnnibpcajpcglclefindmkaj/https://www


Allen JG, Flanigan SS, LeBlanc M, Vallarino J,MacNaughton P, Stewart JH, Christiani DC. Flavoring chemicals in E-cigarettes: Diacetyl, 2,3-pentanedione, and

acetoin in a sample of 51 products, including fruit-, candy-, and cocktail-flavored E-cigarettes. Environ Health Perspect.2016; 124(6): 733-739.

Hallagan JB. The use of diacetyl (2,3-butanedione) and related flavoring substances as flavorings added to foods—Workplace safety issues. Toxicol. 2017; 388: 1-6.

Rychlik M, Schieberle P, Grosch W. Compilation of odor thresholds, odor qualities and retention indices of key food odorants. Germany: Deutsche Forschungsanstalt fu¨r

Lebensmittelchemie, Garching; 1998. 63 p.

Garros SB, Razafindramboa N, Pavia AA. Synthesis of (Z)- 3-hexen-1-yl butyrate by esterification in hexane and in solvent free medium using lipases from Mucor miehei and

Candida antarctica lipase. J Am Oil Chem Soc. 1997; 74:1471-1475.

Ahmed A, Ayoub K, Chaima AJ, Hanaa L, Abdelaziz C. Effect of drying methods on yield, chemical composition and bioactivities of essential oil obtained from Moroccan Mentha

pulegium L. Biocatal Agric Biotechnol 2018; 16:638-643.

Shahar Yar M, Ahmad Siddiqui A, Ashraf Ali M. Synthesis and antimycobacterial activity of novel heterocycles. J Serb Chem Soc. 2007; 72(1):5-11.

Bellina F, Rossi R. Synthesis and biological activity of pyrrole, pyrroline and pyrrolidine derivatives with two aryl groups on adjacent positions. Tetrahedron, 2006; 62:7213-56.

Kula J, Sadowska H. Unsaturated aliphatic C9-aldehyde as natural flavorants. Perfum flavor. 1993; 18:23-25.

Gertsch J, Leonti M, Raduner S, Racz I, Chen JZ, Xie XQ, Altmann KH, Karsak M, Zimmer A. Beta-caryophyllene is a dietary cannabinoid. Proc Natl Acad Sci USA. 2008;

(26): 9099-9104.

National Library of Medicine. Pubchem. [Online]. 2023[cited 2023 March 10]. Available from: https://pubchem.ncbi.nlm.nih.gov/



How to Cite

Tantaisong, N., Khrueawan, R., Senakun, C., Butkhup, L., & Somboonwatthanakul, I. (2023). Exploring Natural Essence: Aromatic Compounds and Antioxidant Potency in Concretes from Nong Khuang Forest Plants, Nasinuan Subdistrict, Maha Sarakham Province, Thailand: http://www.doi.org/10.26538/tjnpr/v7i7.19. Tropical Journal of Natural Product Research (TJNPR), 7(7), 3402–3410. Retrieved from https://tjnpr.org/index.php/home/article/view/2231