Upregulation of Interleukin-33, Human Β-Defensin 2, and Toll-Like Receptor 2 in Response to Staphylococcus aureus Furunculated Patients http://www.doi.org/10.26538/tjnpr/v7i5.2
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Abstract
A furuncle is an infection of the skin and subcutaneous tissue that involves hair follicles and ultimately results in the formation of an abscess. Furunculosis is most frequently caused by Staphylococcus aureus. interleukin-33, human β defensin 2, and Toll-like receptor 2 (Toll like receptor 2) play a significant role in the innate immunity of the host. The present study aims to determine the level of the interleukin-33, human β defensin 2, and Toll like receptor 2 gene expression in staphylococcal furunculosis. A case-control study includes 50 patients and 50 control were evaluated for the level of interleukin 33, human β defensin and Toll like receptor 2 by enzyme and qPCR, respectively. The results indicated that furunculosis was most prevalent in people between the ages of 25 and 45 (P > 0.05). However, there was no significant difference between males and females (P > 0.05). The serum levels of interleukin-33 were observed to be significantly higher in FP compared to HC. Toll like receptor 2 and human β defensin 2 were elevated in PF, but much more in female patients than in male patients (P < 0.05). In conclusion, the findings of the present study reveal that it is more likely that Toll like receptor 2, human β defensin 2, and interleukin-33 play a significant role in the host's defense against S. aureus infection.
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References
van Belkum A, Verkaik NJ, de Vogel CP, Boelens HA, Verveer J, Nouwen JL, Verbrugh HA, Wertheim HF. Reclassification of Staphylococcus aureus nasal carriage types. J Infect Dis. Jun 15 2009;199(12):1820-1826.
Wertheim HFL, Melles DC, Vos MC, van Leeuwen W, van Belkum A, Verbrugh HA, Nouwen JL. The role of nasal carriage in Staphylococcus aureus infections. Lancet Infect Dis . 2005;5(12):751-762.
Ibler KS, Kromann CB. Recurrent furunculosis - challenges and management: a review. Clin Cosmet Investig Dermatol. 2014;7:59-64.
Nowicka D, Grywalska E. Staphylococcus aureus and Host Immunity in Recurrent Furunculosis. Dermatol. 2019;235:295-305.
Durupt F, Mayor L, Bes M, Reverdy ME, Vandenesch F, Thomas L, Etienne J. Prevalence of Staphylococcus aureus toxins and nasal carriage in furuncles and impetigo. Br J Dermatol. 2007;157(6):1161-1167.
Gong Z, Zhang J, Zhang S, Cao J, Fu Y, Hu X, Zhao J, Gu B, Li Q, Zhang K, Ren P, Liu B, Mao W. TLR2, TLR4, and NLRP3 mediated the balance between host immune-driven resistance and tolerance in Staphylococcus aureus-infected mice. Microb Pathog. Aug 2022;169:105671.
Kielian T, Esen N, Bearden ED. Toll-like receptor 2 (TLR2) is pivotal for recognition of S. aureus peptidoglycan but not intact bacteria by microglia. Glia. Mar 2005;49(4):567-576.
Schröder J-M, Harder J. Human beta-defensin-2. Int J Biochem Cell Biol . 1999;31(6):645-651.
Wang G. Human antimicrobial peptides and proteins. Pharmaceuticals (Basel). May 13 2014;7(5):545-594.
Dumitru CA, Hemeda H, Jakob M, Lang S, Brandau S. Stimulation of mesenchymal stromal cells (MSCs) via TLR3 reveals a novel mechanism of autocrine priming. FASEB J. Sep 2014;28(9):3856-3866.
Sun L, Young L, Zhen Q, Inventors. Application of CaMK4 in Preparation of Medicine for Preventing and Treating psoriasis2022.
Fu J, Bian L, Zhao L, Dong Z, Gao X, Luan H, Sun Y, Song H. Identification of genes for normalization of quantitative real-time PCR data in ovarian tissues. Acta Biochim Biophys Sin (Shanghai). Aug 2010;42(8):568-574.
Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(- Delta Delta C(T)) Method. Methods. Dec 2001;25(4):402- 408.
Paller A, Mancini A. Bacterial, Mycobacterial, and Protozoal Infections of the Skin. In: Paller A, Mancini A, eds. Hurwitz Clinical Pediatric Dermatology. 5th ed: Elsevier; 2016.
Demos M, McLeod M, Nouri K. Recurrent furunculosis: a review of the literature. Br J Dermatol .2012; 167:725 –732.
Narain U, Bajaj AK, Kant A. Recurrent Furunculosis: incidence of anaerobes and fungi. IJAM. 2017;4:1002-1004.
Yin H, Li X, Hu S, Liu T, Yuan B. L-33 promotes Staphylococcus aureus-infected wound healing in mice. Int Immunopharmacol. 2013;17:432–438.
Li C, Li H, Jiang Z, Zhang T, Wang Y, Li Z, Wu Y, Ji S, Xiao S, Ryffel B, Radek K, Xia Z, Lai Y. Interleukin-33 Increases Antibacterial Defense by Activation of InducibleNitric Oxide Synthase in Skin. . PLOS One. 2014;1003918
Scudiero O BM, Mennitti C, Laneri S, Lombardo B, De Biasi MG, De Gregorio E, Pagliuca C, Colicchio R, Salvatore P, Pero R. . Human Defensins: A Novel Approach in the Fight against Skin Colonizing Staphylococcus aureus. Antibiotics. 2020;9:198.
Lai Y, Cogen AL, Radek KA, Park HJ, Macleod DT, Leichtle A, Ryan AF, Di Nardo A, Gallo RL. Activation of TLR2 by a small molecule produced by Staphylococcus epidermidis increases antimicrobial defense against bacterial skin infections. J Invest Dermatol. 2010;130:2211-2221.
Bernard JJ, Gallo RL. Protecting the boundary: the sentinel role of host defense peptides in the skin. Cell. Mol. Life Sci. 2011;68:2189–2199.
Hashim ST, Fakhry SS, Rasoul LM, Saleh TH, Alrubaii BAL. Genotyping toxins of Clostridium perfringens strains of rabbit and other animal origins. Trop J Nat Prod Res. 2021; 5(4):613–616.
Allami RH, Hassoon AH, Abdulateef YM, Ghani AA. Genetic Association of Angiotensin-converting enzyme 2 ACE-2 (rs2285666) Polymorphism with the Susceptibility of COVID-19 Disease in Iraqi Patients. Trop J Nat Prod Res. 2023;7(2):2346-2351
Al-Musawi, AHO, Aziz, HM, Khudair, S, Saleh, TH. Molecular characterization of HBB gene mutations in betathalassemia patients of Southern Iraq. Biomedicine. 2022; 42(5):1040-1043.
Abdulrazaq RA., Mahmood WS, Alwan B, Saleh TH, Hashim ST, Al-Rubaii BAL. Biological study of protease produced by clinical isolates of Staphylococcus aureus. Res J Pharm Technol.2022; 15(12):5415-5420.
Rasoul LM, Allami RH, Alshibib AL, laftaah Al-Rubaii BA, Sale TH. Expression and cytotoxic effect of recombinant Newcastle Disease Virus (rNDV) vector expressing enhanced green fluorescent gene in JHH5 cell line. Biomedicine. 2023; 43(1):205-209.
Jawad NK, Numan AT, Ahmed AG, Saleh TH, Al-Rubaii BA. IL-38 gene expression: A new player in Graves’ ophthalmopathy patients in Iraq. Biomedicine. 2023;43(1):210-215.
Eyad HN, Adbulateed YA, Lafi SA. Abnormal presentation of TB patients: anthropological study, Ann Trop Med and Public Health. 2019; 22(VI): S186.
Bresam S, Al-Jumaily RM, Karim GF, Al-Rubaii BA. Polymorphism in SNP rs972283 of the KLF14 gene and genetic disposition to peptic ulcer. Biomedicine. 2023;43(1):216-220.
Rasoul LM., Marhoon AA, Albaayit SFA, Ali RW, Saleh,TH, Al-Rubaii BAL. Cytotoxic effect of cloned EGFP gene on NCI-H727 cell line via genetically
engineered gene transfer system. Biomedicine (India). 2022, 42(5): 938-942.
Abbas MS, Ahmed AG, Ali SQ, AL-Rubaii BA. Immunological inflammatory factors in patients diagnosed with COVID-19. Biomedicine. 2023; 43(1):230-235.
Rasin KH, Algabar FA, Al-Obaidi M, Krayfa AH. Evalution of medical waste management during the COVID-19 in Diyala Governorate . Environmental Engineering & Management Journal. 2022 ;21(12):1931–1944.
Abdulateef Y. Role of PSA in Diagnosis of Chronic Prostatitis. Indian Journal of Forensic Medicine & Toxicology. 2020;14(1):774-779.