Cardio-Protective Effect of Hunteria umbellata Seed: Experimental and In-silico Approaches doi.org/10.26538/tjnpr/v6i1.16
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Abstract
Cardiotixicity is one of the main causes related to sudden death in the world. The study investigated the ameliorative potential of Hunteria umbellata against isoproterenol (ISO)- induced cardiotoxicity using experimental and in-silico approaches. The ethanol extract of H. umbellata was prepared, phytochemically screened and quantified. In vitro antioxidant potential of the extract was also carried out. Male Wistar rats (30) were randomly and equally grouped into five and used for this study. After administration of the extract, the rats were sacrificed. Thereafter, the cardiac toxicity biomarkers (CK-MB, LDH, AST) and lipid profiles [Triglycerides (TG), Total cholesterol (TC) and High-density cholesterol (HDL-c)] were estimated from the blood plasma. Five molecular compounds from H. umbellata seed were selected based on their percentage yield and they were subjected to optimization using Spartan The optimized compounds were docked against β1-adrenergic receptor (PDB ID: 2ycw). Data obtained was analyzed using standard descriptive and inferential statistics. Pre-treatment with the extract caused a significant decrease in the levels LDH, AST, TC and TG with the most apparent effect at 100 mg/kg bwt. There was no significant difference in the activity of CK-MB. It was observed that compound (A), (C) and (E) inhibited β1-adrenergic receptor than the referenced drug. In conclusion, H. umbellata exhibited ameliorative effects against ISO-induced myocardial damage, suggesting preliminary anti-dyslipidemia abilities possibly due to high phenolic and flavonoid contents.
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