Screening of Antibacterial Compounds Against Escherichia coli from Hanjeli Seeds (Coix lacryma-jobi) Based on Metabolomics http://www.doi.org/10.26538/tjnpr/v7i5.4
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Abstract
Hanjeli seed (Coix lacryma-jobi L.) is one of the important plants used as raw materials for food and traditional medicine. In addition, hanjeli seed also has several biological activities, one of which is antibacterial activity comes from the activity of the metabolites contained in the extract of the seed. This study aims to determine the antibacterial activity of hanjeli seed extract and identify the metabolites contained in some selected extracts using a metabolomics approach. Hanjeli seed was extracted with distilled water, 50% ethanol, 96% ethanol, and n-hexane. The crude extracts of hanjeli seed were evaluated for the activity against Escherichia coli ATCC 25922 and the metabolites were identified using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). Hanjeli seed extracts had antibacterial activity ranging from 7.85±4.48 to 27.99±6.02%, with 50% ethanol extract had the highest antibacterial activity and nhexane extract had the lowest. LC-MS/MS analysis of the 50% ethanol extract obtained 22 metabolites which were putatively identified. The antibacterial activity of the hanjeli seed extract is predicted to be caused by the phenolic compounds, such as gluconic acid, quinic acid, malic acid, citric acid, p-coumaric acid, and ferulic acid. The phenolic compounds contained in the hanjeli seed extract can inhibit the growth of E. coli, so that the extract can be applied as an antibacterial against E. coli.
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