Acute Toxicity Studies of a Combination of Ethanol Extracts of Andrographis paniculata, Centella asiatica, and Curcuma heyneana in Rats
doi.org/10.26538/tjnpr/v5i12.5
Keywords:
Acute toxicity, Andrographis paniculata, Centella asiatica, Curcuma heyneanaAbstract
Andrographis paniculata, Centella asiatica, and Curcuma heyneana are beneficial in the treatment of various diseases. A combination of extracts of several medicinal plants increases the potential of the expected effect. The present study was aimed at determining the acute toxicity of a combination of ethanol extracts of A. paniculata, C. asiatica, and C. heyneana in rats. A combination of ethanol extracts of the three plants was prepared. Twenty female rats were divided into 4 groups: the control group, and treatment groups of a combination of extracts at doses of 500, 2,000, and 5,000 mg/kg. The acute toxicity study was carried out on the experimental rats using the Organization for Economic Co-operation and Development (OECD) Guidelines for Testing of Chemicals number 423. The animals were sacrificed on the 15th day, and biochemical analyses were performed on the collected blood samples. Macroscopic and microscopic examinations of the vital organs were determined. The results revealed that there were no toxic symptoms in the female rats. The combination of extracts does not affect the body weight and the relative organ weight of the rats. Also, the biochemical parameters indicated that alanine transaminase (ALT), aspartate transaminase (AST), and creatinine levels increased at the dose of 5,000 mg/kg BW with no significant difference in AST level. The lethal dose (LD50) of the combination of the extracts is higher than 5,000 mg/kg BW. This study found that combining extracts of A. paniculata, C. asiatica, and C. heyneana is relatively non-toxic, with an LD50 greater than 5,000 mg/kg.
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