Ameliorative Potential of Thymoquinone on Male Reproductive Functions in Lead Acetate-Induced Reproductive Toxicity in Male Wistar Rats http://www.doi.org/10.26538/tjnpr/v7i6.24

Main Article Content

Kabiru I. Adedokun
Olayemi O. Oladokun
Tunde F. Abraham
Opeyemi S. Osuntokun
Tope G. Atere
Sakinat T. Baiyewu

Abstract

Lead reproductive toxicity has been reported in men. Nigella sativa seed and its active component, thymoquinone have been used in traditional pharmacopeia as natural remedy to promote reproductive health. This study investigated the ameliorative potential of Thymoquinone (TQ) on male reproductive functions in lead acetate (PbA)-induced reproductive toxicity in Wistar rats. Thirty male Wistar rats weighing 100–120 g were randomly divided into six groups (n=5); Control (distilled water, 0.2 ml/day), PbA, PbA+5 mg/kg TQ, PbA+3.75 mg/kg TQ, PbA+2.5 mg/kg and 5 mg/kg TQ. TQ was given orally at varying doses daily for 8 weeks while PbA (15 mg/Kg) was administered intraperitoneally once per week for 8 weeks. Hypothalamic-pituitary-testicular hormones, sperm analysis, and histological studies of the testis and epididymis were conducted and data were analyzed. A significant decrease (p˂0.05) in GnRH, FSH, LH, and testosterone in PbA, PbA+3.75 mg/kg TQ, and PbA+2.5 mg/kg TQ when compared with the control group. However, GnRH, FSH, LH, and testosterone significantly increased (p<0.05) in PbA+5 mg/kg TQ and PbA+3.75 mg/kg TQ when compared with PbA treated group. Sperm count, motility, viability, and morphology showed significant decrease in PbA treated group when compared with the control, while these were significantly ameliorated following TQ administration. Cytoarchitectural distortions observed in the testis and epididymis in PbA-treated groups were equally improved with TQ administration. Lead acetate induces reproductive toxicity in male Wistar rats via a reduction in hypothalamic-pituitary-testicular hormones, sperm parameters as well as distortion of both testicular and epididymal cytoarchitectures. However, Thymoquinone ameliorated these anomalies.

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Adedokun, K. I., Oladokun, O. O., Abraham, T. F., Osuntokun, O. S., Atere, T. G., & Baiyewu, S. T. (2023). Ameliorative Potential of Thymoquinone on Male Reproductive Functions in Lead Acetate-Induced Reproductive Toxicity in Male Wistar Rats: http://www.doi.org/10.26538/tjnpr/v7i6.24. Tropical Journal of Natural Product Research (TJNPR), 7(6), 3223-3229. https://tjnpr.org/index.php/home/article/view/2108
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How to Cite

Adedokun, K. I., Oladokun, O. O., Abraham, T. F., Osuntokun, O. S., Atere, T. G., & Baiyewu, S. T. (2023). Ameliorative Potential of Thymoquinone on Male Reproductive Functions in Lead Acetate-Induced Reproductive Toxicity in Male Wistar Rats: http://www.doi.org/10.26538/tjnpr/v7i6.24. Tropical Journal of Natural Product Research (TJNPR), 7(6), 3223-3229. https://tjnpr.org/index.php/home/article/view/2108

References

Flora G, Gupta D, Tiwari A. Toxicity of lead: a review with recent updates. Interdisciplinary toxicology. 2012; 1;5(2):47- 58.

Osmel LL, José MSP. Effects of lead on Reproductive Health. Lead chemistry. 2020; 3-20, London. Intech Open.

Santhosh KR, Asha DS. Lead Toxicity on Male Reproductive System and its Mechanism: A Review. Research J. Pharm. and Tech. 2018; 11(3): 1228-1232.

Mustari A, Nooruzzaman M, Miah M A, Sujan K M, Chowdhury E H. Promoting action of vitamin E and black seed oil on reproductive hormones and organ histoarchitecture of Swiss albino mice. Vet. Med. and Sci, 2022; 8:710–718.

Asiyah HA, Syazana NS, Hashida NH, Durriyyah SHA, Kamaruddin MY. Effects of nicotine and gelam honey on testis parameters and sperm qualities of juvenile rats. Sci Res Essays. 2011; 6(26):5471-5474.

Vijayakumar S, Divya M, Vaseeharan B, Chen J, Biruntha M, Silva LP, Dasgupta N. Biological compound capping of silver nanoparticle with the seed extracts of blackcumin (Nigella sativa): a potential antibacterial, antidiabetic, antiinflammatory, and antioxidant. J. Inorg. & Organo. Poly. & Mat., 2021; 31, 624-635.

Bordoni L, Fedeli D, Nasuti C, Maggi F, Papa F, Wabitsch M. Gabbianelli R. Antioxidant and anti-inflammatory properties of Nigella sativa oil in human preadipocytes. Antioxidants, 2019; 8(2), 51.

Darand M, Mirmiran P, Mokari‐Yamchi A. The effect of Nigella sativa on infertility in men and women: A systematic review. Prog in Nut. 2020; 21:33–41

Haseena S, Manjunath A, Kusal K D, Shaik HS. Effects of Thymoquinone on Testosterone and LH levels in Streptozotocine Induced Diabetic male Albino Rats.J. Pharm. Sci. & Res. 2015; 7(8), 554-556.

Sayeed MSB, Shams T, Hossain SF, Rahman MR, Mostofa AGM, Kadir MF, Mahmood S, Asaduzzaman M Nigella sativa L. seeds modulate mood, anxiety and cognition in healthy adolescent males. J Ethnopharmacol 2014; 152:156- 162.

Al-Zahrani S, Mohany M, Kandeal S, Badr G. Thymoquinone and vitamin E supplementation improve the reproductive characteristics of heat-stressed male mice. J Med Plants Res. 2012; 6(3): 493-499.

Rosli FD. Ameliorative Effects of Thymoquinone on Sperm Parameters and Testosterone Level of Nicotine-Treated Sprague Dawley Rats. Braz. Arch. Bio. & Tech. 2019; ISSN 1678-4324.

Ghosh DE, Firdaus SB, Mitra EL, Chattopadhyay AI, Pattari SK, Jana KU, Bandyopadhyay DE. Ameliorative effect of curry leaf aqueous extract against lead acetate-induced oxidative stress in rat kidneys. Int J Pharm Pharm Sci. 2013;5(4):546-56.

Tekeoglu I, Dogan A, Ediz L, Budancamanak M, Demirel A. Effects of thymoquinone (volatile oil of black cumin) on rheumatoid arthritis in rat models. Phytotherapy Research: An Int. J. Devoted to Pharmacol. & Toxicol Eval. of Nat. Pro. Deriv. 2007 Sep;21(9):895-7.

Prasad R, Brodie G, Vanderwoude C, Hodge S. Potential of the weed Abrusprecatorious Linnaeus (Fabale: Fabaceae) for control of insect pests in the south pacific: A review. Int J. Entomo Res. 2016; 3(3):113- 124

World Health Organization. World Health Organization laboratory manual for the examination and processing of human semen. World Health Organization: Geneva, Swtizerland. 2010;287.

Seed J, Chapin RE, Clegg ED. Methods for assessing sperm motility, morphology and counts in rat, rabbit and dogs: A consensus report. Reprod. Toxicol. 1996; 10237- 244.

Adedokun KI, Akande AT, Osuntokun OS, Oladokun OO, Akintayo CO, Atere TG. Evaluation of the Markers of Reproductive Functions in the Male Wistar Rats Treated with Analytical Grade Glyphosate. Trop. J. Health Sci. 2022 Aug 4;29(2):20-6.

Gandhi J, Hernandez R, Chen A, Smith N, Sheynkin Y, Joshi G, Khan S. Impaired hypothalamic-pituitary-testicular axis activity, spermatogenesis, and sperm function promote infertility in males with lead poisoning. Zygote, 2017; 25(2), 103-110. doi:10.1017/S0967199417000028

Winder C. Reproductive and chromosomal effects of occupational exposure to lead in the male. Reprod. Toxicol. 1989; 3:221-233.

Sokol RZ, Wang S, Wan YJ, Stanczyk FZ, Gentzschein E, Chapin RE. Long-term, low-dose lead exposure alters the gonadotropin-releasing hormone system in the male rat. Environ Health Perspect. 2002; 110:871–874.

Wadi SA, Ahmad G. Effects of lead on the male reproductive system in mice. J Toxicol Environ Health. 1999; 56(7):513- 21.

Raji Y, Akinsomisoye OS, Salman TM. Theantispermatogenic activity of Morinda lucida extracts in male rats. Asian J. androl; 2005; 7(4): 405-410.

Zhou YM, Zheng Q, Shi L, Zhang W, Zhen W, Chen YL. An epididymis-specific secretory protein HongrES1 critically regulates sperm capacitation and male fertility. PloS one. 2008; 3(12)4106: 1-12

Graca A, Ramalho-Santos J, de Lourdes PM. Effect of lead chloride on spermatogenesis and sperm parameters in mice. Asian J Androl; 2004; 6: 237-241.

Kasperczyk S, Birkner E, Kasperczyk A, Zalejska-Fiolka J. The activity of superoxide dismutase and catalase in people protractedly exposed to lead compounds. Ann Agric Environ Med. 2004; 11:291–296

Benoff S, Centola GM, Millan C, Napolitano B, Marmar JL, Hurley IR. Increased seminal plasma lead levels adversely affect the fertility potential of sperm in IVF. Human Reprod. 2003; 18:374-383.25.

Yulianto RA, Isnaeni W, Susanti R. Pengaruhpemberian vitamin E terhadap kualitas sperma tikusputih yang dipapar timbal. Unnes J. Life Sci. 2013; 2 2.

Marianto A, Mahatmanti FW. Acta Scientiarum. Bio. Sci 2020; 401.

Kolahdooz M, Nasri S, Modarres SZ, Kianbakht S, Huseini HF. Effects of Nigella sativa L. seed oil on abnormal semen quality in infertile men: a randomized, double-blind, placebo-controlled clinical trial. Phytomedicine. 2014 15;21(6):901-5.

Mahdavi R, Javad H, Nazli N. Effects of black seeds (Nigella sativa) on male infertility. A systematic review. J. Herbal Medicine. 2015; 5(3):133-139

Pandya C, Pillai P, Nampoothiri LP, Bhatt N, Gupta S, Gupta S. Effect of lead and cadmium co-exposure on testicular steroid metabolism and antioxidant system of adult male rats. Andrologia 2012; 44:813–822.

Oyeyemi AW, Anyanwu CP, Akinola AO, Daramola OO, Alli OB, Ehiaghe FA. Clomiphene citrate ameliorated lead acetate-induced reproductive toxicity in male Wistar rats. JBRA Assist Reprod 2019; 23(4):336343

Oyeyemi WA, Daramola OO, Akinola AO, Idris AO, Aikpitanyi I. Hepatic and reproductive toxicity of subchronic exposure to dichlorvos and lead acetate on male Wistar rats. Asian Pacific J Reprod 2020; 9:283–290

Sokol RZ. Hormonal effects of lead acetate in the male rat: Mechanism of action1. Bio. of Reprod. 1987; 37:1135-1138.

Alawa ZM, Al-Omary HL, Jaafar I. Environmental lead exposure and male infertility. IOSR J Dent and Med Sci. 2016; 15:49-54.

Siu ER, Mruk DD, Porto CS, Cheng CY. Cadmium-induced testicular injury. Toxicol Appl Pharmacol. 2009; 2382:240– 249.

Wang X, Wang M, Dong W, Li Y, Zheng X, Piao F, Li S Subchronic exposure to lead acetate inhibits spermatogenesis and down regulates the expression of Ddx3y in testis of mice. Reproductive. Toxicol. 2013; 42:242–250.

Weinbauer GF, Luetjens CM, Simoni M, Nieschlag E. Physiology of Testicular Function. Andrology. Springer, Berlin, Heidelberg. 2010; 11-59.

Uzun FG, Demir FS, Kalender HB, Kalender Y. Protective effect of cat-echin and quercetin on chlorpyrifos-induced lung toxicity in male rats. Food Chem Toxicol 2010; 48:1714–1720.

Daoud S, Tahri A, Rim K, Kamilia K, Tahia B, Zouheir S. Safety assessment of cefuroxime on male reproductive system: Sub-acute toxicity and potential reversibility after a complete cycle of spermatogenesis and epididymal maturation in Wistar rats. Reprod. Toxicol. 2019; 85: 75-82.

Al-Naqeep G, Ismail M, Chan KW. Nigella sativa Thymoquinone rich fraction greatly improves plasma antioxidant capacity and expression of antioxidant genes in hypercholesterolemic rats. Free Radic Biol Med. 2012; 48: 664-72.