In vivo Anti-Inflammatory Activity of Coleus atropurpureus Leaves Extract and Fractions doi.org/10.26538/tjnpr/v6i1.8

Main Article Content

Ipang Djunarko
Nanang Fakhrudin
Arief Nurrochmad
Subagus Wahyuono

Abstract

Inflammation is a normal body response to injury. However, inflammation also contributes to


the progression of various diseases including cancer, atherosclerosis, asthma, obesity, and


rheumatoid arthritis. Thus, the discovery of antiinflammatory agents remains chalenging.


Medicinal plant is a potential source of drug discovery, including for antiinflammatory agents.


One of the medicinal plants traditionally used for treating inflammatory diseases is Coleus


atropurpureus. This study evaluated the in vivo anti-inflammatory effect of Coleus


atropurpureus leaves extract and its fractions. Wistar rats were divided into 5 groups: negative


control (solvent treatment), positive control (Diclofenac 9 mg/kg BW), ethanol extract (EE), n


hexane fraction (HF), and ethanol-water fraction (EWF). The extract and fractions were given at


the same dose (45 mg/kg BW) 6 h prior to the induction of inflammation using carrageenan.


Thin layer chromatography (TLC) analysis was done to identify the chemical components of the


extract and fractions. The ethanol extract (EE), n-hexane fraction (HF), and ethanol-water


fraction (EWF) reduced paw oedema thickness by 23.66, 19.01, and 20.80%, respectively,


compared to the negative control. TLC analysis revealed the difference in the phytochemical


content of the fractions. The HF mainly contained terpenoids, whereas the EFW contained


flavonoids. This study demonstrated that EE, HF, and EWF of C. atropurpureus leaves have


antiinflammatory activity in carrageenan-induced paw edema in rats.


 

Article Details

How to Cite
Djunarko, I., Fakhrudin, N., Nurrochmad, A., & Wahyuono, S. (2022). In vivo Anti-Inflammatory Activity of Coleus atropurpureus Leaves Extract and Fractions: doi.org/10.26538/tjnpr/v6i1.8. Tropical Journal of Natural Product Research (TJNPR), 6(1), 40–43. Retrieved from https://tjnpr.org/index.php/home/article/view/186
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Author Biography

Ipang Djunarko, Postgraduate Program of Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia

Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Sanata Dharma University Yogyakarta Campus III, 55282, Indonesia

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