Effect of Curcumin (Standard and Supplement) with Zinc on Reproductive Hormones in Polycystic Ovary Syndrome (PCOS) Rats
http://www.doi.org/10.26538/tjnpr/v7i4.5
Keywords:
Zinc, Polycystic ovary syndrome, Metformin, Letrozole, Luteinizing hormone, Follicle-stimulating hormone, CurcuminAbstract
Polycystic ovary syndrome (PCOS) is a complex and heterogeneous disorder that affects a large percentage of women worldwide. The pathophysiology is not fully explained. In recent years, complementary and alternative medicine has emerged as a viable treatment option. This study examined how curcumin and zinc affected on reproductive hormones in rats with polycystic ovary syndrome (PCOS). It was induced by oral administration of letrozole (1mg/kg/day) for 21 days. PCOS groups were treated over 14 days with curcumin standard 200 mg/kg, curcumin supplement 200 mg/kg, and zinc 30 mg /kg (separately or in combination with two forms of curcumin), and metformin 50 mg/kg as standard treatment. The blood biochemistry of these groups was compared to those of the groups of healthy, PCOS, and those who received metformin. The immunoassay ELISA technique was employed for measuring the concentration of reproductive hormones. The results manifested that the administration of letrozole resulted in a significant elevation (p≤0.05) of luteinizing hormone, follicle-stimulating hormone, testosterone, and prolactin associated with a significant decrease (p≤0.05) of estrogen and progesterone levels in the PCOS-designed model. The results of effect of curcumin supplement alone and with zinc showed a significant improvement in all parameters by reducing the luteinizing hormone, follicle-stimulating hormone, testosterone, and prolactin. Estrogen and progesterone elevated significantly for all groups, while the elevation was highly significant in both curcumin supplement alone and curcumin supplement with zinc. The treatment with curcumin supplement and their combination with zinc showed
promising results in restoring reproductive hormones to the normal level.
References
Lentscher JA, Decherney AH. Clinical Presentation and Diagnosis of Polycystic Ovarian Syndrome. Clin Obstet Gynecol. 2021; 64(1):3-11.
Aziz KMA. Insulin Resistance, Obesity and Polycystic Ovarian Syndrome in Diabetic Patients. Diabetes Obes Int J. 2018; 3(1):000173.
Fazel F, Torshizi, Chamani M, Khodaei HR, Sadeghi AA, Hejazi SH, Majidzadeh R Heravi. Therapeutic effects of organic zinc on reproductive hormones, insulin resistance, and mTOR expression, as a novel component, in a rat model of polycystic ovary syndrome. Iran J Basic Med Sci. 2020; 23(1):36-45.
Shaaban Z, Khoradmehr A, Jafarzadeh MR Shirazi, Tamadon A. Pathophysiological mechanisms of gonadotropins and steroid hormones-related genes in etiology of polycystic ovary syndrome. Iran J Basic Med Sci. 2019; 2(1):3-16.
Azziz R. Polycystic Ovary Syndrome. Obstet Gynecol. 2018; 132(2):321-336.
Rocha AL, Oliveira FR, Azevedo RC, Silva VA, Peres TM, Candido AL, Gomes KB, Reis FM. Recent advances in the understanding and management of polycystic ovary syndrome. F1000Res. 2019 Apr ; 8: 565-575 .
Ihsan L, Tehreem A, Rasool S. Significance of TNF-alpha and insulin resistance in women with the polycystic ovarian syndrome. Pakistan J Med Health Sci. 2018; 12(2):459-463.
Al-Fartosy A, Awad N, Mohammed A H. Intelectin-1 and Endocrinological Parameters in Women with Polycystic Ovary Syndrome: Effect of Insulin Resistance. Ewha Med J. 2020; 43(1):1-11.
Mvondo MA, Mzemdem FI, Awounfack CF, Njamen D. The leaf aqueous extract of Myrianthus arboreus P. Beauv.(Cecropiaceae) improved letrozole-induced polycystic ovarian syndrome associated conditions and infertility in female Wistar rats. BMC Complement Med Ther. 2020;
(1):275 -287.
Hasan K, Mehmet I, Ilyas O, Nurettin D. Letrozole-induced polycystic ovaries disease. Arch Med Res.2004; 35(2):103-108.
Mohsen S. Al-Mozie’l. Role of metformin, spironolactone treatment, and their combination in estradiol valerate induced polycystic ovarian female rats. The College of Veterinary Medicine - University of Basrah. Ph.D. thesis. 2014.
Jakubczyk K, Drużga A, Katarzyna J, Skonieczna K. Antioxidant Potential of Curcumin-A Meta Analysis of Randomized Clinical Trials. Antioxidants (Basel). 2020; 9(11):1092 -1104 .
Her C, Venier MC, Roger E. Improvement of Curcumin Bioavailability for Medical Applications. Med Aromat Plants (Los Angeles). 2018; 7(6):326 -340.
Ng X, Soh AYS, Loke W, Venkatanarayanan N, Lim DY, Yeo WS. A Meta-Analysis of the Clinical Use of Curcumin for Irritable Bowel Syndrome (IBS). J. Clin. Med. 2018; 7(10):298.
Fadus MC, Lau C, Bikhchandani J, Lynch HT. Curcumin: An age-old anti-inflammatory and anti-neoplastic agent. J Tradit Complement Med. 2016; 7(3):339-346.
Guerrero F, Simental LE, Martínez G, Sánchez MA, Gamboa CI. Hypoglycemic and antioxidant effects of five commercial turmerics (Curcuma longa) supplements. J Food Biochem. 2020; 44(9): e13389. doi: 10.1111/jfbc.13389
Fan C, Song Q, Wang P, Li Y, Yang M, Yu SY. Neuroprotective Effects of Curcumin on IL-1beta-Induced Neuronal Apoptosis and Depression-Like Behaviors Caused by Chronic Stress in Rats. Front Cell Neurosci. 2018; 7(12):516 -532.
Mohmmed AH, Awad NA, AL-Fartosy AJ. Study of Trace Elements Selenium, Copper, Zinc and Manganese Level in Polycystic Ovary Syndrome (PCOS). International Journal for Research in Applied Sciences and Biotechnology. 2019; 6(8):16-22.
Musadak A, Zearah SA. Effects of Serotonin, Melatonin, and Zinc Levels on Prolactin Hormone in Hyperprolactinemic Patients in Basrah Governorate, Iraq. Trop J Nat Prod Res. 2022; 6(5): 740-744.
Pokorska K, Brodowska A, Szczuko M. The Content of Minerals in the PCOS Group and the Correlation with the Parameters of Metabolism. Nutrients. 2021; 13(7):2214.
Jurenka JS. Anti-inflammatory properties of curcumin, a major constituent of Curcuma longa: a review of preclinical and clinical research. Altern Med Rev. 2009; 14(2):141153.
Tabanelli R, Brogi S, Calderone V. Improving Curcumin Bioavailability: Current Strategies and Future Perspectives. Pharmaceutics. 2021; 13(10):1715 -1751.
Shoba G, Joy D, Joseph T, Majeed M. Rajendran, R, Srinivas, P.S. Influence of piperine on the pharmacokinetics of curcumin in animals and human volunteers. Planta Med. 1998; 64(4):353-356.
Reddy PS, Begum N, Mutha S, Bakshi V. Beneficial effect of curcumin in letrozole induced polycystic ovary syndrome. Asian Pac J Reprod. 2016; 5:116–122.25. Simoni M, Gromoll J, Nieschlag E. The follicle-stimulating hormone receptor: Biochemistry, molecular biology, physiology, and pathophysiology. Endocrinology Reviews. 1997; 18(1):739–773
Uotila M, Ruoslahti E, Engvall E. Two-site sandwich enzyme immunoassay with monoclonal antibodies to human alpha-fetoprotein. Journal of Immunological Methods. 1981; 42(1), 11–15.
Bergquist C, Nillius SJ, Wide L. Human gonadotropin therapy. I. Serum estradiol and progesterone patterns during conceptual cycles. Fertility and Sterility. 1983; 39(6): 761–765.
Chen A, Bookstein JJ, Meldrum DR. Diagnosis of a testosterone-secreting adrenal adenoma by selective venous catheterization. Fertility and Sterility. 1991; 55(6):1202–1203.
Mohammadi S, Bardei LK, Hojati V, Ghorbani A, Nabiuni M. Anti-inflammatory effects of curcumin on insulin resistance index, levels of interleukin-6, C-reactive protein, and liver histology in polycystic ovary syndrome-induced rats. Cell. J. 2017; 19(3):425-433.
Amoura M, Abou-El-Naga LZ, Habbak NE, El Bakary KA, El-Sharawy A. Potential effects of Mentha piperita (peppermint) on Letrozole - induced polycystic ovarian syndrome in female albino rat. Intern J of Advance Res. 2015; 3(10):211-226
Jena D, Choudhury A, Mangaraj S, Singh M, Mohanty B, Baliarsinha A. Study of visceral and subcutaneous abdominal fat thickness and its correlation with cardiometabolic risk factors and hormonal parameters in polycystic ovary syndrome. Indian J Endocrinol Metab. 2018; 22(3):321-327.
Milutinović DV, Nikolić M, Veličković N, Djordjevic A, Bursać B, Nestorov J, Teofilović A, Antić IB, Macut JB, Zidane AS, Matić G, Macut D. Enhanced Inflammation without Impairment of Insulin Signaling in the Visceral Adipose Tissue of 5α-Dihydrotestosterone-Induced Animal
Model of Polycystic Ovary Syndrome. Exp Clin Endocrinol Diabetes. 2017; 125(8):522-529.
Jassim A, Fouad H. Ameliorative Effect of Fenugreek on sex hormones in Polycystic Ovary Syndrome Female Rats Induced by Letrozole . K J V S. 2017; 8:24-32.
Haslan MA, Samsulrizal N, Hashim N, Zin NSNM, Shirazi FH, Goh YM. Ficus deltoidea ameliorates biochemical, hormonal, and histomorphometric changes in letrozoleinduced polycystic ovarian syndrome rats. BMC Complement Med Ther. 2021; 21(1):291 -303.
Chasapis CT, Ntoupa SA, Spiliopoulou CA, Stefanidou ME. Recent aspects of the effects of zinc on human health. Arch Toxicol. 2020; 94:1443–1460.
Shukrya H, Alwan, Muna H. Al-Saeed. .Biosynthesized Silver Nanoparticles (using CinnamomumZeylanicum Bark Extract) Improve the Fertility Status of Rats with Polycystic Ovarian Syndrome .Biocatal Agric Biotechno. 2021; 38: 102217.
Lord JM, Flight IH, Norman RJ. Metformin in polycystic ovary syndrome: systematic review and meta-analysis. BMJ. 2003; 327(7421):951-953.
Sam S, Ehrmann DA. Metformin therapy for the reproductive and metabolic consequences of polycystic ovary syndrome. Diabetologia. 2017; 60:1656- 1661.
Suhaimi NA, Nooraain H, Nurdiana S. Effects of Ficus deltoidea Ethanolic leaves extract on female reproductive organs among letrozole-induced polycystic ovarian syndrome rats. J Sci Res Dev. 2016; 3(4):8–14.
Rebecca L. Edwards, Paula B. Luis, Paolo V. Varuzza, Akil I. Joseph, Sai H. Presley, Rupesh C, Claus S. The antiinflammatory activity of curcumin is mediated by its oxidative metabolites. Journal List J Biol Chem. 2017; 292(52):21243–21252.
Dai W, Wang H, Fang J, Zhu Y, Zhou J, Wang X. Curcumin provides neuroprotection in model of traumatic brain injury via the Nrf2-ARE signaling pathway. Brain Res. Bull. 2018; 140:65–71.
Xu Y, Meng X, Li S, Gan Y, Li Y, Li B. Bioactivity, health benefits, and related molecular mechanisms of curcumin: Current progress, challenges, and perspectives. Nutrients. 2018; 10(10):1553. doi: 10.3390/nu10101553.
Yan Z, Dai Y, Fu H, Zheng Y, Bao D, Yin Y, Chen Q, Nie X, Hao Q, Hou D. Curcumin exerts a protective effect against premature ovarian failure in mice. J. Mol. Endocrinol. 2018; 60(3):261–271.
Karandish M, Mozaffari-Khosravi H, Mohammadi SM, Azhdari M, Cheraghian B. Evaluation of the effect of curcumin and zinc co-supplementation on glycemic measurements, lipid profiles, and inflammatory and antioxidant biomarkers in overweight or obese prediabetic
patients: a study protocol for a randomized double-blind placebo-controlled phase 2 clinical trial. Trials. 2020; 21(1):991.
Zhang Y, Weng Y, Wang D, Wang R, Wang L, Zhou J, Shen S, Wang H, Wang Y. Curcumin in Combination with Aerobic Exercise Improves Follicular Dysfunction via Inhibition of the Hyperandrogen-Induced IRE1α/XBP1 Endoplasmic Reticulum Stress Pathway in PCOS-Like Rats. Oxid Med Cell Longev. 2021;2021:7382900.
Bachmeier BE, Mirisola V, Romeo F, Generoso L, Esposito A, Dell'eva R, Blengio F, Killian PH, Albini A, Pfeffer U. Reference profile correlation reveals estrogen-like transcriptional activity of Curcumin. Cell Physiol Biochem. 2010; 26(3):471-82. 47. Haixin L, Shuang H, Taiyi W, Barnabas Orang-OO, Qing L, Zhongqun Z, Lanlan P, Xin C, Honghua W, Guanwei F, Peng Z, Yuxin F, Yun SS, Xuimei G, Richard HK, Yan Z. Selected
Phytoestrogens Distinguish Roles of ERα Transactivation and Ligand Binding for Anti-Inflammatory Activity, Endocrinology. 2018; 159(9):3351–3364.
Fatemi SM, Khanbabaei R, Hayati N, Parivar K, Yaghmaei P. Curcumin-loaded super-paramagneticiron oxide nanoparticle effects on apoptotic factors expression and histological changes in a prepubertal mouse model ofpolycystic ovarysyndrome-induced by dehydroepiandrosterone - A molecular and stereological study. Life Sci. 2020; 249:117515.
Baptiste C, Battista MC, Trottier A, Baillargeon JP. Insulin and hyperandrogenism in women with polycystic ovary syndrome. J Steroid Biochem Mol Biol. 2010; 122(1-3):42–52.
Guler O, Turp A, Erdem A, Erdem M, Onan MA, Taskiran C. Zinc and homocysteine levels in polycystic ovarian syndrome patients with insulin resistance. Biol Trace Elem Res. 2014; 158(3):297–304.
Elobeid T, Awad MO, Ganji V, Moawad J. The Impact of Mineral Supplementation on Polycystic Ovarian Syndrome. Metabolites. 2022; 12(4):338.
Beletate V, El Dib R, Atallah A. Zinc supplementation for the prevention of type 2 diabetes mellitus. Cochrane Database of Systematic Reviews. 2007; 1:CD005525.
Chakraborty P, Ghosh S, Goswami S. Altered Trace Mineral Milieu Might Play an Aetiological Role in the Pathogenesis of Polycystic Ovary Syndrome. Biol Trace Elem Res. 2013; 152(1):9-15.
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Tropical Journal of Natural Product Research (TJNPR)
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
