Evaluation of Cytotoxicity and Antimicrobial Activity of Flavipin Against Acinetobacter baumannii, Methicillin-Resistant Staphylococcus aureus, and Candida albicans: http://www.doi.org/10.26538/tjnpr/v7i7.2

Mohammed J. Qasim; Azhar A.F. Al-Attraqchi; Gazi M. Aziz

Authors

Mohammed J. Qasim Department of Microbiology, College of Medicine, Al-Nahrain University, Baghdad- Iraq
Azhar A.F. Al-Attraqchi Department of Microbiology, College of Medicine, Al-Nahrain University, Baghdad- Iraq
Gazi M. Aziz Department of Biotechnology, College of Science, Baghdad University, Baghdad-Iraq

Keywords:

Methicillin-resistant Staphylococcus aureus, IC50, Flavipin, Candida albicans, Aspergillus flavus, Antimicrobial activity, Acinetobacter baumannii

Abstract

Flavipin, a metabolite produced by Aspergillus species is a potent antibiotic, antifungal, anticancer, and nematocidal agent. The present study was conducted to investigate the cytotoxicity and antimicrobial activity of flavipin produced by Aspergillus flavus. Eighty isolates of Aspergillus species were screened for the presence of flavipin. Macroscopic, microscopic, and molecular characterizations were performed to identify the Aspergillus species. Flavipin extract was prepared, fractionated, and the concentration was determined in the selected isolates. The antimicrobial activity of flavipin was tested against Candida albicans, Acinetobacter baumannii, and methicillin-resistant Staphylococcus aureus (MRSA). The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC) were determined. A cytotoxicity test of flavipin was conducted on MCF-7 and WRL-68 cell lines. The results of the antibacterial activity of flavipin against MRSA and A. baumannii showed a wide zone of inhibition of 35 and 30 mm, respectively. Flavipin exhibited the highest antifungal activity against C. albicans, with inhibition zones of 44 mm in diameter. The MICs of flavipin for both A. baumannii and MRSA were 31.2 μg/mL, while the MIC of flavipin for C. albicans was 62.5 μg/mL. The MBC values of flavipin for MRSA and A. baumannii were 62.5 and 125 μg/mL, respectively, while the MFC of flavipin against C. albicans was 125 μg/mL. The IC50 of flavipin was 52.34 μg/mL for the cancer cell line MCF-7 and 298.1 μg/mL for the normal cell line WRL-68. The findings of the study revealed that flavipin produced by Aspergillus flavus has antimicrobial and cytotoxic properties.

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