Effects of Pegagan Embun (Hydrocotyle sibthorpioides Lam) Extract on Renal Function in Male Wistar Rats as Assessed by Creatinine Clearance http://www.doi.org/10.26538/tjnpr/v7i3.13

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Yufri Aldi
Afriwardi Afriwardi
Elsa Badriyya
Wanda S. Azukhruf
Aditya A. Alianta

Abstract

In Indonesia, pegagan embun (Hydrocotyle sibthorpioides Lam), a low-growing plant native to southeastern Asia, has been used as a traditional medicine to treat numerous ailments. However, additional research on the safety of this plant is needed, particularly before it can be promoted as a potential immune system-boosting drug. The present study aimed to evaluate the sub-acute toxicity effects of ethanol extract of Hydrocotyle sibthorpioides (HS) on renal function. Thirtysix male Wistar rats divided into four groups were used for this study. The control group was given NaCMC (0.5%), and the three treatment groups received an oral dose of the extract of HS at doses of 7, 35, and 150 mg/kg BW, respectively, for 21 days. The parameters measured include: 24-hour urine volume, serum creatinine, urine creatinine, creatinine clearance, and the percentage of renal function on the 7th, 14th, and 21st days were determined. The results showed that doses of HS extract at 7, 35, and 150 mg/kg BW significantly affected creatinine clearance (p<0.05). In contrast, the duration of administration has no significant effect on creatinine clearance (p>0.05). Renal function increased above the standard value (>100%) at 7 and 35 mg/kg BW doses. Thus, these concentrations could potentially cause impairments in renal function. The study concludes that various doses of the ethanol extract of Hydrocotyle sibthorpioides affected renal creatinine clearance in Wister rats. In contrast, the duration of administration of Hydrocotyle sibthorpioides extract did not affect creatinine clearance in the test animals. 

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How to Cite
Aldi, Y., Afriwardi, A., Badriyya, E., Azukhruf, W. S., & Alianta, A. A. (2023). Effects of Pegagan Embun (Hydrocotyle sibthorpioides Lam) Extract on Renal Function in Male Wistar Rats as Assessed by Creatinine Clearance: http://www.doi.org/10.26538/tjnpr/v7i3.13. Tropical Journal of Natural Product Research (TJNPR), 7(3), 2547–2550. Retrieved from https://tjnpr.org/index.php/home/article/view/1802
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References

Afriwardi A, Wahyuni F, Husni E, Alianta AA, Aldi Y. Effect of Standardised Extract PegaganEmbun

(Hydrocotylesibthorpioides Lam.) toward Natural Killer Cell and CD8 Cell Activities on White Male Mice Exposed to

H5N1 Virus Antigen. Open Access Maced J Med Sci. 2022 1;10(A):334-339.

Zhao L, Zhang N, Yang D, Yang M, Guo X, He J, Wu W, Ji B, Cheng Q, Zhou F. Protective effects of five structurally

diverse flavonoid subgroups against chronic alcohol-induced hepatic damage in a mouse model. Nutrients.

;10(11):1754.

Afriwardi, Aldi Y, Dillasamola D, Larakhansa YA, Badriyya E. Immunostimulatory activities of pegaganembun

(Hydrocotylesibthorpioides Lam.) in white male mice. Pharmacogn J. 2021;13(2):368-375.

Husni E, Dillasamola D, Badriyya E, Angelia R, Aldi Y. Ethanol extract activity of pegagan embun (Hydrocotylesibthorpioides Lam) against hematopoietic on anaemic male white mice. Pharmacogn J. 2021;13(4):866-

Afriwardi, Nazmi R, Dillasamola D, Badriyya E, Aldi Y. Anti-inflammatory activity of pegagan embun (Hydrocotylesibthorpioides Lam.) plant extract on topical application. PharmacognJ. 2021;13(2):411-417.

Afriwardi A, Wahyuni F, Husni E, Alianta AA, Aldi Y. Effect of standardised extract pegagan embun (Hydrocotylesibthorpioides Lam.) toward natural killer cell and CD8 cell activities on white male mice exposed to H5N1

virus antigen. Open Access Maced J Med Sci. 2022 1;10(A):334-339.

Aldi Y, Husni E, Yesika, R. Activity of kincung flowers (Etlingera Elatior (Jack) R.M.Sm.) on total leukocytes and

percentage of leukocytes in allergic wale White mice. Pharmacogn J. 2020; 12(1): 44-51.

Lee BM, Kacew S, Kim HS. Lu's Basic Toxicology: Fundamentals, Target Organs, and Risk Assessment. CRC Press; 2017 September 7.

Banasik JL. Pathophysiology-E-Book. Elsevier Health Sciences; 2018 January 17.

Departemen Kesehatan Republik Indonesia. Farmakope Herbal Indonesia. Edisi 2. Jakarta: Depkes RI; 2017.

Greiner A, Hildebrand J, Feltes R, Uter W, Drexler H, Göen T. Evaluation of urinary selenium as a biomarker of human

occupational exposure to elemental and inorganic selenium. Int Arch of Occup and Environ Health. 2020 Apr;93(3):325-

Hall JE, Hall ME. Guyton and Hall Textbook of Medical Physiology E-Book. Elsevier Health Sciences; 2020 June 13.

Swargiary A, Roy MK, Verma AK. In vitro study of the antioxidant, antiproliferative, and anthelmintic properties of some medicinal plants of Kokrajhar district, India. J of Parasit Dis. 2021 Dec;45(4):1123-1134.

Nurihardiyanti N, Yuliet Y, Ihwan I. Diuretic activities of combination of seed extract Carica Papaya L and Salacca

Zalacca Varietas Zalacca (Gaert.) Voss on Wistar strain male rats (Rattus norvegicus L.) or "Aktivitas Diuretik Kombinasi Ekstrak Biji Pepaya (Carica papaya L) dan Biji Salak (Salacca Zalacca Varietas Zalacca (Gaert.) Voss) pada Tikus Jantan Galur Wistar (Rattus Norvegicus L)”, Gale J of Pharm (e-Journal). 2015 Oct 1;1(2):105-112.

Yodoshi T, Arce-Clachar AC, Sun Q, Fei L, Bramlage K, Xanthakos SA, Flores F, Mouzaki M. Glomerular hyperfiltration is associated with liver disease severity in children with nonalcoholic fatty liver disease. The J of Paediatr. 2020 July 1;222:127-33.

Warditiani, NK, Sari PMNA, Yustiantara PS, Wirasuta, IMAG. Acute and sub-acute oral toxicity profile of purified

tomato extract on the liver and kidney functions of male Wistar rats. Trop J of Nat Prod Res. 2021. 5(11):1962-1965

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