Beta-D-glucan Polysaccharide Downregulates p53, and Prostate Specific Antigen Expression in Histological and Immunohistochemical study of Prostate Tumor Model http://www.doi.org/10.26538/tjnpr/v7i4.23
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Global increase in cancer incidence has prompted the search for natural bioactive products which are readily available, safer and more affordable. Beta-D-glucan polysaccharide (βDgP) is a phytochemical fractionate of an edible mushroom Auricularia polytricha known to have anti cancer property but information about the actual mechanism underlying its therapeutic property is lacking. This research investigates the tumor regulatory effect of Beta-D-glucan polysaccharide on some tumor markers; p53 and Prostate Specific Antigen (PSA) in nitrosobis amine (NA) – induced prostate tumor model of Wister rat. Twenty-four male Wister rats were divided into four groups with six rats in each group. Group A served as normal control while treatment groups: B (5mg/kgbw nitrosobis amine only); C and D (placed on 120 mg/kgbw and 250 mg/kgbw respectively) after inducing tumor. The experiment lasted for 10 weeks. At termination, ELISA method was used to evaluate PSA levels; p53 tumor marker was assessed using immunohistochemical methods; histological alterations were examined using routine H&E technique. Results shown that p53 and PSA levels increased significantly (at p≤0.05) in group B (tumor control) when compared to normal control. Increase in papillary fronds, basal cell hyperplasia and prostatic concretions depicted cytoarchitectural alterations in tumor control group. However, expression of p53 and PSA was down-regulated significantly (p≤0.05) when compared to tumor control, and histological distortions reversed in a dose-dependent manner, following administration of graded concentration of βDgP. Findings from the present study have revealed the anti-tumor property of βDgP in NA-induced prostate tumor model in Wister rat.
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Theresia E, Malueka RG, Pranacipta S, Kameswari B, Dananjoyo K, Asmedi A, Wicaksono AS, Hartanto RA, Dwianingsih EK. Association between Ki-67 Labeling index and Histopathological Grading of Glioma in Indonesian Population. Asian Pac J Cancer Prev. 2020; 21(4):1063-
Campbell FC, Collett PG. Chemopreventive Property of Curcumin. Future Oncol, 2005; 1:405-414
Fukushima H, Masuda H, Kawakami S, Ito M, Sakura M, Numao N, Koga F, Saito K, Fujii Y, Yamamoto S, Yonese J, Fukui I, Kihara K. Effect of Diabetes Mellitus on High-grade Prostate Cancer Detection Among Japanese Obese Patients with Prostate-Specific Antigen Less Than 10 ng/mL. Urology. 2012 Jun;79(6):1329-34. doi: 10.1016/j.urology.2012.01.070. PMID: 22656412.
Rukayat AI, Akeem AO, Fatimo AS, Abubakar I, Emmanuel Y, Olushola OO, Salihu MA. Exogenous Melatonin Restored the Cyto-Architectural Integrity and Biochemical Activities of the Cerebrum in Sodium Fluoride Induced Toxicity. Eur J Anat, 2021; 25 (5): 533-540
Zawacka-Pankau JE. The Undervalued Avenue to Reinstate Tumor Suppressor Functionality of the p53 Protein Family for Improved Cancer Therapy-Drug Repurposing. Cancers, 2020; 12, 2717. DOI: 10.3390/cancers12092717
Peuget S, Selivanova G. p53-Dependent Repression: DREAM or Reality? Cancers 2021, 13, 4850. DOI: 10.3390/cancers13194850
Bromley D, Daggett V. Tumorigenic P53 Mutants Undergo Common Structural Disruptions Including Conversion to Α-Sheet Structure. Protein Sci Publ Protein Soc. 2020; 29:1983–99. doi: 10.1002/pro.3921
Pairawan S, Zhao M, Yuca E, Annis A, Evans K, Sutton D, Carvajal L, Ren J-G, Santiago S, Guerlavais V. First in Class Dual MDM2/MDMX Inhibitor ALRN-6924 Enhances Antitumor Efficacy of Chemotherapy in TP53 Wild-Type Hormone Receptor-Positive Breast Cancer Models. Breast Cancer Res., 2021; 4;23(1):29. doi: 10.1186/s13058-021-01406-x
Sabapathy K, Lane DP. Therapeutic Targeting of P53: All Mutants are Equal, But Some Mutants are More Equal Than Others. Nat Rev Clin Oncol. 2018; 15:13–30. 6. doi: 10.1038/nrclinonc.2017.151
Olivier M, Hollstein M, Hainaut P. TP53 Mutations in Human Cancers: Origins, Consequences, and Clinical Use. Cold Spring Harb Perspect Biol. 2010; 2(1): a001008. DOI: 10.1101/cshperspect.a001008.
Cunningham D, You Z. In Vitro and In Vivo Model Systems Used in Prostate Cancer Research. J Biol Methods. 2015; 2(1):e17. DOI: 10.14440/jbm.2015.63
Agbor CA, Fischer CE, Agaba EA, Nnenna WA. Neuroprotective Effect of Beta-D-glucan Polysaccharide Fractionate of Auricularia Polytrichaon HyperglycaemiaInduced Cerebral Injury in Diabetic Animal Model. Trop J Nat Prod Res, 2021; 5(12):2182-2186. doi.org/10.26538/tjnpr/v5i12.24
Liu X, Wang R, Bi, J, Kang L, Zhou J, Duan B, Liu Z, Yuan S. A Novel Endo-Β-1,6-Glucanase from The Mushroom Coprinopsis Cinerea and Its Application in Studying of Cross-Linking of Β-1,6-Glucan and the Wall Extensibility In Stipe Cell Walls. Int. J. Biol. Macromol. 2020; 160, 612–622.
Morales D, Rutckeviski R, Villalva M, Abreu H, Soler-Rivas C, Santoyo S, Iacomini M, Smiderle FR. Isolation and Comparison of α- and β-d-Glucans from Shiitake Mushrooms (Lentinula edodes) with Different Biological Activities. Carbohydr. Polym., 2020; 229: 115521. https://doi.org/10.1016/j.carbpol.2019.115521
Baeva, E, Bleha, R, Lavrova E, Sushytskyi L, Cop ˇ íková J, Jablonsky I, Klouˇcek P, Synytsya A. Polysaccharides from Basidiocarps of Cultivating Mushroom Pleurotus ostreatus: Isolation and Structural Characterization. Molecules, 2019; 24, 2740. doi: 10.3390/molecules24152740
Agbor CA, Fischer CE, Agaba EA, Nnenna WA. Neuroprotective Effect of Beta-D-Glucan Polysaccharide on Hyperglycaemia-Induced Cerebral Injury in Diabetic Animal Model. Eur J Anat, 2022; 26(2):217–224
Zhang Y, Liu Y, Zhou Y, Zheng Z, Tang W, Song M, Wang J, Wang K. Lentinan Inhibited Colon Cancer Growth by Inducing Endoplasmic Reticulum Stress-Mediated Autophagic Cell Death and Apoptosis. Carbohydr. Polym. 2021; 267, 118154. DOI: 10.1016/j.carbpol.2021.118154
Anyanwu GE, and Agbor CA. Assessment of Testicular Histomorphometric Parameters and Reticular Fibre Density on Testicular Tissue of Diabetic Wister Rat Placed on Auricularia Polytricha. Jordan J. Biol. Sci. 2020; 13(Supplementary): 709-714
Wu X, Zheng Z, Guo T, Wang K, Zhang Y. Molecular Dynamics Simulation of Lentinan and Its Interaction with the Innate Receptor Dectin-1. Int. J. Biol. Macromol., 2021; 171: 527–538. DOI: 10.1016/j.ijbiomac.2021.01.032
Pan W, Jiang P, Zhao J, Shi H, Zhang P, Yang X, Biazik J, Hu M, Hua H, Ge X. β-Glucan from Lentinula Edodes Prevents Cognitive Impairments in High-Fat Diet-Induced Obese Mice: Involvement of Colon-Brain Axis. J. Transl. Med., 2021; 19:54. doi: 10.1186/s12967-021-02724-6
Thongsiri C, Nagai-Yoshioka Y, Yamasaki R, Adachi Y, Usui M, Nakashima K, Nishihara T, Ariyoshi W. Schizophyllum Commune Β-Glucan: Effect on Interleukin-10 Expression Induced by Lipopolysaccharide from Periodontopathic Bacteria. Carbohydr. Polym., 2021; 253: 117285. doi: 10.3390/nu13113960
Ina K, Kataoka T, Ando T. The Use of Lentinan for Treating Gastric Cancer. Anticancer Agents Med. Chem., 2013; 13:681–688. DOI: 10.2174/1871520611313050002
Li LT, Jiang G, Chen Q, Zheng JN. Ki67 is A Promising Molecular Target in the Diagnosis of Cancer (Review). Mol Med Rep. 2015;11(3):1566-72. doi: 10.3892/mmr.2014.2914.
Zi Y, Jiang B, He C, Liu L. Lentinan Inhibits Oxidative Stress and Inflammatory Cytokine Production Induced By Benzo(A)Pyrene in Human Keratinocytes. J. Cosmet. Dermatol., 2020; 19:502–507. DOI: 10.1111/jocd.13005