Antioxidant and Ameliorative Effects of Basella alba L. On Letrozole-Induced Polycystic Ovarian Syndrome in Rats http://www.doi.org/10.26538/tjnpr/v7i4.25
Main Article Content
Basella alba L. (BA) is utilized in Nigeria to manage Polycystic Ovarian Syndrome (PCOS), according to an ethnobotanical study. However, this claim has not been supported by any scientific data. This study investigated the effect of the methanol leaf extract of the plant in alleviating polycystic ovary conditions in rats. The methanol extract of BA was subjected to a brine shrimp lethality assay. Total Phenolic Content (TPC), Total Flavonoid Content (TFC), and the DPPH assay were used to measure antioxidant activity. To induce PCOS in thirty female Wistar rats (180-200 g), letrozole (1 mg/kg) was used. For 15 days, BA leaf methanol extract (100 mg/kg body weight) and Clomiphene citrate (1 mg/kg body weight), a standard medication, were given. Microscopically, the ovaries underwent histopathological investigation. ELISA was used to assess luteinizing hormone (LH), follicle-stimulating hormone (FSH), and estradiol levels. One-way ANOVA was used to analyze the data, and P < 0.05 was set as the threshold for Dunnett's Multiple Comparison Test. LC50 value for the extract was 17.29±0.15 μg/mL. The DPPH radical scavenging activity of the ethyl acetate fraction was the highest. When PCOS animals were given BA, the ovarian stroma was normal and the granular cells were leutinized. The level of estradiol increased but the level of LH was not significantly different between the BA-treated group (0.23±0.03 mIU/mL) and the untreated PCOS group (0.23±0.01 mIU/mL). Basella alba leaf displayed ameliorative effects on polycystic ovary conditions in rats. This support the use of this plant in the management of PCOS.
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Joham AE, Norman RJ, Stener-Victorin E, Legro RS, Franks S, Moran LJ, Boyle J, Teede HJ. Polycystic ovary syndrome. Lancet Diabetes Endocrinol. 2022;10(9):668-680
Ugwu GO, Iyoke CA, Onah HE. Prevalence, presentation and management of polycystic ovary syndrome in Enugu, south east Nigeria. Niger J Med. 2013;22(4):313-6.
Rosenfield RL, Ehrmann DA. The pathogenesis of polycystic ovary syndrome (PCOS): the hypothesis of PCOS as functional ovarian hyperandrogenism revisited. Endocr Rev. 2016; 37:467-520.
Teede HJ, Misso ML, Costello MF, Dokras A, Laven J, Moran L, Piltonen T, Norman RJ. Recommendations from the international evidence-based guideline for the assessment and management of polycystic ovary syndrome. Hum Reprod. 2018; 33(9):1602-18.
Almeshari WK, Alsubaie AK, Alanazi RI, Almalki YA, Masud N, Mahmoud SH. Depressive and anxiety symptom assessment in adults with polycystic ovarian syndrome. Depress Res Treat. 2021;2021. Doi: 10.1155/2021/6652133
Ilhan M, Ali Z, Khan IA, Tastan H, Küpeli Akkol E. Isolation and identification of triterpenes from Anthemis austriacaJacq. through bioactivity-guided fractionation on polycystic ovary syndrome rat model. Arch. Gynecol. Obstet. 2020; 301:1103-11.
Gao L, Gu Y, Yin X. High serum tumor necrosis factor-alpha levels in women with polycystic ovary syndrome: a metaanalysis. PloS one. 2016; 11(10):e0164021.
Li T, Mo H, Chen W, Li L, Xiao Y, Zhang J, Li X, Lu Y. Role of the PI3K-Akt signaling pathway in the pathogenesis of polycystic ovary syndrome. Reprod Sci. 2017;24(5):646-55.
Escobar-Morreale HF: Polycystic ovary syndrome: definition, aetiology, diagnosis and treatment. Nat Rev Endocrinol. 2018; 14:270-84. Doi: 10.1038/nrendo.2018.24
Ndeingang EC, PB DD, Watcho P, Kamanyi A. Phyllanthus muellerianus (Euphorbiaceae) Restores Ovarian Functions in Letrozole-Induced Polycystic Ovarian Syndrome in Rats. Evid.-based Complement. Altern. Med. 2019; 2019:2965821-2965821
Rosenfield RL, Ehrmann DA. The pathogenesis of polycystic ovary syndrome (PCOS): the hypothesis of PCOS as functional ovarian hyperandrogenism revisited. Endocr. Rev. 2016; 37(5):467-520.
Louwers YV, Laven JS. Characteristics of polycystic ovary syndrome throughout life. Ther Adv Reprod Health. 2020; 14:1–9.
Younas A, Hussain L, Shabbir A, Asif M, Hussain M, Manzoor F. Effects of Fagonia indica on letrozole-induced polycystic ovarian syndrome (PCOS) in young adult female rats. Evid.-based Complement. Altern. Med. 2022;2022. Doi: 10.1155/2022/1397060
Matyanga CM, Dzingirai B. Clinical pharmacology of hormonal emergency contraceptive pills. International journal of reproductive medicine. 2018;2018. Doi: 10.1155/2018/2785839
Ogunlakin AD, Sonibare MA, Jabeen A, Shaheen F, Shah SF. Antiproliferative and ameliorative effects of Tetracera potatoria and its constituent. ADTM. 2021; 21:815-24.
Khanom B, Nahida U, Shapla SS, Akter MS, Farzana H, Zaman F, Islam MT. Phytochemical and pharmacological review on Basella alba L. Pharmacol. Online. 2019; 1:40-8.
Moutusi S, Prasannakumar MK, Kiranmayee P. Morphological and molecular characterization of culturable leaf endophytic fungi from Malabar Spinach: The first report. Studies in Fungi. 2019; 4(1):192-204.
Ogunlakin AD, Sonibare MA. Ethnobotanical survey of medicinal plants used as remedy for female infertility and menstrual disorder in southwestern Nigeria. Niger J Pharm Res. 2019; 15(2):205-17.
McLaughlin JL, Rogers LL, Anderson JE. The use of biological assays to evaluate botanicals. Drug Inf J, l998;32(l):5l3-524
Miliauskas G, Venskutonis PR, Van BTA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem, 2004; 85(2):231–237
Woisky R, Salatino A. Analysis of Propolis: some parameter and procedures for chemical quality control. J Apic Sci, l988.; 37(2):99–105
Bursal E, Gülçin I. Polyphenol content and in vitro antioxidant activities of lyophilized aqueous extract of kiwifruit (Actinidia deliciosa). Food Res Int, 2011; 44(5):l482–l489
Nair AB, Jacob S. A simple practice guide for dose conversion between animals and human. JBCP, 2016; 7(2):27-3l.
Ojo OA, Grant S, Amanze JC, Oni AI, Ojo AB, Elebiyo TC, Obafemi TO, Ayokunle DI, Ogunlakin AD. Annona muricata L. peel extract inhibits carbohydrate metabolizing enzymes and reduces pancreatic β-cells, inflammation, and apoptosis via upregulation of PI3K/AKT genes. Plos one. 2022; 17(10):e0276984.
Kafali H, Iriadam M, Ozardalı I, Demir N. Letrozole-induced polycystic ovaries in the rat: a new model for cystic ovarian disease. Arch Med Res, 2004; 35(2):103-l08.
Marcondes FK, Bianchi FJ, Tanno AP. Determination of the estrous cycle phases of rats: some helpful considerations. Braz J Biol, 2002; 62(4A):609-6l4.
Sudhakar P, Suganeswari M, Pushkalai SP, et al. Regulation of Estrous cycle using Combination of Gymnema sylvestre and Pergularia daemia in estradiol valerate induced PCOS rats. AsianJj. Res. Pharm. Sci. 2018; 8(1):4-8.
Avwioro, OG. Histochemistry and tissue pathology, principles and techniques. Nigeria: Claverianum, 2010.
Campbell DL, Lawton LA, Beattie KA, Codd GA. Comparative assessment of the specificity of the brine shrimp and microtox assays to hepatotoxic (microcystin‐LR‐containing) cyanobacteria. Environ. Toxicol. 1994; 9(1):71-7.
Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL. Brine Shrimp: A convenient general bioassay for active plant constituents. Planta Med, 1982; 45(5):3l-34.
Clarkson C, Maharaj VJ, Crouch NR, Grace OM, Pillay P, Matsabisa MG, Bhagwandin N, Smith PJ, Folb PI. In vitro antiplasmodial activity of medicinal plants native to or naturalized in South Africa. J Ethnopharmacol, 2004; 92(l):l77-l9l.
Van Wyk NE, Van Oualtshoorn B, Gericke N. Medicinal Plants of South Africa. Briza Publications, Pretoria S. Africa, l997.
Ogunlakin AD, Sonibare MA, Yeye OE, Gyebi GA, Ayokunle DI, Arigbede OE, Ibrahim IM, Odugbemi AI, Jabeen A, Shah SF, Shaheen F. Isolation and Characterization of Novel Hydroxyflavone from Kigelia africana (Lam.) Benth. Fruit Ethyl Acetate Fraction against CHO 1 and HeLa Cancer Cell Lines: In vitro and in silico studies. J Mol Struct. 2023; 2023:135180. Doi: 10.1016/j.molstruc.2023.135180
Omar AA, Hawazin HM, Asmita VP, Gerald B. Chemical composition and antioxidant activities of Jeddah corniche algae, Saudi Arabia, Saudi. J Biol Sci, 2009; 6(l):23–29.
Ogunlakin AD, Sonibare MA, Jabeen A, Shah SF, Shaheen F. Antioxidant and Anti-Proliferative Studies on Kigelia africana (Lam.) Benth. and its Constituents. Trop. J. Nat. Prod. Res. 2021; 5(3):570-5.
Amarowicz R, Pegg BR, Rahimi-Moghaddam P, Bar B, Weil JA. Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food Chem. 2003; 84(4): 55l-562.
Hussain T, Fatima I, Rafay M, Shabir S, Akram M, Bano S. Evaluation of antibacterial and antioxidant activity of leaves, fruit and bark of Kigelia africana. Pak J Bot. 20l6; 48(l): 277-283
Zhang C, Feng S, Wang Q, Wang P, Xu J, Chen T. Flavonoids and phenolic compounds from Smilax scobinicaulis. Chem Nat Compd. 20l4; 50(2):254-257
Corrie L, Gulati M, Singh SK, Kapoor B, Khursheed R, Awasthi A, Vishwas S, Chellappan DK, Gupta G, Jha NK, Anand K. Recent updates on animal models for understanding the etiopathogenesis of polycystic ovarian syndrome. Life Sci. 2021; 280:119753. Doi: 10.1016/j.lfs.2021.119753
Poojary PS, Nayak G, Panchanan G, Rao A, Kundapur SD, Kalthur SG, Mutalik S, Adiga SK, Zhao Y, Bakkum-Gamez J, Chang AY. Distinctions in PCOS induced by letrozole vs dehydroepiandrosterone with high-fat diet in mouse model. Endocrinol. 2022;163(9):bqac097. Doi: 10.1210/endocr/bqac097
Pachiappan S, Ramalingam K, Balasubramanian A. Combined effects of Gymnema sylvestre and Pergularia daemia on letrozole-induced polycystic ovarian syndrome in rats. Asian Pac. J. Reprod. 2021; 10(2):68-74.
Shende A, Joshi SS, Koli PG, Joshi S. Evaluation of the Effects of Caesalpinia crista on Letrozole-Induced Models of Polycystic Ovarian Syndrome. Cureus. 2023;15(1): e34215. Doi: 10.7759/cureus.34215
Tanruean K, Poolprasert P, Kumla J, et al. Bioactive compounds content and their biological properties of acetone extract of Cuscuta reflexa Roxb. grown on various host plants. Nat Prod Res. 2019; 33(4):544–547.
Al-mously SA, Gatea FK, Jawad E, Qasim B. The Effect of Capparis spinosa Extract on Induced PCOS Mice. Int. J.Pharm. Res. 2020;12(2). Doi: 10.31838/ijpr/2020.SP2.326
Saiyed A, Jahan N, Makbul SA, Ansari M, Bano H, Habib SH. Effect of combination of Withania somnifera Dunal and Tribulus terrestris Linn on letrozole induced polycystic ovarian syndrome in rats. Integr Med Res. 2016; 5(4):293-300.
Khosrowpour Z, Fahimi S, Jafari F, Tansaz M, Sahranavard S, Faizi M. Beneficial effects of Teucrium polium hydroalcoholic extract on letrozole-induced polycystic ovary syndrome (PCOS) in rat model. Korean J Obstet Gynecol. 2023; 66(2):107-117.
Ashkar F, Eftekhari MH, Tanideh N, Koohpeyma F, Mokhtari M, Irajie C, Iraji A. Effect of hydroalcoholic extract of Berberis integerrima and resveratrol on ovarian morphology and biochemical parameters in Letrozoleinduced polycystic ovary syndrome rat model: An experimental study. Int J Reprod BioMed. 2020; 18:637–650. Doi: 10.18502/ijrm.v13i8.7505