Phytochemical Analysis and Antioxidant Evaluation of Crude Extracts from the Roots, Stem and Leaves of Dictyandraarborescens (Welw.)


  • Uchechi E. Enenebeaku Department of Biotechnology, Federal University of Technology, Owerri, Nigeria
  • Chidi E. Duru Department of Chemistry, Imo State University, Owerri, Nigeria
  • Evangeline N. Okotcha Department of Integrated Science, Federal College of Education (Technical) Asaba, Nigeria
  • Odangowei I. Ogidi Department of Biochemistry, Federal Polytechnic, Ekowe, Bayelsa State, Nigeria
  • Ifeyinwa C. Mgbemena Department of Biotechnology, Federal University of Technology, Owerri, Nigeria
  • Harriet C. Nwigwe Department of Biotechnology, Federal University of Technology, Owerri, Nigeria
  • Conrad K. Enenebeaku Department of Chemistry, Federal University of Technology Owerri, Nigeria


Dictyandra arborescens, Gas-chromatography, Phytochemical, Antioxidants


Antioxidants are compounds which play important roles in the prevention of many diseases by

removing free radical intermediates and inhibit other oxidation reactions by being oxidized

themselves. Dictyandra arborescens, a tropical plant, is locally used by natives of Mbaise, Imo

State, Nigeria for the treatment of various ailments. Phytochemical analysis and in vitro

antioxidant activities of parts of the plant were evaluated. Gas chromatography flame-ionization

detector (GC-FID) was used to determine the phytochemicals present in the leaves and roots

while in vitro antioxidant potentials of extracts of the leaves, stem and roots were determined by

their abilities to scavenge 2, 2-diphenyl-1- picrylhydrazyl (DPPH), nitric oxide (NO), and

hydrogen peroxide radicals (H2O2). Ferric reducing antioxidant power (FRAP) and total

antioxidant capacity were also determined using standard methods. GC-FID analysis of the leaves and roots identified phytochemicals such as; rutin, ribalinidine, naringin, flavan-3-ol, epihedrine, sapogenin, spartein, flavonones, epicatechin, phytate, oxalate, naringenin, resveratol, quinine, catechin, lunamarin, kaempferol etc. Scavenging of the extracts were concentration dependent. Activities of the extracts for DPPH, nitric oxide and H2O2 differed significantly (p˂0.05) from the standard antioxidant. Methanolic extract of D. arborescens roots showed the best reducing ability while methanolic extract of D. arborescensroot also showed the highest antioxidant activity (2352.94 ±164.87 mg AA/g extract). Findings showed that extracts of the leaves, and roots of D. arborescens were rich in phytoconstituents which could serve as potential sources of natural antioxidants. Antioxidant and therapeutic activities of this plant could therefore be linked to the synergy between the various phytochemicals.  


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How to Cite

E. Enenebeaku, U., E. Duru, C., N. Okotcha, E., I. Ogidi, O., C. Mgbemena, I., C. Nwigwe, H., & K. Enenebeaku, C. (2022). Phytochemical Analysis and Antioxidant Evaluation of Crude Extracts from the Roots, Stem and Leaves of Dictyandraarborescens (Welw.): Tropical Journal of Natural Product Research (TJNPR), 6(1), 62–70. Retrieved from