In vivo Anti-Inflammatory Activity of Coleus atropurpureus Leaves Extract and Fractions


  • Ipang Djunarko Postgraduate Program of Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
  • Nanang Fakhrudin Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
  • Arief Nurrochmad Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
  • Subagus Wahyuono Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia


Coleus, Paw oedema, Inflammation, Thin layer chromatography


Inflammation is a normal body response to injury. However, inflammation also contributes to

the progression of various diseases including cancer, atherosclerosis, asthma, obesity, and

rheumatoid arthritis. Thus, the discovery of antiinflammatory agents remains chalenging.

Medicinal plant is a potential source of drug discovery, including for antiinflammatory agents.

One of the medicinal plants traditionally used for treating inflammatory diseases is Coleus

atropurpureus. This study evaluated the in vivo anti-inflammatory effect of Coleus

atropurpureus leaves extract and its fractions. Wistar rats were divided into 5 groups: negative

control (solvent treatment), positive control (Diclofenac 9 mg/kg BW), ethanol extract (EE), n

hexane fraction (HF), and ethanol-water fraction (EWF). The extract and fractions were given at

the same dose (45 mg/kg BW) 6 h prior to the induction of inflammation using carrageenan.

Thin layer chromatography (TLC) analysis was done to identify the chemical components of the

extract and fractions. The ethanol extract (EE), n-hexane fraction (HF), and ethanol-water

fraction (EWF) reduced paw oedema thickness by 23.66, 19.01, and 20.80%, respectively,

compared to the negative control. TLC analysis revealed the difference in the phytochemical

content of the fractions. The HF mainly contained terpenoids, whereas the EFW contained

flavonoids. This study demonstrated that EE, HF, and EWF of C. atropurpureus leaves have

antiinflammatory activity in carrageenan-induced paw edema in rats.


Author Biography

Ipang Djunarko, Postgraduate Program of Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia

Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Sanata Dharma University Yogyakarta Campus III, 55282, Indonesia


Furman D, Campisi J, Verdin E, Carrera-Bastos P, Targ S, Franceschi, Ferrucci L, Gilroy DW, Fasano A, Miller GW, Miller AH. Chronic inflammation in the etiology of disease across the life span. Nat Med. 2019; 25:1822-1832.

Sharma V, Tiwari RK, Shukla SS, Pandey RK. Current and future molecular mechanism in Inflammation and Arthritis. J Pharmacopuncture. 2020; 23(2):54-61.

Cotter J and Wooltorton E. New restrictions on celecoxib (Celebrex) use and the withdrawal of valdecoxib (Bextra). CMAJ. 2005; 172:1299-1299.

Yasir M, Goyal A, Bansal P, Sonthalia S. Corticosteroid Adverse Effects. 2021 Jul 8. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2022 Jan–. PMID: 30285357.

Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol. 2014; 4:177.

Azab A, Nassar A, Azab AN. Anti-inflammatory activity of natural products. Molecules. 2016; 21(10):1321.

Fürst R and Zündorf I. Plant-derived anti-inflammatory compounds: Hopes and disappointments regarding the translation of preclinical knowledge into clinical progress. Mediators Inflamm. 2014; 2014:146832.

Lachkar N, Al-Sobarry M, El Hajaji H, Lamkinsi T, Lachkar M, Cherrah Y, Alaoui K. Anti-inflammatory and antioxidant effect of Ceratonia siliqua L. methanol barks extract. J Chem Pharm Res. 2016; 8:202-210.

Raj SS. Uses of medicinal plants for anti-inflammatory activity-a review. Eur J Mol Clin Med. 2020; 7(1):1839-1843.

Amitjitraresmu A. Anti-inflammatory effect of diverse extracts of Coleus atropurpureus, l. benth leaves and its phytochemical screening. BPPK DepKes RI, Jakarta, Indonesia: 1995; 131p.

Sagala N. Anti-inflammatory effect of Coleus atropurpureus L. Benth leaves infuse with dose 140 mg/KgBW combined with Clitoria ternatea L. flower dose 328; 655; 1310 mg/kgBW at paw oedema in female mice induced by carrageenan using callipers. Final Project Report. 2013;Universitas Sanata Dharma, Indonesia.

Fakhrudin N, Pertiwi KK, Takubessi MI, Susiani EF, Nurrochmad A, Widyarini S, Sudarmanto A, Nugroho AA, Wahyuono S. A geranylated chalcone with antiplatelet activity from the leaves of breadfruit (Artocarpus altilis). Pharmacia. 2020; 67:173.

Fakhrudin N, Putri PS, Sutomo S, Wahyuono S. Antiinflamatory activity of methanolic extract of Mangifera casturi in thioglycollate-induced leukocyte migration on mice. MOT. 2013; 18:151-156.

Sinurat JP, Krisdianilo V, Karo RMB, Berutu R. Analysis of total terpenoids from Maniltoa grandiflora (A. Gray) Scheff leaves using TLC and HPLC methods. Stannum: Jurnal Sains Dan Terapan Kimia. 2020; 2:40-44.

Nansy E, Pramono S, Nugroho AE. Total flavonoid content and in vivo hypotensive effect of chloroform insoluble fraction of Centella asiatica leaf extract. Int Food Res J. 2015; 22(5):2119-2125.

Abdul–Azeez MR and Al–Fartosy AJ. Preparation of some gels polymeric networks sustained drug Prednisolone antiinflammatory and study as slow release (in vivo) and (in vitro). J Missan Res. 2016; 12:15-29.

Tjandrawinata RR, Djunarko I, Fenty HP, Hendra P. Antiinflammation effects of bioactive fraction DLBS0533 containing Phaleria macrocarpa and Nigella sativa on animal model. Int J Pharm Pharm Sci. 2015; 7:408-11.

Blomme EA and Will Y. Toxicology strategies for drug discovery: present and future. Chem Res Toxicol. 2016; 29:473-504.

Ma Y, Li Y, Li X, Wu Y. Anti-inflammatory effects of 4-methylcyclopentadecanone on oedema models in mice. Int J Mol Sci. 2013; 14:23980-23992.

Posadas I, Bucci M, Roviezzo F, Rossi A, Parente L, Sautebin L, Cirino G. Carrageenan‐induced mouse paw ooedema is biphasic, age‐weight dependent and displays differential nitric oxide cyclooxygenase‐2 expression. Br J Pharmacol. 2004; 142:331-338.

Harris GK, Qian Y, Leonard SS, Sbarra DC, Shi X. Luteolin and chrysin differentially inhibit cyclooxygenase-2 expression and scavenge reactive oxygen species but similarly inhibit prostaglandin-E2 formation in RAW 264.7 cells. J Nutr. 2006; 136:1517-1521.

Fakhrudin N, Khairunnisa SY, Azzahra A, Ajiningtyas RJ. Study of radical scavenger activity, total phenol and flavonoid contents of Artocarpus altilis leaves extracts. Int J Pharm Clin Res. 2016; 8:352-356.

Iqbal H and Singh D. Antioxidants: A Brief Review. South Asian Res J Med Sci. 2019; 1:36-39.

Heldin CH, Lu B, Evans R, Gutkind JS. Signals and receptors. Cold Spring Harb Perspect Biol. 2016; 8(4):a005900.

Raker VK, Becker C, Steinbrink K. The cAMP pathway as therapeutic target in autoimmune and inflammatory diseases. Front Immunol. 2016; 7:123.

Asia Maya. Iler (Coleus atropurpureus L. Benth). [online]. [cited 2021 Sept 5]. Available from: http://www.asimaya.comjamuisiilr_coleusatropurpureus.htm .

Lenny S, Barus T, Marpaung L, Nasution MP. Structure elucidation of flavonoid compound from the leaves of Coleus atropurpureus benth using 1D-and 2D-NMR techniques. MJAS. 2013; 17:255-261.

Verawati V, Aria M, Dira D, Maisa S, Maharani A. Chemical characterization and anti-inflammatory activity of Piladang Leaf (Coleus Atropurpureus) extract. J Chem Pharm Sci. 2016; 6:2496-2499.




How to Cite

Djunarko, I., Fakhrudin, N., Nurrochmad, A., & Wahyuono, S. (2022). In vivo Anti-Inflammatory Activity of Coleus atropurpureus Leaves Extract and Fractions: Tropical Journal of Natural Product Research (TJNPR), 6(1), 40–43. Retrieved from

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