Pre-treatment with Hibiscus Sabdariffa Linn calyx Extract is Protective Against SubChronic Paracetamol Hepatotoxicity in Mice http://www.doi.org/10.26538/tjnpr/v7i4.18
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Many plant parts have been found to have efficient protective and ameliorative effects against drug induced liver injury. The present study aimed to investigate the prophylactic effects of Hibiscus sabdariffa Linn calyx aqueous extract (HSCE) on sub chronic paracetamol induced hepatotoxicity in mice. Twenty mice were randomly distributed into four groups of five mice each. The control and HSCE only group were given aqueous DMSO and HSCE (250 mg/kg b. wt.) respectively. Hepatotoxicity was induced in the mice by oral administration of paracetamol at a sub chronic dose (500 mg/kg b. wt.) in paracetamol only group. The pretreatment group were administered HSCE at zero time and paracetamol (500 mg/kg b. wt.) 8 h later. The blood and liver were collected and subjected to biochemical analysis after 8 weeks. The administration of paracetamol significantly (P ≤ 0.05) induced hepatotoxicity as evidenced by the increase in the level of liver marker enzymes and significant (P ≤ 0.05) decrease in the level of protein and albumin. Pretreatment with HSCE significantly (P ≤ 0.05) reduced serum levels of elevated liver enzyme markers. As regards to oxidative stress markers, pretreatment with HSCE significantly (P ≤ 0.05) decreased the level of malondialdehyde and increased the activities of superoxide dismutase and catalase. The hepatoprotective activity of HSCE was also confirmed by histopathological findings. These results suggest that pretreatment with HSCE has protective effect against paracetamol induced hepatotoxicity in mice.
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Corsini A, Bortolini M. Drug‐induced Liver injury: The role of drug metabolism and transport. J. Clin. Pharm. 2013; 53(5):463–474.
El Morsy EM, Kamel R. Protective effect of artichoke leaf extract against paracetamolinduced hepatotoxicity in rats. Pharm. Biol. 2015; 53(2):167-173.
Kuriyan R, Kumar DR, Rajedran R, Kurpad AV. An evaluation of the hypolipidemic effect of an extract of Hibiscus Sabdariffa leaves in hyperlipidemic Indians: A double blind, placebo controlled trial. BMC Complement Altern. Med. 2010; 10:27.
El-Saadany SS, Sitohy MZ, Labib SM, El-Massry RA. Biochemical dynamics and hypocholesterolemic action of Hibiscus sabdariffa (Karkade). Mol. Nutr. Food Res. 1991; 35(6):567-576.
Afolabi OC, Ogunsola FT, Coker AO. Susceptibility of cariogenic Streptococcus mutans to extracts of Garcinia kola, Hibiscus sabdariffa, and Solanum americanum. West Afr. J. Med.2008; 27(4): 230–233.
Peng CH, Chyau CC, Chan KC, Chan TH, Wang CJ, Huang CN. Hibiscus sabdariffa polyphenolic extract inhibits hyperglycemia, hyperlipidemia, and glycation-oxidative stress while improving insulin resistance. J. Agric. Food Chem. 2011; 59(18):9901–9909.
Obiefuna PCM, Owolabi OA, Adegunloye BJ, Obiefuna IP, Sofola OA. The petal extract of Hibiscus sabdariffa produces relaxation of isolated rat aorta. Pharm. Biol. 1994; 32(1):69-74.
Da-Costa-Rocha I, Bonnlaender B, Sievers H, Pischel I, Heinrich M. Hibiscus sabdariffa L.-a phytochemical and pharmacological review. Food Chem. 2014; 165: 424-443.
Dargan PI, Jones AL. Management of paracetamol poisoning. Trends in Pharmacol Sci.(TIPS) 2003; 24 (4):154–157.
Dafallah AA, Mustafa Z. Investigation of the anti-inflammatory activity of Acacia
nilotica and Hibicus sabdariffa. Am. J. Chin. Med. 1996; 24(3):263-9.
Dahiru D, Obi OJ, Umaru H. Effect of Hibiscus sabdariffa Linn calyx extract on carbon tetrachloride induced liver damage. Biokemistri 2003; 15:27-33.
Tabarak, M. Devendra, KP. and Nitu, D. Ameliorative potential of aqueous root extract of Withania somnifera against paracetamol induced liver damage in mice. Pharmacologia 2013; 4 (2): 89-94.
Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol. 1957; 28(1):56-63.
Tietz NW. Fundamentals of Clinical Chemistry. (2nd ed.). Philadelphia: WB Saunders Company; 1976. 897p.
Szasz G. A kinetic photometric method for serum gamma glutamyl transpeptidase. Clin Chem. 1969; 22:124(2)-136.
Trinder P. Enzymatic end point method. Annals of Clin Biochem. 1969; 6: 24.
Grant GH. Amino acids and proteins. In: Tietz NW. (Ed.). Fundamentals of Clinical Chemistry. Philadelphia USA: WB Saunders Company; 1987. 328-329p.
Tietz NW. Clinical Guide to Laboratory Tests. (3rd ed.). Philadelphia: WB Saunders Company; 1995. 518-519p.
Jendrassik L, Grof P. Simplified photometric methods for the determination of bilirubin. Biochemistry 1938; 297:81-89.
Misra HP, Fridovich I. The role of superoxide anion in the autooxidation of epinephrine and a simple assay of superoxide dismutase. J. Biol. Chem. 1972; 247(10):3170-3175.
Cohen G, Dembiee D, Marcus J. Measurement of catalase activity in tissue extracts. Anal. Bioch. 1970; 34(1):30-38.
Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol. 1978; 52: 302-310.
Tietz F. Enzymic method for quantitative determination of nanogram amount of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal. Bioch. 1969; 27(3):502-22.
Gu X, Manautou JE. Molecular mechanisms underlying chemical liver injury. Expert Rev. Mol. Med. 2012; 14:e4.
Ramachandran A, Jaeschke H. Mechanisms of acetaminophen hepatotoxicity and their translation to the human pathophysiology. J. Clin.Transl. Res. (JCTRES) 2017; 3(S1):157-169.
Lima IR, Silva IB, Lima RML, Silva TMS, Maia MBS, Leite SP. Hepatoprotective efficacy of methanolic extract of Indigofera suffruticosa (Mill) on paracetamol–induced liver damage in mice. Arq. Gastroenterol. 2019; 56(93):333-338.
Mahmoud HM, Abo-Youssef AM, Abo-Saif AA. Protective effect of Carvedilol on paracetamol-induced hepatotoxicity; role of modulation inflammation and lipid peroxidation. Int. J. Pharmacol. 2016; 13(1):33-43.
Soliman NA, El Dahmy SI, Shalaby AA, Mohammed KA, Taha NM. Ameliorative effects of Saccharum officinarum (sugarcane) peel extract against paracetamol-induced disorders in hematological indices and splenic changes in adult male Sprague Dawley rats. Trop. J. Nat.
Prod. Res. 2021; 5(11):1927-1933.
Chen Y, Dong H, Thompson DC, Shertzer HG, Nebert DW, Vasiliou V. Glutathione defense mechanism in liver injury: Insights from animal models. Food Chem. Toxicol. 2013; 60:38–44.
Ighodaro OM, Akinloye OA. First line defence antioxidants superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria J. Med. 2018; 54(4):287-293.
Liu LC, Wang CJ, Lee CC, Su SC, Chen HL, Hsu JD. Aqueous extract of Hibiscus sabdariffa L. decelerates acetaminophen-induced acute liver damage by reducing cell death and oxidative stress in mouse experimental models. J. Sci Food Agric. 2010; 90(2):329–337.
Olaleye MT, Rocha BT. Acetaminophen-induced liver damage in mice: Effects of some medicinal plants on the oxidative defense system. Exp. Toxicol. Pathol. 2008; 59(5):319–327.