Exploring the Polyphenol Contents and Antioxidant Capacity of the Leaf Extracts of Selected Indonesian Syzygium Species http://www.doi.org/10.26538/tjnpr/v7i6.8
Main Article Content
Abstract
The Myrtaceae family has about 3000 species of fruit-producing trees. The edible fruits of these trees are widely consumed by the Indonesian people. Some of the plants belonging to this family are various guavas from the genus Syzygium. Traditionally, the guava plant is used to treat diarrhoea. It has been shown to possess antidiabetic, antimicrobial, antihypertensive, and antioxidant activities. The present study is aimed at exploring the polyphenolic contents and antioxidant activity of the leaves of three types of guavas; Syzygium aqueum (Burm.f.) Alston, Syzygium malaccense (L.) Merr. and Perry., Syzygium samarangense (Blume) Merr. & L.M.
Perry, and compared to Psidium guajava leaves. The polyphenolic contents (total tannins, total flavonoids, and total phenols) of the four types of guava leaves were determined using standard methods. Qualitative determination of phenolics, flavonoids, and other organic components of these plants were also carried out using thin layer chromatography (TLC). The antioxidant capacity was measured by the phosphomolybdate method using quercetin as the reference standard. The results showed that the highest phenol and tannin content was found in Syzygium aqueum leaves compared to two other types of guavas from the genus Syzygium. The TLC chromatogram showed similarity in the organic components of the three types of guavas from the genus Syzygium. The antioxidant activity was exhibited by Syzygium aqueum leaves could be related to its high phenolic and tannin content.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Gayen P, Ama H, Saifuzzaman M, Faroque A. Anthelmintic activity of ethanolic extract of Syzygium samarangense(Blume) Merril & Perry. Dhaka Univ J Pharm Sci. 2016; 15:109–111.
Kafle A, Mohapatra SS, Reddy I, Chapagain M. A review on medicinal properties of Psidium guajava. J Med Plants Stud. 2018; 6(4):44–47.
Tilaar M, Widjaja B, Ranti A. The Green Science of Jamu. PT Dian Rakyat, editor. Jakarta: PT Dian Rakyat; 2010.
The National Agency of Drug and Food Control (NA-DFC). Statistik Produk Yang Mendapat Persetujuan Izin Edar [Internet]. https://cekbpom.pom.go.id. 2021. Available from: https://cekbpom.pom.go.id//home/produk/p5i4kt4kfpe75r9p
o7ng9gqap7/all/row/10/page/2/order/4/DESC/search/5/psidii
Aung E, Kristanti A, Aminah N, Takaya Y, Ramadhan R. Plant description, phytochemical constituents and bioactivities of Syzygium genus: A review. Open Chem. 2020; 18(1):1256–1281.
Group S working. Syzygium (Myrtaceae): Monographing a taxonomic giant via 22 coordinated regional revisions. PeerJ Prepr [Internet]. 2016;(4:e1930v1). Available from: https://doi.org/10.7287/peerj.preprints.1930v1
Kokilananthan S, Bulugahapitiya VP, Manawadu H,
Gangabadage CS. Comparative Evaluation of Different Extraction Techniques on Phytochemicals and Antioxidant Activity of Psidium Guajava L. Leaves. Trop J Nat Prod Res. 2022; 6(4):552–557.
Itam A, Wati MS, Agustin V, Sabri N, Jumanah RA, Efdi M. Comparative Study of Phytochemical, Antioxidant, and Cytotoxic Activities and Phenolic Content of Syzygium aqueum (Burm. f. Alston f.) Extracts Growing in West Sumatera Indonesia. Hindawi. Sci World J Vol. 2021;1–9.
Arumugam B, Manaharan T, Heng C, Kuppusamy U. Antioxidant and Antiglycemic Potentials of a Standardized Extract of Syzygium malaccense. Food Sci Technol. 2014; 59(2):707–712.
Choironi N and Fareza M. Phytochemical screening and antibacterial activity of ethanolic extract of Syzygium samarangense leaves. J Kartika Kim. 2018; 1(1):1–4.
Majumder R, Nur-E-Hasnat, Ashraf-Uz-Zaman M, Alam M. In vivo evaluation of the pharmacological activities of Syzygium samarangense (Blume) Merr. & L.M. Perry. Adv Biol Res (Rennes). 2014; 8(3):107–115.
Yang J, Paulino R, Janke-Stedronsky S, Abawi F. Freeradical-scavenging Activity and Total Phenols of Noni (Morinda citrifolia L.) Juice and Powder in Processing and Storage. Food Chem. 2007; 102:302–308.
Chang C, Yang M, Wen H, Chern J. Estimation of Total Flavonoid Cintent in Propolis by Two Complementary Colorimetric Methods. J Food Drug Anal. 2002; 10(3):178–182.
Medini F, Fellah H, Ksouri R, Abdelly C. Total phenolic, flavonoid and tannin contents and antioxidant and antimicrobial activities of organic extracts of shoots of the plant Limonium delicatulum. J Taibah Univ Sci. 2014; 8(3):216–224.
Salamah N and Farahana L. Antioxidant activity Assay of Ethanolic Extract of Centella asiatica (L.) Urb Herb using Phosphomolybdate method. Pharmaciana [Internet]. 2014; 4(1):23–30.
Waksmundzka-hajnos M, Sherma J, Kowalska T. Thin Layer Chromatography in Phytochemistry. Cazes J, editor. CRC Press, Taylor & Francis Group; 2008. 888 p.
Rafi M, Heryanto R, Septaningsih DA. “ATLAS” Kromatografi Lapis Tipis Tumbuhan Obat Indonesia (In Bahasa), Thin Layer Chromatography of Indonesian Medicinal Plants. In: Volume 1. Bogor, Indonesia: Pusat Studi Biofarmaka Tropika, LPPM IPB; 2017. 26 p.
Sarker S, Latif Z, Gray A. Natural Products Isolation. Second. New Jersey: Humana Press; 2006.
Hanani E. Pharmacognosy. Jakarta: Penerbit Uhamka Press; 2021. 177–178 p.
Hikmawanti NPE, Fatmawati S, Arifin Z, Vindianita. Pengaruh Variasi Metode Ekstraksi Terhadap Perolehan Senyawa Antioksidan Pada Daun Katuk (Sauropus androgynus (L.) Merr). J Farm Udayana. 2021; 10(1):1.
Hikmawanti NPE, Hanani E, Sapitri Y, Ningrum W. Total phenolic content and antioxidant activity of different extracts of Cordia sebestena L. leaves. Pharmacogn J. 2020; 12(6):1311–1316.
Senet M, Raharja I, Darma I, Prastakarini K, Dewi N, Parwata I. Determination of total flavonoid and total phenol content of kersen root (Mutingia calabura) and the antioxidant activity. J Chem. 2018; 12(1), 13-18.
Achmad S, Hakim E, Makmur L, Syah Y, Juliawaty L, Mujahidin D. Tumbuh-tumbuhan Obat Indonesia Jilid 1. Penerbit ITB, editor. Bandung: Penerbit ITB; 2008.
Hanani E. Analisis Fitokimia (In Bahasa) “Phytochemistry Analysis.” Jakarta: EGC Medical Publisher; 2015; 69(83)110, 148–149, 202, 232–233 p.
Ministry of Health Republic of Indonesia. Farmakope Herbal Indonesia Edisi II. Jakarta: Ministry of Health Republic of Indonesia; 2017. 5–6 p.
Khoddami A, Wilkes M, Roberts T, Khoddami A, Wilkes MA, Roberts TH. Techniques for analysis of plant phenolic compounds. Molecules. 2013; 18(2):2328–2375.
Trease, Evans W. Pharmacognosy 16th Edition. In: 16th ed. Toronto: Saunders Elsevier; 2009. p. 100, 246, 269–270.
Hayat J, Akodad M, Moumen A, Baghour M, Skalli A, Ezrari S, Belmalha S. Phytochemical screening, polyphenols, flavonoids and tannin content, antioxidant activities and FTIR characterization of Marrubium vulgare L. from 2 different localities of Northeast of Morocco. Heliyon. 2020; 6(e05609).
Prieto P, Pineda M, Aguilar M. Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E. Anal Biochem. 1999; 269:337–341.
Jan S, Khan MR, Rashid U, Bokhari J. Assessment of Antioxidant Potential, Total Phenolics and Flavonoids of Different Solvent Fractions of Monotheca Buxifolia Fruit. Osong Public Heal Res Perspect [Internet]. 2013;4((5)):246–54.
Ahmed D, Khan MM, Saeed R. Comparative Analysis of Phenolics, Flavonoids, and Antioxidant and Antibacterial Potential of Methanolic, Hexanic and Aqueous Extracts from Adiantum caudatum Leaves. Antioxidants. 2015; 4((2)):394–409.
Brindha P, Ragamanvitha A, Narendran R, Sriram S, Vadive V. Antioxidant Activity and Phytochemical Composition of Aqueous Extract of Markhamia lutea(Benth) K. Schum. Leaves. Trop J Nat Prod Res. 2017; 1(2):63–68.
Habisukan U, Elfita, Widjajanti H, Setiawan A, Kurniawati A. Antioxidant and Antimicrobial Activity of Endophytyc Fungi Isolated from Syzygium aqueum Leaves. J Phys Conf Ser ICASMI Sumatera. 2020; 1–8.
Primadiastri IZ, Wulansari ED, Suharsanti R. Perbandingan Kandungan Fenolik Total, Flavonoid Total Aktivitas Antioksidan Ekstrak Etanol Daun Jambu Bol ( Syzigium malaccense L .) Dan Daun Jambu Air Kancing ( Syzigium aqueum ). J Media Farm Indones. 2021; 16(2):1671–1675.
Hikmawanti NPE, Wiyati T, Abdul Muis M, Nurfaizah FA, Septiani W. Total Flavonoids Content of Polar Extracts of Cayratia trifolia Leaves. IOP Conf Ser Earth Environ Sci. 2021; 819(1), 1-4.