Characterization of Chemical Constituents of Adansonia digitata L. using GC-MS and LC-MS/QTOF and their In Vitro Anti-cervical Cancer Effects http://www.doi.org/10.26538/tjnpr/v7i6.19
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Abstract
Plants and their chemical constituents have been utilized in the development of new medicines for diseases of public health importance like cervical cancer.This study was aimed at the characterization of chemical constituents of different parts of Adansonia digitata and their anticervical cancer activity. The chemical constituents of the fruits pulp, leaves, stem bark, and root extracts of the plant were identified using GC-MS and LC-MS/QTOF techniques while the anticervical cancer activity of the extracts was investigated using MTT assay. The GC-MS analysis indicated the presence of 4, 12, 10, and 7 chemical compounds for the fruits pulp, leaves, stem bark, and roots, respectively. While the LC-MS/QTOF analysis revealed the presence of 10, 12, 15, and 17 compounds in the fruits pulp, roots, leaves, and stem-bark, respectively. The major phenolic compounds identified were rutin, quercetin-3-β-d-glucoside, gentisic acid, catechin, and diosmin. The highest amount of phenolic constituent present in the extracts was rutin (0.3192 mg/g of dry plant material) in the leaves. The extracts of A. digitata (50, 100, and 250 µg/mL) have shown a significant cytotoxic effect on the HeLa cell line in a concentration-dependent manner. Interestingly, the leaves at the highest concentration showed an excellent cytotoxic effect with 91.5, 90.8, 85.0, and 82.4% growth inhibition at 12, 24, 36, and 48 hours and IC50 values of 99.17, 104.41, 162.23, and 170.02 µg/mL, respectively. This study indicated the promising potentials of A. digitata leaves as a good source of cytotoxic compounds against cervical cancer for future applications in pharmaceutical industries.
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