Neuroprotective Activity and Antioxidant Effect of Salacca zalacca Peel Ethanol Extract on High Glucose Induced Zebrafish (Danio rerio) Embryo doi.org/10.26538/tjnpr/v5i12.6
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Abstract
Hyperglycemia in gestational diabetes (GDM) could lead to oxidative stress that result in reduction of superoxide dismutase (SOD) level and sirtuin (SIRT)-1 expression. Reduction of SOD and SIRT-1 inhibit CAMP response element-binding protein (CREB), a regulator of brain derived neurotrophic factor (BDNF) transcription, and reduces BDNF expression. Decreased BDNF expression in newborns could lead to autism spectrum disorder (ASD) and retinopathy of prematurity (ROP) which can interfere with memory and vision function during growth and development. Salacca zalacca (SZ) peel contains antioxidant neuroprotectant substances and this study was aimed to prove the effect of Salacca zalacca peel extract on the development of embryos induced by high glucose. The study used zebrafish embryos induced by 3% of glucose as a model of gestational diabetes. Salacca zalacca peel extract was given at concentrations of 0.1, 0.2, and 0.4 mg/mL. The expression of SIRT-1, BDNF, and SOD of zebrafish embryos was evaluated by reverse transcriptase PCR (RT-PCR). The results showed a significant increase in the expression of SIRT-1, BDNF, and SOD at the concentration of 0.4 mg/mL. It was concluded that Salacca zalacca peel extract has a neuroprotectant and antioxidant effects.
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Egan AM, Vellinga A, Harreiter J, Simmons D, Desoye G, Corcoy R, Adelantado JM, Devlieger R, Van Assche A, Galjaard S, Damm P, Mathiesen ER, Jensen DM, Andersen L, Lapolla A, Dalfrà MG, Bertolotto A, Mantaj U, WenderOzegowska E, Zawiejska A, Hill D, Jelsma JGM, Snoek FJ,
Worda C, Bancher-Todesca D, van Poppel MNM, KautzkyWiller A, Dunne FP; DALI Core Investigator group. Epidemiology of gestational diabetes mellitus according to IADPSG/WHO 2013 criteria among obese pregnant women in Europe. Diabetolog. 2017; 60(10):1913-1921.
Yamamoto JM, Kellett JE, Balsells M, García-Patterson A, Hadar E, Solà I, Gich I, van der Beek EM, CastañedaGutiérrez E, Heinonen S, Hod M, Laitinen K, Olsen SF, Poston L, Rueda R, Rust P, van Lieshout L, Schelkle B, Murphy HR, Corcoy R. Gestational Diabetes Mellitus and
Diet: A Systematic Review and Meta-analysis of Randomized Controlled Trials Examining the Impact of Modified Dietary Interventions on Maternal Glucose Control and Neonatal Birth Weight. Diabet Care. 2018; 41(7):1346-1361.
Turek IA, Wozniak LA, Cypryk K, Wojcik M. Hyperglycaemia-induced oxidative stress in gestational diabetes mellitus (GDM). Diabetol Klin. 2015; 4(5):189-198.
Khotimah H, Wari FE, Noviasari D, Octaviana A, Supriadi RF, Norisa N, Permata TR, Diestika Y, Risnawati, Ali M.M., Nurdiana, Kalsum U, Widodo AM. Centella asiatica alleviates neurotoxicity and development of leadexposed zebrafish larvae. AACL Bioflux 2020; 13(4):1886-
Kitada M and Koya D. SIRT1 in type 2 diabetes: Mechanisms and therapeutic potential. Diabetes Metab J. 2013;37(5):315-325.
Ng F, Wijaya L, Tang BL. SIRT1 in the brain—connections with aging-associated disorders and lifespan. Front Cell Neurosci. 2015; 9:1-13.
Skogstrand K, Hagen CM, Borbye-Lorenzen N, Christiansen M, Bybjerg-Grauholm J, Bækvad-Hansen M, Werge T, Børglum A, Mors O, Nordentoft M, Mortensen PB, Hougaard DM. Reduced neonatal brain-derived neurotrophic factor is associated with autism spectrum
disorders. Transl Psych. 2019; 9(1):252.
Chen SD, Wu CL, Hwang WC, Yang DI. More Insight into BDNF against Neurodegeneration: Anti-Apoptosis, AntiOxidation, and Suppression of Autophagy. Int J Mol Sci. 2017;18(3):545.
Lu B, Nagappan G, Lu Y. BDNF and synaptic plasticity, cognitive function, and dysfunction. Handb Exp Pharmacol. 2014; 220:223-250.
Ighodaro OM and Akinloye OA. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria J Med [Internet]. 2018; 54(4):287-293.
Kantae V, Krekels EH, Ordas A, González O, van Wijk RC, Harms AC, Racz PI, van der Graaf PH, Spaink HP, Hankemeier T. Pharmacokinetic Modeling of Paracetamol Uptake and Clearance in Zebrafish Larvae: Expanding the Allometric Scale in Vertebrates with Five Orders of
Magnitude. Zebrafish. 2016; 13(6):504-510.
Seth A, Stemple DL, Barroso I. The emerging use of zebrafish to model metabolic disease. DMM Dis Model Mech. 2013; 6(5):1080-1088.
Lantz-McPeak S, Guo X, Cuevas E, Dumas M, Newport GD, Ali SF, Paule MG, Kanungo J. Developmental toxicity assay using high content screening of zebrafish embryos. J Appl Toxicol. 2015; 35(3):261-272.
Rohaeti, Eti & Fauzi, M & Batubara, Irmanida. Inhibition of α-Glucosidase, Total Phenolic Content and Flavonoid Content on Skin Fruit and Flesh Extracts of Some Varieties of Snake Fruits. IOP Conference Series: Earth and Environmental Science. 2017.
Qi FY, Yang L, Tian Z, Zhao MG, Liu SB, An JZ. Neuroprotective effects of Asiaticoside. Neural Regen Res. 2014;9(13):1275-1282.
Zhong Z, Han J, Zhang J, Xiao Q, Hu J, Chen L. Pharmacological activities, mechanisms of action, and safety of salidroside in the central nervous system. Drug Des Dev Ther. 2018; 12:1479-1489.
Delcourt J, Ovidio M, Denoël M, Muller M, Pendeville H, Deneubourg J-L, Poncin P. Individual identification and marking techniques for zebrafish. Rev Fish Biol Fish. 2018.28:839–864.
Khotimah H, Putri SNA, Aninditha D, Weningtyas A, Prima W, Kalsum U, Masruroh R, Handayani D, Nandar S. Ethanol extract of Salacca zalacca peel reduce IL-1β and apoptosis in high glucose induced zebrafish embryo. GSC Biol Pharm Sci. 2021; 16(3):024-33.
Craig TA, Zhang Y, McNulty MS, Middha S, Ketha H, Singh RJ, Magis AT, Funk C, Price ND, Ekker SC, Kumar R. Research resource: whole transcriptome RNA sequencing detects multiple 1α,25-dihydroxyvitamin D(3)-sensitive metabolic pathways in developing zebrafish. Mol
Endocrinol. 2012; 26(9):1630-1642.
Abidin IZZ, Fazry S, Jamar NH, Dyari HRE, Ariffin ZZ, Johari AN, Ashaari NS, Johari NA, Abdul Wahab RM, Ariffin SHZ. The effects of Piper sarmentosum aqueous extracts on zebrafish (Danio rerio) embryos and caudal fintissue regeneration. Sci Rep. 2020; 10(1):14165.
Aleström P, D'Angelo L, Midtlyng PJ, Schorderet DF, Schulte-Merker S, Sohm F, Warner S. Zebrafish: Housing and husbandry recommendations. Lab Anim. 2020; 54(3):213-224.
De Felice E, Porreca I, Alleva E, De Girolamo P, Ambrosino C, Ciriaco E, Germanà A, Sordino P. Localization of BDNF expression in the developing brain of zebrafish. J Anat. 2014; 224(5):564-574.
Zakhary SM, Ayubcha D, Ansari F, Kamran K, Karim M, Leheste JR, Horowitz JM, Torres G. A behavioral and molecular analysis of ketamine in zebrafish. Synapse. 2011; 65(2):160-167.
Du J, Cai J, Wang S, You H. Oxidative stress and apotosis to Zebrafish (Danio rerio) embryos exposed to perfluorooctane sulfonate (PFOS) and ZnO nanoparticles. Int J Occup Med Environ Health. 2017; 30(2):213-229.
Kang GG, Francis N, Hill R, Waters D, Blanchard C, Santhakumar AB. Dietary Polyphenols and Gene Expression in Molecular Pathways Associated with Type 2 Diabetes Mellitus: A Review. Int J Mol Sci. 2019; 21(1):140.
Runtuwene J, Cheng KC, Asakawa A, Amitani H, Amitani M, Morinaga A, Takimoto Y, Kairupan BH, Inui A. Rosmarinic acid ameliorates hyperglycemia and insulin sensitivity in diabetic rats, potentially by modulating the expression of PEPCK and GLUT4. Drug Des Devel Ther.
; 10:2193-2202.
Ola MS, Nawaz MI, El-Asrar AA, Abouammoh M, Alhomida AS. Reduced levels of brain derived neurotrophic factor (BDNF) in the serum of diabetic retinopathy patients and in the retina of diabetic rats. Cell Mol Neurobiol. 2013; 33(3):359-367.
Kitada M, Ogura Y, Monno I, Koya D. Sirtuins and type 2 diabetes: Role in inflammation, oxidative stress, and mitochondrial function. Front Endocrinol (Lausanne). 2019; 10:1-12.
Gui J, Potthast A, Rohrbach A, Borns K, Das AM, Von Versen-Hoynck F. Gestational diabetes induces alterations of sirtuins in fetal endothelial cells. Pediatr Res. 2016; 79(5):788-798.
Salminen A, Kaarniranta K, Kauppinen A. Crosstalk between oxidative stress and SIRT1: Impact on the aging process. Int J Mol Sci. 2013; 14(2):3834-3859.
Ng ZX, See AN. Effect of in vitro digestion on the total polyphenol and flavonoid, antioxidant activity and carbohydrate hydrolyzing enzymes inhibitory potential of selected functional plant-based foods. J Food Proc Preserv. 2019; 43(4):1-13.
Andrade JM and Fasolo D. Polyphenol Antioxidants from Natural Sources and Contribution to Health Promotion. Polyphenols Hum Health Dis. 2014; 1:253-265.
Nimmagadda VK, Bever CT, Vattikunta NR, Talat S, Ahmad V, Nagalla NK, et al. Overexpression of SIRT1 Protein in Neurons Protects against Experimental Autoimmune Encephalomyelitis through Activation of Multiple SIRT1 Targets. J Immunol. 2013; 190(9):4595-4607.
Xu J, Jackson CW, Khoury N, Escobar I, Perez-Pinzon MA. Brain SIRT1 mediates metabolic homeostasis and neuroprotection. Front Endocrinol (Lausanne). 2018; 9:1-16.
Dudylina AL, Ivanova MV, Shumaev KB, Ruuge EK. Superoxide Formation in Cardiac Mitochondria and Effect of Phenolic Antioxidants. Cell Biochem Biophys [Internet]. 2019; 77(1):99-107.
Scheper V, Schwieger J, Hamm A, Lenarz T, Hoffmann A. BDNF-overexpressing human mesenchymal stem cells mediate increased neuronal protection in vitro. J Neurosci Res. 2019; 97(11):1414-1429.
Wang D, Li H, Du X, Zhou J, Yuan L, Ren H, Yang X, Zhang G, Chen X. Circulating Brain-Derived Neurotrophic Factor, Antioxidant Enzymes Activities, and Mitochondrial DNA in Bipolar Disorder: An Exploratory Report. Front Psych. 2020; 11:514658.
Khalin I, Alyautdin R, Kocherga G, Bakar MA. Targeted delivery of brain-derived neurotrophic factor for the treatment of blindness and deafness. Int J Nanomed. 2015; 10:3245-3267.
Elbatreek MH, Pachado MP, Cuadrado A, Jandeleit-Dahm K, Schmidt HHHW. Reactive Oxygen Comes of Age: Mechanism-Based Therapy of Diabetic End-Organ Damage. Trends Endocrinol Metab [Internet]. 2019; 30(5):312-327.