ERRATUM: Evaluation of the Anti-inflammatory, Antioxidant, and Protease Inhibitory Activity of the Crude Methanol Extract of Portulaca oleracea. http://.www.doi.org/10.26538/tjnpr/v7i2.15
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Abstract
Protease inhibitors have considerable applications in biomedicine, biotechnology, and therapy.
The aim of the study was to evaluate the methanol crude extracts of aerial portions of Portulaca
oleracea (P. oleracea) for anti-inflammatory, antioxidant, and protease inhibitor activities, and to
evaluate their inhibitory action against various therapeutically significant proteases. A modified
colorimetric assay was used to analyze the protease inhibitors on trypsin and other therapeutic
enzymes. P. oleracea crude leaf extract exhibited 79.92% antioxidant and 75% anti-inflammatory
activity. Analysis of the effects of crude P. oleracea extract on the values of enzyme kinetics (Km,
Vmax) was determined by Lineweaver-Burk reciprocal plot. It was found that the extract exhibited
a potent inhibitory effect on the Vmax (µmole/min) of the chymotrypsin 26.0, trypsin 18.0, papain
8.33, and elastase 10.0, when compared to control 41.0, 73.0, 11.11, and 20.3, respectively. While
no effect on Km except for elastase which was decreased from 0.026 mM for the control to 0.01
mM. It was found that this effect showed a powerful inhibition in a different way. There is a mixed
non-competitive inhibition on elastase as well as pure non-competitive inhibition on
chymotrypsin, trypsin, and papain. The crude extract contained 53.3%, 55.7%, 14.9%, and 26.9%,
respectively, of protease inhibitors for the therapeutic protease enzymes trypsin, papain,
chymotrypsin, and elastase. Therefore, trypsin, papain, chymotrypsin, and elastase have been
inhibited by the crude extract of P. oleracea. Characterization of the crude extract indicated potent
anti-inflammatory and antioxidant effects.
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