Efficacy of Nitraria retusa L. Fruits Aqueous and Methanol Extracts as Antioxidant and Anti-inflammatory Activities on Carrageenan-Induced Paw Edema in Rats.



  • Sahem Y Almasarwah Department of Biological Sciences, Faculty of Sciences, University of Jordan, Amman, Jordan.
  • Sawsan A Oran Department of Biological Sciences, Faculty of Sciences, University of Jordan, Amman, Jordan.
  • Rula M Darwish Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Jordan, Amman, Jordan


Inflammation, Paw edema, Diphenyl-2-picryl-hydrazyl, Nitraria retusa, a


The aim of this study was to assess the anti-oxidant and anti-inflammatory activities of aqueous and methanol extracts of Nitraria retusa against carrageenan induced paw edema in rats. The extracts were studied for antioxidant activity using Diphenyl-2-picryl-hydrazyl assay. Male Wister rats were divided into six groups including positive and negative controls. Group 1 (normal saline) as negative control, group 2 (150 mg/kg acetylsalicylic acid) as positive control, group 3 (100 mg/kg methanolic extract), group 4 (200 mg/kg methanolic extract), group 5 (100 mg/kg aqueous extract), group 6 (200 mg/kg aqueous extract). The results showed that aqueous and methanolic extract at doses of 100 and 200 mg/kg significantly decrease paw edema (P˂0.05). Also, the results revealed that methanol extract at 200 mg/kg is more effective in inhibiting paw edema (P˂0.005) than other doses and is equal to efficacy of acetylsalicylic acid. This finding indicated that N. retusa has an anti-inflammatory activity against carrageenan induced paw edema in rats. DPPH assay showed that methanol extract of N. retusa has high antioxidant activity (958.2±178.3 µg/mL) compared to aqueous extract (1249.3±233.5 µg/mL). In conclusion, N. retusa fruit extracts have an antioxidant activity and anti-inflammatory activity against carrageenan induced paw edema. 


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How to Cite

Almasarwah, S. Y., Oran, S. A., & Darwish, R. M. (2023). Efficacy of Nitraria retusa L. Fruits Aqueous and Methanol Extracts as Antioxidant and Anti-inflammatory Activities on Carrageenan-Induced Paw Edema in Rats.: http://www.doi.org/10.26538/tjnpr/v7i4.9. Tropical Journal of Natural Product Research (TJNPR), 7(4), 2725–2729. Retrieved from https://tjnpr.org/index.php/home/article/view/1871