Antihyperlipidemic Activity of Purified Polyphenol Extracted from Water Lettuce (Pistia stratiotes) Leaf: An In Vitro Analysis
http://www.doi.org/10.26538/tjnpr/v7i6.17
Keywords:
purification, polyphenol, Pistia stratiotes, AntihyperlipidemicAbstract
Obesity is a metabolic disorder disease and excessive fat accumulation. Synthetic antiobesity (antihyperlipidemic) drugs are considered to cause some side effects. Therefore, the investigation of novel antiobesity agents, with less adverse effects, is a major challenge in the future research, such as from plant extracts. Thus, this research aimed to investigate the antihyperlipidemic of purified polyphenol from water lettuce (Pistia stratiotes) extract. The research was conducted using crude and purified extracts of water lettuce. The purification process was performed by solid-phase extraction by using a HyperSep Retain PEP cartridge. The qualitative analysis of bioactive compounds (flavonoid, alkaloid, terpenoid, tannin) and quantitative analysis of lipase inhibition were conducted in the laboratory. The results showed that flavonoid, alkaloid, and tannin were increased after the purification process, whereas triterpenoid was reduced. The stretching of a polymeric hydroxyl group (O–H) and H-bonded stretching at 3382.36 cm-1 (crude extract) and 3399.25 cm-1 (purified extract) indicate present of phenol in the extracts. The inhibition of lipase enzyme also increased from 37.50% (crude extract) to 72.50% (purified extract). Therefore, purified polyphenol compound from water lettuce (Pistia stratiotes) extract is the potential agent as antihyperlipidemic agent.
References
Zielinska-Blizniewska H, Sitarek P, Merecz-Sadowska A, Malinowska K, Zajdel K, Jablonska M, Sliwinski T, Zajdel R. Plant extracts and reactive oxygen species as two counteracting agents with anti- and pro-obesity properties. Int J. Mol Sci. 2019; 20:4556.
Chait A, den Hartigh LJ. Adipose tissue distribution, inflammation and its metabolic consequences, including diabetes and cardiovascular disease. Front Cardiovas Med. 2020; 7:22.
Laufs U, Parhofer KG, Ginsberg HN, Hegele RA. Clinical review on triglycerides, Eur Heart J. 2020; 41:99-109.
Zhu G, Fang Q, Zhu F, Huang D, Yang C. Structure and function of pancreatic lipase-related protein 2 and its relationship with pathological states. Front Genet. 2021; 12:693538.
Zakaria Z, Othman ZA, Suleiman JB, Jalil NAC, Ghazali WSW, Mohamed M. Protective and therapeutic effects of orlistat on metabolic syndrome and oxidative stress in highfat diet-induced metabolic dysfunction-associated fatty liver disease (MAFLD) in rats: Role on Nrf2 activation. Vet Sci. 2021; 8:274.
Kwon YJ, Kwon GE, Lee HS, Choi MH, Lee JW. The effect of orlistat on sterol metabolism in obese patients. Front Endocrinol. 2022; 13:824269.
Liu TT, Liu XT, Chen QX, Shi Y. Lipase inhibitors for obesity: A review. Biomed Pharmacother. 2020; 128:110314.
Martinez-Gonzalez AI, Alvarez-Parrilla E, Díaz-Sánchez AG, de la Rosa LA, Núñez-Gastélum JA, Vazquez-Flores AA, Gonzalez-Aguilar GA. In vitro inhibition of pancreatic lipase by polyphenols: A kinetic, fluorescence spectroscopy and molecular docking study. Food Tech Biotechnol. 2017; 55:519-530.
Gulua L, Nikolaishvili L, Jgenti M, Turmanidze T, Dzneladze G. Polyphenol content, anti-lipase and antioxidant activity of teas made in Georgia. Ann Agrar Sci. 2018; 16:357-361.
Li S, Pan J, Hu X, Zhang Y, Gong D, Zhang G. Kaempferol inhibits the activity of pancreatic lipase and its synergistic effect with orlistat. J. Funct Foods. 2020; 72:104041.
Hano C, Tungmunnithum D. Plant polyphenols, more than just simple natural antioxidants: oxidative stress, aging and age-related diseases. Medicines. 2020; 7:26.
Generalić Mekinić I, Skroza D, Šimat V, Hamed I, Čagalj M, Popović Perković Z. Phenolic content of brown algae (Pheophyceae) species: Extraction, identification, and quantification. J Biomolecules. 2019; 9:244.
Herpandi, Lestari SD, Bastian, Sudirman S. Antioxidant activity of the fractions from water lettuce (Pistia stratiotes) extract. Food Res. 2021; 5:451-455.
Sudirman S, Herpandi, Safitri E, Apriani EF, Taqwa FH. Total polyphenol and flavonoid contents and antioxidant activities of water lettuce (Pistia stratiotes) leave extracts. Food Res. 2022; 6:205-210.
Sudirman S, Janna M, Herpandi, Widiastuti I. In vitro inhibitory HMG-CoA reductase activity of purified polyphenol compounds from water lettuce (Pistia stratiotes) leaf extract. Trop J. Nat Prod Res. 2022; 6:1131-1134.
Pérez-Magariño S, Ortega-Heras M, Cano-Mozo E. Optimization of a solid-phase extraction method using copolymer sorbents for isolation of phenolic compounds in red wines and quantification by HPLC. J. Agric Food Chem. 2008; 56:11560-11570.
Usman H, Abdulrahman FI, Usman A. Qualitative phytochemical screening and in vitro antimicrobial effects of methanol stem bark extract of Ficus thonningii (Moraceae). Afr J Tradit Complement Altern Med. 2010; 6:289-295.
Laveena KB, Chandra M. Qualitative phytochemical screening of selected medicinal plants of Dakshina Kannada District. Int J. Adv Res. 2020; 8:506-511.
Dilek D, Udoh AU, Ozer TB, Akbulut A, Erkaya IA, Yildiz K, Guler D. Fourier transform infrared (FTIR) spectroscopy for identification of Chlorella vulgaris Beijerinck 1890 and Scenedesmus obliquus (Turpin) Kützing 1833. Afr J Biotechnol. 2012; 11:3817-3824.
Yunarto N, Aini N, Sulistyowati I, Oktoberia IS, Kurniatri AA. Antioxidant activity along with inhibition of HMG CoA reductase and lipase from Anredera cordifolia leaf - Curcuma xanthorrhiza rhizome combination. Pharm J. Indones. 2019; 9:89-96.
Valencia-Avilés E, García-Pérez M, Garnica-Romo M, Figueroa-Cárdenas J, Meléndez-Herrera J, SalgadoGarciglia R, Martínez-Flores H. Antioxidant properties of polyphenolic extracts from Quercus laurina, Quercus crassifolia, and Quercus scytophylla Bark. Antioxidants. 2018; 7:81.
Hu JP, Rena K, Wang XM, Wang XQ, Yang JH. Extraction and purification of total polyphenols from Elaeagnus angustifolia seeds. Food Sci. 2010; 31:123-126.
Nugroho AE, Malik A, Pramono A. Total phenolic and flavonoid contents, and in vitro antihypertension activity of purified extract of Indonesian cashew leaves (Anacardium occidentale L.). Int Food Res J. 2013; 20:299-305.
Lucci P, Saurina J, Núñez O. Trends in LC-MS and LCHRMS analysis and characterization of polyphenols in food. TrAC Trends Anal Chem. 2017; 88:1-24.
Truong DH, Nguyen DH, Ta NTA, Bui AV, Do TH, Nguyen HC. Evaluation of the use of different solvents for phytochemical constituents, antioxidants, and in vitro antiinflammatory activities of Severinia buxifolia. J. Food Qual. 2019; 2019:8178294.
Jiang Z, Kempinski C, Chappell J. Extraction and analysis of terpenes/terpenoids. Curr Protoc Plant Biol. 2016; 1:345- 358.
Wongsa P, Phatikulrungsun P, Prathumthong S. FT-IR characteristics, phenolic profiles and inhibitory potential against digestive enzymes of 25 herbal infusions. Sci Rep. 2022; 12:6631.
Kannan RRR, Arumugam R, Perumal A. Fourier transform infrared spectroscopy analysis of seagrass polyphenols. Curr Bioact Compd. 2011; 7:118-125.
Pereira ES, Vinholes JR, Camargo TM, Raphaelli CO, Ferri NML, Nora L, Vizzotto M. Araçá (Psidium cattleianum Sabine): Bioactive compounds, antioxidant activity and pancreatic lipase inhibition. Ciência Rural. 2021; 51:e20200778.
Angeloni S, Spinozzi E, Maggi F, Sagratini G, Caprioli G, Borsetta G, Ak G, Sinan KI, Zengin G, Arpini S, Mombelli G, Ricciutelli M. Phytochemical profile and biological activities of crude and purified Leonurus cardiaca extracts. Plants. 2021; 10:195.
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