Evaluation of Antioxidant, Antihemolytic Activity, Analgesic and Anti-Inflammatory Potential of Aristolochia clematitis Extracts http://www.doi.org/10.26538/tjnpr/v7i5.23
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Abstract
There is an increasing interest in medicinal plants to find natural remedies relatively safer than synthetic alternatives. The present study reports the evaluation of the in vitro and in vivo biological properties of crude extract (CrE), hexane (HeE), ethyl acetate (EAE) and aqueous (AqE) fractions of Aristolochia clematitis. The polyphenol and flavonoid content were determined and varied from 15.77 ± 0.28 to 93.62 ± 1.63 mg EGA/g, and 5.71 ± 0.1 to 13.53 ± 0.15 µg EQ/mg, respectively. Crude extract (CrE) and ethyl acetate (EaE) showed the highest scavenging activity of DPPH radicals (IC50 = 50.66 ± 1.41 and 69.57 ± 0.38 µg/mL) and hydroxyl radicals (IC50 = 0.894 ± 0.042 and 0.905 ± 0.890 mg/mL), reducing power (113.85 ± 0.5 and 82.45 ± 0.13 mg/mL) and the ability to inhibit β-carotene oxidation (76.13 ± 0.93, 69.18 ± .95 %). All extracts have the ability to protect red blood cells from AAPH peroxyl radicals and significantly increase hemolysis time compared to vitamin C. Tested on normal human plasma at a concentration of 50 and 100 mg/mL, these extract prolonged prothrombin (PT) and partial thromboplastin (aPTT) times. In addition, CrE tested at doses of 150 and 300 mg/kg, showed
potent antinociceptive effect (40.68 ± 3.84 and 66.61 ± 4.71%) and anti-inflammatory activity (65.71 ± 1.41 and 63.89 ± 2.17%). The results support the use of this plant in traditional medicine and suggest that it may contain phytochemicals that have the potential to be active agents as antioxidants, anticoagulants and analgesics.
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References
Anne S. van Wyk, Gerhard Prinsloo. Health, safety and quality concerns of plant-based traditional medicines and herbal remedies. S Afr J Bot. 2020; 133: 54-62.
Batiha GES, Magdy BA, Wasef L, Elewa YHA, Abd ElHack ME, Taha AE, Al-Sagheer AA, Devkota HP, Tufarelli V. Uncaria tomentosa (Willd. ex Schult.) DC: A review on chemical constituents and biological activities. Appl Sci 2020;10:2668.
Blasi F, Cossignani L. An Overview of natural extracts with antioxidant activity for the improvement of the oxidative stability and shelf life of edible oils. Processes. 2020; 8: 956.
Xu X, Liu A, Hu S, Ares I, Martínez-Larrañaga M, Wang X, Martínez M, Anadón A, Martínez MA. Synthetic phenolic antioxidants: Metabolism, hazards and mechanism of action. Food Chem. 2021;1:129488.
Oh TW, Do HJ, Jeon J-H, Kim K. Quercitrin inhibits platelet activation in arterial thrombosis. Phytomedicine. 2021;80:153363.
Ullah A, Munir S, Badshah SL, Khan N, Ghani L, Poulson BG, Emwas A, Jaremko M. Important flavonoids and their role as a therapeutic agent. Molecules. 2020;25: 5243.
Heinrich M, Wanke S, Simmonds MSJ. Local uses of Aristolochia species and content of nephrotoxic aristolochic acid 1 and 2-A global assessment based on bibliographic sources. J Ethnopharmacol. 2009;125:108-144.
Subramaniyan V, Saravanan R, Baskaran D, Ramalalingam S. In vitro free radical scavenging and anticancer potential of Aristolochia indica L. against MCF-7 cell line. Int J Pharm. 2015;7:392-396.
Bahmani M, Eftekhari Z. An ethnoveterinary study of medicinal plants in treatment of diseases and syndromes of herd dog in southern regions of Ilam province, Iran. Comp Clin Path. 2013;22:403-407.
Gupta MP, Solís PN, Calderón AI, Guinneau-Sinclair F, Correa M, Galdames C, Guerra C, Espinosa A, Alvenda GI, Robles G, Ocapo R. Medical ethnobotany of the tribes of Bocas del Toro, Panama. J Ethnopharmacol. 2005;96:389- 401.
Jarić S, Popović Z, Mačukanović-Jocić M, Djurdjević L, Karadžić B, Mijatović M, Pavlović P. An ethnobotanical study on the usage of wild medicinal herbs from Kopaonik Mountain (Central Serbia). J Ethnopharmacol. 2007;111:160-175.
Baghiani A, Boumerfeg S, Adjadj M, Ameni D, Djarmouni M, Khelifi Touhami F, Charef N, Khennouf S, Arrar L. Antioxidants, Free Radicals Scavenging and Xanthine Oxidase Inhibitory Potentials of Ajuga iva L. Extracts. Free Radic Antioxid. 2011;1(4):21-30.
Saffidine K, Sahli F, Zerroug MM. Antioxidant and antimicrobial activities of Plantago major. Int J Pharm Pharm Sci. 2015;7(5):58-64.
Adjadj M, Djarmouni M, Baghiani A. Xanthine oxidase inhibitory activity and antioxidant property of various extracts from the medicinal plant Paronychia argentea L. AJB2T. 2017;2(3):1-9.
Aouachria S, Boumerfeg S, Benslama A, Benbacha F, Guemmez T, Khennouf S, Arrar L, Baghiani A. Acute, sub-acute toxicity and antioxidant activities (in vitro and in vivo) of Reichardia picroide crude extract. J Ethnopharmacol. 2017 17;208:105-116.
Saffidine K, Fatima Z, Guemmaz T, Lameche C, Sahli F and Baghiani A. Antioxidant, Antibacterial Activities and Synergistic Effect with Antibiotics of Fruits and Stems Extracts from Styphnolobium japonicum (L.) Schott. TURJAF. 2022; 10(4): 760-765.
Guemmaz T, Zerargui F, Boumerfeg S, Arrar L, Aouachria S, Khennouf S, Charef N, Baghiani A. Anti-hemolytic, antilipid peroxidation, antioxidant properties and acute toxicity of Xanthium strumarium Leaves Extracts. Ann Res Rev Biol. 2018;24(3):1-12.
Qi X, Mao W, Chen Y, Chen Y, Zhao C, Li N, et al. (2012. Chemical characteristics of an anticoagulant active sulfated polysaccharide from Enteromorpha clathrata. Carbohydpolym. 2012;90(4):1804-1810.
Ahmed S, Naved A, Khan RA, Siddiqui S. Analgesic Activities of Methanol Extract of Terminalia chebula Fruit. Pharm Pharmacol. 2015;6(12):547-553.
Kou J, Ni Y, Li N, Wang J, Liu L, Jiang Z-H. Analgesic and Anti-inflammatory Activities of Total Extract and Individual Fractions of Chinese Medicinal Ants Polyrhachis lamellidens. Biol Pharm Bull. 2005; 8(1):176-180.
Benmehdi H, Behlil A, Mennou F, Amrouche A. Free radical scavenging activity, kinetic behaviour and phytochemical constituents of Aristolochia clematitis L. roots. Arab J chem. 2017;10:S1402-S1408.
Merouani N., Belhattab R. and Sahli F. Evaluation of the biological activity of Aristolochia longa L. extracts. Int j Pharm Sci Res. 2017;8(5):1978-1992.
Ngozi P Okolie, Falodun A and Oluseyi Davids. Evaluation of the antioxidant activity of root extract of pepper fruit (Dennetia tripetala), and its potential for the inhibition of Lipid peroxidation. Afri J Trad Complem and Altern Med. 2014;11(3):221-227.
Martemucci M, Costagliola C, Mariano, D’andrea L, Napolitano P, D’Alessandro AG. Free Radical properties, source and targets, antioxidant consumption and health. Oxygen. 2022;2:48-78.
Liao W, Ning Z, Chen L, Wei Q, Yuan E, Yang J, Ren J. Intracellular antioxidant detoxifying effects of diosmetin on 2,2-azobis(2-amidinopropane) dihydrochloride (aaph)- induced oxidative stress through inhibition of reactive oxygen species generation. J Agric Food Chem. 2014;62:8648-8654.
Egharevba E, Chukwuemeke-Nwani P, Eboh U, Okoye E, Bolanle IO, Oseghale IO, Imieje VO, Erharuyi O, Falodun A. Antioxidant and Hypoglycaemic Potentials of the Leaf Extracts of Stachytarphyta jamaicensis (Verbenaceae). Trop J Nat Prod Res. 2019; 3(5):170-174.
Li M, Pare WP, Zhang J, Kang T, Zhang Z, Yang D, Wang K, Xing H. Antioxidant Capacity Connection with phenolic and flavonoid content in chinese medicinal herbs. Rec Nat Prod. 2018;12(3):239-250.
Muniyandi K, George E, Sathyanarayanan S, George BP, Abrahamse H, Thamburaj S. Thangurage, P. Phenolics, tannins, flavonoids and anthocyanins contents influenced antioxidant and anticancer activities of Rubus fruits from Western Ghats, India. Food Sci Hum Wellness. 2019;8:73- 81.
Hmidani A, Tariq Bouhlali ED, Khouya T, Ramchounbd M, Filali-Zegzoutia Y, Alemb C, Benlyasa M. antioxidant,antiinflammatory and anticoagulant activities of three Thymus species grown in southeastern Morocco. FJPS. 2019;5:4.
Skalski B, Pawelec P, Jedrejek D, Rolnik A, Pietukhov R, Piwowarczyk R, Stochmal A, Olas B. Antioxidant andanticoagulant effects of phenylpropanoid glycosides isolated from broomrapes (Orobanche caryophyllacea, Phelipanche arenaria, and P. ramosa). Biomed
Pharmacother. 2021;139:111618.
Abubakar A, BalarabeNazifi A, Odoma S, Shehu S, Danjuma NM. Antinociceptive activity of methanol extract of Chlorophytum alismifolium tubers in murine model of pain: Possible involvement of a2-adrenergic receptor and K ATP channels. J Tradit Complement Med. 2020;10:1-6.