Effect of Solvent Extraction on Antityrosinase and Sun Protection Factor of Mulberry (Morus alba L.) Cultivated in Wajo, Indonesia http://www.doi.org/10.26538/tjnpr/v7i6.7
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Abstract
Mulberry (Morus alba L.) is commonly used as a silkworm feed and a medicinal herb in Indonesia. There has not been much research on mulberry leaf pharmacological activities influenced by different planting places, whether because of different soil characteristics or other environmental factors such as ecology. This study aimed to investigate the effect of solvent extraction on antityrosinase and sun protection factor (SPF) of the mulberry leaves grown in Wajo, South Sulawesi, Indonesia. This study separately used different solvent extraction methods (96% ethanol, hexane, ethyl acetate, chloroform, and butanol). Antityrosinase was investigated in vitro assays, while UV spectrophotometry was used to measure the UV absorption, and the Mansur equation was applied to obtain the final SPF. The result showed that 96% ethanol extract presented the most potent to inhibit tyrosinase (IC50 35.03±0.16 µg/mL) followed by butanol (IC50 159.26±2.19 µg/mL), chloroform (IC50 234.51±22.14 µg/mL), ethyl acetate (IC50 283.76±3.65 µg/mL) and hexane (523.97±54.73 µg/mL). Our finding also suggested that butanol extract has the highest SPF value of 12. 84±0. 55 (high protection category) at 750 µg/mL. Overall, the experimental results revealed that mulberry significantly inhibits hyperpigmentation-related
tyrosinase and sun protection, indicating that they might be used as bioactive metabolites in cosmetic and medicinal formulations to combat skin hyperpigmentation.
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