Comparison of Three Different Extraction Methods for the Determination of the Quality of the Leaf Volatile Oils of Delonix regia (Bojer ex Hook.Raf).

doi.org/10.26538/tjnpr/v6i1.13

Authors

  • Isaac S. Njoku Department of Chemistry, Faculty of Basic Medical and Applied Sciences, Trinity University Yaba, Lagos, Nigeria
  • Nisar-Ur Rahman Department of Pharmacy,COMSATS University Islamabad, Abbottabad Campus, Pakistan.
  • Ahsan M. Khan Department of Pharmacy,COMSATS University Islamabad, Abbottabad Campus, Pakistan.
  • Idowu Otunomo Department of Pure and Industrial Chemistry, Faculty of Science,University of Nigeria, Nsuka, Enugu State, Nigeria.
  • Olayinka T. Asekun Department of Chemistry, Faculty of Science, University of Lagos, Akoka-Yaba, Lagos, Nigeria
  • Oluwole B. Familoni Department of Chemistry, Faculty of Science, University of Lagos, Akoka-Yaba, Lagos, Nigeria
  • Chibuko Ngozi Department of Fisheries, Faculty of Science, University of Lagos, Akoka-Yaba, Lagos, Nigeria

Keywords:

Delonix regia, Extraction, Caryophyllene, Thymol, Volatile oil

Abstract

Essential oils from natural sources form a new range of potent antimicrobial and antioxidant agents, hence, there is the need to explore the best method for their extraction, which retains the bioactive components of these oils. This research studied the effect of three different extraction methods on the yield, chemical composition, hence, quality of the volatile oils from the leaves of Delonix regia. Three different methods (hydrodistillation, steam distillation and cold maceration) were employed in the extraction of the oils from the leaves of Delonix regia plant. The extracted oils were subjected to Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The yield of oils obtained from the hydrodistilled, steam distilled and macerated air dried leaves were 0.32%, 0.54% and 1.25%, respectively. A total of 22, 22 and 12 compounds were identified using Gas chromatography in the hydrodistilled, steam distilled and macerated air-dried plant materials, respectively. Maceration of plant in n-hexane gave the  highest oil yield (1.25%) compared to hydrodistillation (0.32%) and steam distillation (0.54%). The volatile oil of Delonix regia was composed mainly of monoterpenoids and sesquiterpenoids. The various extraction methods gave oils with varying major constituents- hydrodistillation, thymol (40.09%), steam distillation, caryophyllene (41.64%) and Cold maceration, Benzene (1,1- dimethylethoxy) (61.90%). The result revealed that the different extraction methods affected the yield, chemical composition and hence, the quality of the volatile oils of Delonix regia

Author Biography

Isaac S. Njoku, Department of Chemistry, Faculty of Basic Medical and Applied Sciences, Trinity University Yaba, Lagos, Nigeria

Department of Chemistry, Faculty of Science, University of Lagos, Akoka-Yaba, Lagos, Nigeria

Department of Pharmacy,COMSATS University Islamabad, Abbottabad Campus, Pakistan.

References

Jain S, Vaidya A, Jain N, Kumar V, Modi A. Bioactive Compounds of Royal Poinciana (Delonix regia (Hook.) Raf.). Bioactive Compounds in Underutilized Vegetables and Legumes. 2021; 483-502p.

Algharib AH, Abd El Hakim AF, El-Khamissi HA, ElHamamsy SM. Possibility of Using Golden Shower (Cassia Fistula) and Poinciana (Delonix regia) Seeds Oil as NonConventional Feedstocks for the Production of Biodiesel in Egypt. J Ecol Eng. 2021; 22(10):19-27.

Abdou MAH, Raouf AE, Reem M, Aboel-Ainin MA, Abdel Gaber AF, Hassan AA. Effect of some of organic and bio and/or mineral fertilization on vegetative growth and chemical composition of Delonix regia. Sci J Flowers Ornam Plants. 2020; 7(4):527-539.

Kumawat RB and Sh RA. Ethnophormacological screening. Res J Rec Sci. 2017; 6(5):32-41.

Salem MZM. Evaluation of the antibacterial and antioxidant activities of stem bark extracts of Delonix regia andErythrina humeana grown in Egypt. J Forest Prod Ind. 2013; 2(2):48-52.

Ramakrishnan RB. Evaluation of antioxidant and phytochemical activity in solvent extracts from Delonix regia flowers. Int J Green Pharm. 2018; 12(03):607-616.

Chen BM, Liao HX, Chen WB, Wei HJ, Peng SL. Role of allelopathy in plant invasion and control of invasive plants. Allelopathy J. 2017; 41:155-166.

Dhawan SR, Dhawan P, Gupta SK. Allelopathic potential of leguminous plant species towards Parthenium hysterophorus L. Flora and Fauna (Jhansi). 1998; 4(1):9-12.

Dhawan SR, Dhawan P, GuptaSK. Allelopathic potential of leguminous plant species towards Parthenium hysterophorus L.(1)-effect of aqueous foliar leachates. Legume Res. 2001; 24(4):256-259.

Adewuyi A, Rotimi A, Oderinde B, Rao VSK, Prasad RBN, Anjaneyulu B. "Chemical component and fatty acid distribution of Delonix regia and Peltophorum pterocarpumseed oils." Food Sci Technol R. 2010; 16(6):565-570.

Goldson Barnaby A, Reid R, Rattray V, Williams R, Denny M. "Characterization of Jamaican Delonix regia and Cassia fistula seed extracts." Biochem Res Int. 2016; :1-8.

Enikuomehin OA and Kehinde IA. In vitro screening of some tropical ash samples against seedborne pathogens of wheat (Triticum aestivum L.). Australas. Plant Pathol. 2007; 36(6):587-590.

Njoku IS, Ichide MU, Rahman N, Khan AM, Chibuko NA, Asekun OT, Familoni OB. Extraction, Characterization and Larvicidal Activity of Essential Oil and Hydrosol from Sida acuta Burm. f. Leaves Grown in Nigeria. Trop J Nat Prod Res. 2021; 5(1):211-216.

Koenig WA, Hochmuth DH, Joulain D. Mass Finder 2.1 (Including the library) of terpenoids and related constituents of essential oils. E-B Verlag: Hamburg.1998.

Njoku IS, Ichide MU, Rahman N, Khan AM, Otunomo I, Asekun, OT, Familoni OB. Variation In the Sesquiternoid Composition of the Volatile Oils of Annona muricata Linn. from South-Western Nigeria, Caused by Different Drying Methods. J Chem Soc Nig. 2021; 46(4):0765 – 0771

http://webbook.nist.gov/chemistry/ (accessed 23 January, 2020)

El-Sayed AM. The Pherobase: Database of pheromones and semiochemicals. 2014. URL http://www. pherobase. Com.2012. (accessed 15 June, 2021)

Adams RP. Identification of essential oil components by gas chromatography/mass spectrometry. Vol. 456. Carol Stream, IL: Allured publishing corporation, 2007.pp.804.

Sefidkon F, Khadijeh A, Gholamreza BK. Influence of drying and extraction methods on yield and chemical composition of the essential oil of Satureja hortensis. Food Chem. 2006; 99(1):19-23.

Rajeswara Rao BR, Syamasundar KV, Patel RP. "Effect of method of distillation on the yield and chemical composition of Artemisia annua essential oil." J Essent Oil Res. 2014; 26(6):486-491.

Njoku IO, Asekun OT, Familoni OB. Effects of Different Drying Methods on the Chemical Composition of the Essential Oils of Ocimum gratissimum L. Leaves. Nig J Pure Appl Sci. 2017; 30(3):3109-3115.

Salem MZ, Abdel-Megeed A, Ali HM. Stem wood and bark extracts of Delonix regia (Boj. Ex. Hook): Chemical analysis and antibacterial, antifungal, and antioxidant properties. BioResourc. 2014; 9(2): 2382-2395.

Sousa OV, Del-Vechio-Vieira G, Alves MS, Araújo AA, Pinto MA, Amaral MP, Rodarte MP, Kaplan MA. Chemical composition and biological activities of the essential oils from Duguetia lanceolata St. Hil. barks. Molecules. 2012; 17(9):11056-11066.

Lo Presti M, Ragusa S, Trozzi A, Dugo P, Visinoni F, Fazio A, Dugo G, Mondello L.A comparison between different techniques for the isolation of rosemary essential oil. J Sep Sci. 2005; 28(3):273-280.

Goodner KL, Kanjana M, Anne P, Sotomayor JA, Jordan MJ. Aromatic profiles of Thymus hyemalis and Spanish T. vulgaris essential oils by GC–MS/GC–O. Ind Crop Prod. 2006; 24(3):264-268.

Novy P, Davidova H., Serrano-Rojero C.S, Rondevaldova J, Pulkrabek J. and Kokoska L, Composition and antimicrobial activity of Euphrasia rostkoviana Hayne essential oil. Evid-Based Compl Altern Med. 2015; ():1-5.

"Thymol" (PDF). US Environmental Protection Agency. September 1993. Technical Document for 5-methyl-2-isopropyl-1-phenol ...https://www3.epa.gov › reactions › registration. (Accessed 15 June, 2021).

Klauke AL, Racz I, Pradier B, Markert A, Zimmer AM, Gertsch J, Zimmer A. "The cannabinoid CB2 receptor-selective phytocannabinoid beta-caryophyllene exerts analgesic effects in mouse models of inflammatory and neuropathic pain." Eur Neuropsychopharmacol. 2014; 24(4):608-620.

Bahi A, Al Mansouri S, Al Memari E, Al Ameri M, Nurulain SM, Ojha S. β-Caryophyllene, a CB2 receptor agonist produces multiple behavioral changes relevant to anxiety and depression in mice. Physiol behav. 2014; 135:119-124.

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Published

2022-01-01

How to Cite

S. Njoku, I., Rahman, N.-U., M. Khan, A., Otunomo, I., T. Asekun, O., B. Familoni, O., & Ngozi, C. (2022). Comparison of Three Different Extraction Methods for the Determination of the Quality of the Leaf Volatile Oils of Delonix regia (Bojer ex Hook.Raf).: doi.org/10.26538/tjnpr/v6i1.13. Tropical Journal of Natural Product Research (TJNPR), 6(1), 71–75. Retrieved from https://tjnpr.org/index.php/home/article/view/197

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