Efficacy of Nitraria retusa L. Fruits Aqueous and Methanol Extracts as Antioxidant and Anti-inflammatory Activities on Carrageenan-Induced Paw Edema in Rats. http://www.doi.org/10.26538/tjnpr/v7i4.9

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Sahem Y Almasarwah
Sawsan A Oran
Rula M Darwish


The aim of this study was to assess the anti-oxidant and anti-inflammatory activities of aqueous and methanol extracts of Nitraria retusa against carrageenan induced paw edema in rats. The extracts were studied for antioxidant activity using Diphenyl-2-picryl-hydrazyl assay. Male Wister rats were divided into six groups including positive and negative controls. Group 1 (normal saline) as negative control, group 2 (150 mg/kg acetylsalicylic acid) as positive control, group 3 (100 mg/kg methanolic extract), group 4 (200 mg/kg methanolic extract), group 5 (100 mg/kg aqueous extract), group 6 (200 mg/kg aqueous extract). The results showed that aqueous and methanolic extract at doses of 100 and 200 mg/kg significantly decrease paw edema (P˂0.05). Also, the results revealed that methanol extract at 200 mg/kg is more effective in inhibiting paw edema (P˂0.005) than other doses and is equal to efficacy of acetylsalicylic acid. This finding indicated that N. retusa has an anti-inflammatory activity against carrageenan induced paw edema in rats. DPPH assay showed that methanol extract of N. retusa has high antioxidant activity (958.2±178.3 µg/mL) compared to aqueous extract (1249.3±233.5 µg/mL). In conclusion, N. retusa fruit extracts have an antioxidant activity and anti-inflammatory activity against carrageenan induced paw edema. 

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How to Cite
Almasarwah, S. Y., Oran, S. A., & Darwish, R. M. (2023). Efficacy of Nitraria retusa L. Fruits Aqueous and Methanol Extracts as Antioxidant and Anti-inflammatory Activities on Carrageenan-Induced Paw Edema in Rats.: http://www.doi.org/10.26538/tjnpr/v7i4.9. Tropical Journal of Natural Product Research (TJNPR), 7(4), 2725–2729. Retrieved from https://tjnpr.org/index.php/home/article/view/1871


Sun Q, Zhu J, Cao F, Chen F. Anti-inflammatory properties

of extracts from Chimonanthus nitens Oliv. leaf. PLoS

ONE. 2017; 12(7):1-19.

Furman D, Campisi J, Verdin E, Carrera-Bastos P, Targ S,

Franceschi C, Ferrucci L, Gilroy DW, Fasano A, Miller

GW, Miller AH, Mantovani A, Weyand CM, Barzilai N,

Goronzy JJ, Rando TA, Effros RB, Lucia A, Kleinstreuer N,

Slavich GM. Chronic Inflammation in the Etiology of

Disease Across the Life Span. Nat. Med. 2019;


Bindu S., Mazumder S., and Bandyopadhyay U. Nonsteroidal anti-inflammatory drugs (NSAIDs) and organ

damage: A current perspective. Biochem. Pharmacol. 2020;


Aminah NS, Tun KNW, Kristanti AN, Aung HT, Takaya Y,

Choudhary MI. Chemical constituents and their biological

activities from Taunggyi (Shan state) medicinal plants.

Heliyon. 2021; 7(2):e06173.

De La Cruz-Jiménez L, Hernández-Torres MA, MonroyGarcía IN, Rivas-Morales C, Verde-Star MJ, GonzalezVillasana V, Viveros-Valdez E. Biological Activities of

Seven Medicinal Plants Used in Chiapas, Mexico. Plants

(Basel). 2022; 11(14):1790-1802.

Oran S and Zeidan R. Antioxidant activity of leaf and fruit

extracts of Jordanian Rubus sanguineus Friv. (Rosaceae).

J. Med. Plant Res. 2014; 8(39): 1179–1190.

7Al-Eisawi D M H. Flora of Jordan Checklist, Revised;

Jafri S., El-Gadi A. Flora of Libya. Botany Department,

Faculty of Science, Al Fatteh University, Tripoli; 1977.

Boubaker J, ben Toumia I, Sassi A, Bzouich-Mokded I.,

Ghoul Mazgar S, Sioud F, Bedoui A, Safta Skhiri S,

Ghedira K, & Chekir-Ghedira L. Antitumoral Potency by

Immunomodulation of Chloroform Extract from Leaves of

Nitraria retusa, Tunisian Medicinal Plant, via its Major

Compounds β-sitosterol and Palmitic Acid in BALB/c Mice

Bearing Induced Tumor. Nutr Cancer. 2018; 70(4):650–

Chaâbane M, Koubaa M, Soudani N, Elwej A, Grati M,

Jamoussi K, Boudawara T, Chaabouni S E, Zeghal N.

Nitraria retusa fruit prevents penconazole-induced kidney

injury in adult rats through modulation of oxidative stress

and histopathological changes. Pharm. Biol. 2017; 55(1):


Chaâbane M, Soudani N, Benjeddou K, Turki M, Ayadi

Makni F, Boudawara T, Zeghal N, & Ellouze Ghorbel R.

The protective potential of Nitraria retusa on penconazoleinduced hepatic injury in adult rats. Toxicol Environ Chem.

; 97(9): 1253–1264.

Hussein SR, Kawashty SA, Tantawy

ME. Chemosystematic studies of Nitraria retusa and

selected taxa of Zygophyllaceae in Egypt. Plant Syst Evol.

; 277:251–264.

Majoumouo MS, Marius BT, Thureyah M, Donavon CH,

Fabrice FB, Chitra M. Antiproliferative potential of

methanolic and aqueous extracts and their methanolic

fractions derived from fruits of Bersama engleriana against

a panel of four cancer cell lines, Cogent Biol. 2020; 6(1):1-

Siddiqui N, Rauf A, Latif A, Mahmood Z.

Spectrophotometric determination of the total phenolic

content, spectral and fluorescence study of the herbal Unani

drug Gul-e-Zoofa (Nepeta bracteata Benth). J Taibah Univ

Med Sc. 2017; 12(4):360-363.

Chang CC, Yang H, Wen HM, Chern JC. Estimation of

Total Flavonoid Content in Propolis by Two

Complementary Colorimetric Methods. J Food Drug Anal.

; 10(3):178-182.

Paulpriya K, Lincy MP, Tresina PS, Mohan VR. In Vitro

Antioxidant Activity, Total Phenolic and Total Flavonoid

Contents of Aerial Part Extracts of Daphniphyllum

Neilgherrense (Wt.) Rosenth. J Biol Innov. 2015; 4(6):257-

Bolla SR, Mohammed Al-Subaie A, Yousuf Al-Jindan R,

Papayya Balakrishna J, Kanchi Ravi P, Veeraraghavan VP,

Arumugam Pillai A, Gollapalli SSR, Palpath Joseph J,

Surapaneni KM. In vitro wound healing potency of

methanolic leaf extract of Aristolochia saccata is possibly

mediated by its stimulatory effect on collagen-1 expression.

Heliyon. 2019; 5(5):e01648.

Amole OO, Akinyede AA, Obanyero DD. Toxicity Studies

of the Hydroethanolic Leaf Extract of Clerodendrum

polycephalum (Lamiaceae) in Rats. Ann. clin. Toxicol.

; 4(1):1-6.

Rajput MA, Zehra T, Ali F, Kumar G. Evaluation of

antiinflammatory activity of ethanol extract of Nelumbo

nucifera fruit. Turk J Pharm Sci. 2021; 18(1):56–60.

Hisamuddin N, Shaik Mossadeq WM, Sulaiman MR, Abas

F, Leong SW, Kamarudin N, Ong HM, Ahmad Azmi AF,

Ayumi RR, Talib M. Anti-Edematogenic and AntiGranuloma Activity of a Synthetic Curcuminoid Analog, 5-


hydroxyphenyl)penta-2,4-dien-1-one, in Mouse Models of

Inflammation. Molecules. 2019; 24(14):2614-2628.

Hossain MA, Shah MD, Gnanaraj C, Iqbal M. In vitro total

phenolics, flavonoids contents and antioxidant activity of

essential oil, various organic extracts from the leaves of

tropical medicinal plant Tetrastigma from Sabah. Asian Pac

J Trop Med. 2011; 4(9):717-721.

Abdulkhaleq LA, Assi MA, Abdullah R, Zamri-Saad M,

TaufiqYap YH, Hezmee M. The crucial roles of inflammatory mediator in inflammation: A review. Vet

World. 2018; 11(5):627-635.

Sadeghi H, Hajhashemi V, Minaiyan M, Movahedian A,

Talebi A. Further studies on anti-inflammatory activity of

maprotiline in carrageenan-induced paw edema in rat. Int.

Immunopharmacol.. 2013; 15(3):505-510.

Rjeibi I, Hentati F, Feriani A, Hfaiedh N, Delattre C,

Michaud P, Pierre G. Novel Antioxidant, Anti-α-Amylase,

Anti-Inflammatory and Antinociceptive Water-Soluble

Polysaccharides from the Aerial Part of Nitraria retusa.

Foods. 2019; 9(1): 28-45.

Alsarayreh A, Oran S, Shakhanbeh J. Evaluation of Antiinflammatory Activity of Methanol Extract of Rhus coriaria

L. in Diabetic Rats. Trop. J. Nat. Prod. Res. 2021; 5(8):


Al-Bzour J, Oran S, Mashallah K, Bustanji Y. Inhibitory

effects of methanolic extract of Salvia fruticosa Mill. on

pro-inflammatory cytokines production in RAW 264.7 in

vitro cellular model and in Balb/c mice in vivo animal

model. J. Med. Plant Res. 2011; 5(30):6662-6671.

Alsarayreh AZ, Oran S A, Shakhanbeh JM, Khleifat K M.,

Al Qaisi YT., Alfarrayeh I I, Alkaramseh AM. Efficacy of

methanolic extracts of some medicinal plants on wound

healing in diabetic rats. Heliyon. 2020; 8(8):e10071.

Al Qaisi YT., Khleifat KM., Alfarrayeh I I, Alsarayreh A Z.

In Vivo Therapeutic Effect of Some Medicinal Plants’

Methanolic Extracts on the Growth and Development of

Secondary Hydatid Cyst Infection. Acta Parasitol. 2020; 1-

Al Qaisi YT, Khleifat KM, Oran SA, Al Tarawneh AA,

Qaralleh H, Al-Qaisi TS, Farah HS. Ruta graveolens,

Peganum harmala, and Citrullus colocynthis methanolic

extracts have in vitro protoscolocidal effects and act against

bacteria isolated from echinococcal hydatid cyst fluid. Arch.

Microbiol. 2022; 204(4):228-231.

Falodun A, Okunrobo LO and Uzoamaka

N. Phytochemical screening and anti-inflammatory

evaluation of methanolic and aqueous extracts of Euphorbia

heterophylla Linn (Euphorbiaceae). Afri J

Biotechnol. 2006; 5, 529 - 531.

Falodun A, Okunrobo LO and Agbo LO. Evaluation of the

anti-edematogenic activity of Leea guineensis leaf. Afri J

Biotechnol 2007; 6 (9):1151–1153.