In Vivo Evaluation of the Wound Healing Potential of the Aerial Parts of Globularia arabica Aqueous and Methanol Extracts Grown in Jordan http://www.doi.org/10.26538/tjnpr/v7i3.22
Article Sidebar
Main Article Content
Abstract
Globularia arabica is a wild, rare, and endangered medicinal plant that is used in folk medicine in Jordan. This study aimed to assess the wound healing potential, total phenol and flavonoid contents, antioxidant activity, and cytotoxic potential of G. arabica extracts that were extracted from the aerial parts. In vivo wound healing assays were performed using an excision wound model in rats. Animals were treated with a hydrogel formula enriched with plant extracts in two different concentrations (2% and 5%). The results demonstrated that wound healing activity was improved and statistically significant (p < 0.05) after topical application of both doses of 2% (w/v) and 5% (w/v) of methanolic (ME) and aqueous extracts (AE) of aerial parts of G. arabica. The total phenol and flavonoid contents were appreciably associated with the reductive capacities of both extracts. With respect to the DPPH, the IC50 was 26.54 mg/mL for AE, while for ME; it was 18.77 mg/mL, ME and AE were selectively noncytotoxic on PDL fibroblasts.ME was exceptionally more effective than AE, exhibiting pronounced viability reduction propensities for aggressively resistant CACO2 cancer cells. While AE was ascribed more substantial growth inhibition properties in T47D breast adenocarcinoma. In conclusion, the study revealed that the ME and AE of G. arabica displayed remarkable wound healing activity, corroborating its traditional use. Moreover, phytochemical fingerprinting of both crude extracts can associate G. arabica'smaximal biological and
pharmacological effects with bioactive metabolites.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Frišcic M, Maslo S, Garic R, Maleš Ž, Pilepic KH. Comparative analysis of specialized metabolites and antioxidant capacity in vitro of different natural populations of Globularia spp. Acta Bot. Croat. 2018; 77(1): 1–9.
Brindisi M, Bouzidi C, Frattaruolo L., Loizzo MR, Tundis R, Dugay A, Deguin B, Cappello AR, Cappello MS. Chemical profile, antioxidant, anti-inflammatory, and anticancer effects of Italian Salvia rosmarinusSpenn. methanol leaves extracts. Antioxidants. 2020; 9(9): 1–21.
El Abed H, Chakroun M, Abdelkafi-Koubaa Z, Drira N, Marrakchi N, Mejdoub H, Khemakhem B. Antioxidant, anti-Inflammatory, and antitumoral effects of aqueous ethanolic extract from Phoenix dactylifera L. parthenocarpic dates. BioMed Res. Int. 2018; 1–8.
Al-Qaraleh, S. Y., Al-Zereini, W. A., Oran, S. A., AlSarayreh, A. Z., &Sa'ed, M. (2022). Evaluation of the antioxidant activities of green synthesized selenium nanoparticles and their conjugated polyethylene glycol (PEG) form in vivo. OpenNano, 8, 100109.
Khleifat, K., Magharbeh, M., Alqaraleh, M., Al-Sarayrah, M., Alfarrayeh, I., Al Qaisi, Y., Ahmad Alsarayreh d, Mohammad A. Al-kafaween. (2022). Biodegradation modeling of phenol using Curtobacteriumflaccumfaciens as plant-growth-promoting bacteria. Heliyon, 8(9), e10490.
Frankova A, Vistejnova L, Merinas-Amo T, Leheckova Z, Doskocil I, Wong Soon J, Kudera T, Laupua F, AlonsoMoraga A, Kokoska L. In vitro antibacterial activity of extracts from Samoan medicinal plants and their effect on proliferation and migration of human fibroblasts. J. Ethnopharmacol.. 2021; 264: 113220.
Oran SA. The status of medicinal plants in Jordan. JAST. 2014; 4: 461-467.
Al-Eisawi DM. List of Jordan vascular plants. Mitt. Bot. Munchen. 1982; 18: 79–182.
Oran SA, Al-Eisawi DM. Check-list of medicinal plants in Jordan. Dirasat. 1998; 25(2): 84–112.
Oran SA, Raies AM. Antimicrobial activity of Globularia arabicaJaub. and Spach and G. alypum L. (Plantaginaceae). Dirasat. 2002; 27(1): 71–73.
Oran SA. Potato disc bioassay for some Jordanian medicinal plants. Pharm. Biol. 1999; 37(4): 296–299.
Al-Soub, A., Khleifat, K., Al-Tarawneh, A., Al-Limoun, M., Alfarrayeh, I., Al Sarayreh, AYaseen Al Qaisi1, HaithamQaralleh,MoathAlqaraleh,AnasAlbashaireh. (2022). Silver nanoparticles biosynthesis using an airborne
fungal isolate, Aspergillus flavus: optimization, characterization and antibacterial activity. Iranian Journal of Microbiology, 14(4).
Jaffal, SM, Oran SA, Alsalem MI. Anti-inflammatory and antipyretic potential of Arbutus andrachneL . methanolic
leaf extract in rats. Asian Pac. J. Trop. Biomed. 2021; 11(11): 491–499.
Al Qaisi, Y. T., Khleifat, K. M., Alfarrayeh, I. I., &Alsarayreh, A. Z. (2022). In Vivo Therapeutic Effect of Some Medicinal Plants’ Methanolic Extracts on the Growth and Development of Secondary Hydatid Cyst Infection. Acta Parasitologica, 1-14.
Stankovi MS. Total phenolic content , flavonoid concentration and antioxidant activity of Marrubiumperegrinum L . extracts. Kragujevac J Sci. 2011; 33: 63–72.
Bouyahya A, Dakka N, Talbaoui A, El Moussaoui N, Abrini J, Bakri Y. Phenolic contents and antiradical capacity of vegetable oil from Pistacialentiscus (L). J. Mater. Environ. Sci. 2018; 9(5): 1518–1524.
Mariem C, Sameh M, Nadhem S, Soumaya Z, Najiba Z, Raoudha EG. Antioxidant and antimicrobial properties of the extracts from Nitrariaretusa fruits and their applications to meat product preservation. Ind Crops Prod. 2014; 55: 295–303.
Althaher AR, Oran SA, Bustanji YK. Chemical composition, In vitro evaluation of antioxidant properties and cytotoxic activity of the essential oil from Calaminthaincana (Sm.) Helder (Lamiaceae). TJNPR. 2021; 5(8): 1333–1339.
Khleifat, K., Alqaraleh, M., Al-limoun, M., Alfarrayeh, I., Khatib, R., Qaralleh, H., Ahmad Alsarayreh, Yaseen Al
Qaisi, Maha Abu Hajleh. (2022). The ability of rhizopusstolonifer MR11 to biosynthesize silver nanoparticles in response to various culture mediacomponents and optimization of process parameters required at each stage of biosynthesis. Journal of Ecological Engineering, 23(8).
Bustanji Y, AlDouri N, Issa A, Mashallah S, Assaf A, Aburjai T, Mohammad M. Cytotoxic effect of Mercurialisannua L. methanolic extract on six human solid cancer cell lines. SRE. 2012; 7(37): 3218–3222.
Peng L, Wang B, Ren P. Reduction of MTT by flavonoids in the absence of cells. Colloids Surf. B. 2005; 45(2 ): 108–111.
Alsarayreh AZA, Oran SA, Shakhanbeh JM. Effect of Rhuscoriaria L. methanolic fruit extract on wound healing in diabetic and non-diabetic rats. J. Cosmet. Dermatol. 2022; 21(8): 3567-3577.
Barka ZB, Tlili M, Alimi H, Miled HB, Rhouma KB, Sakly M, Ksouri R, Schneider YJ, Tebourbi O. Protective effects of edible Rhustripartita (Ucria) stem extract against nol-induced gastric ulcer in rats. J Funct Foods. 2017; 1(30): 260-269.
Khleifat KM, Abboud M, Omar S, Al-Kurishy J. Urinary tract infection in South Jordanian population. J Med Sci.
; 6(1): 5-11.
Khleifat KM, Abboud MM, Al-Mustafa AH, Al-Sharafa KY. Effects of carbon source and Vitreoscilla hemoglobin (VHb) on the production of β-galactosidase in Enterobacter aerogenes. CurrMicrobiol. 2006; 53(4): 277-281.
Abboud MM, Aljundi IH, Khleifat KM, Dmour S. Biodegradation kinetics and modeling of whey lactose by bacterial hemoglobin VHb-expressing Escherichia coli strain. BiochemEng J. 2010; 48(2): 166-172.
Hossain MA, Shah MD, Gnanaraj C, Iqbal M. In vitro total phenolics, flavonoids contents and antioxidant activity of essential oil, various organic extracts from the leaves of tropical medicinal plant Tetrastigma from Sabah. Asian Pac
J Trop Med. 2011; 4(9): 717- 721.
Althunibat OY, Qaralleh H, Al-Dalin SYA, Abboud M, Khleifat K, Majali IS, Aldal'in H, Rayyan WA, Jaafraa A. Effect of thymol and carvacrol, the major components of Thymus capitatus on the growth of Pseudomonas aeruginosa. J Pure Appl Microbiol. 2016; 10(1): 367-374.
Al-Asoufi A, Khlaifat A, Tarawneh A, Alsharafa K, AlLimoun M, KhleifatK. Bacterial quality of urinary tract infections in diabetic and non-diabetics of the population of Ma'an Province, Jordan. Pak J Biol Sci. 2017; 20(4):179- 188.
Alsarayreh AZA, Oran, SA, Shakhanbeh JM. In vitro and in vivo wound healing activities of Globularia arabica leaf
methanolic extract in diabetic rats. J. Cosmet. Dermatol. 2022; 21(10): 4888-900.
Al-Sarayreh AZ, Khleifat KM, Al-Dalain SEM, Al-Saraireh YM, Al-Qaisi YT, Al-Farrayeh II, Al-Qaraleh SY. Globularia arabica methanolic leaf extract has higher efficacy on burn wound healing in diabetic rats compared to Malvaslyvestriesmethanolic leaf extract. J Burn Care Res. 2022; irac089-irac089.
Es-Safi N-E, Kollmann A, Khlifi S, Ducrot P-H. Antioxidative effect of compounds isolated from Globulariaalypum L. structure activity relationship. LWTFood Sci Technol. 2007; 40(7): 1246-1252.
Al-Saraireh, Y. M., Youssef, A. M., Alsarayreh, A. Z., Al Hujran, T. A., Al-Sarayreh, S., Al-Shuneigat, J. M., &Alrawashdeh, H. M. (2021). Phytochemical and anticancer properties of Euphorbia hierosolymitanaBoiss. crude extracts. J. Pharm. Pharmacogn. Res, 9, 13-23.
Alfarrayeh, I., Aloshoush, A., Ahmad, A., & Al Qaisi, Y. (2022). Survey of medicinal plants sold in local markets in Ghor As-Safi, Southern Jordan. Medicinal Plants International Journal of Phytomedicines and Related Industries, 14(2), 340-343.
Harzallah HJ, Neffati A, Skandrani I, Maaloul E, ChekirGhedira L, Mahjoub T. Antioxidant and antigenotoxic activities of Globulariaalypum leaves extracts. J. Med. Plant Res. 2010; 4(19): 2048-2053.
Mansour RB, Gargouri B, Gargouri B, Elloumi N, Jilani IB, Ghrabi-Gammar Z, Lassoued S. Investigation of antioxidant activity of alcoholic extract of Globulariaalypum L. J Med Plants Res. 2012; 6: 4193-4199.
Taghzouti OK, Balouiri M, Ouedrhiric W, Chahadd AE, Romanea A. In vitro evaluation of the antioxidant and antimicrobial effects of Globulariaalypum L. extracts. J. Mater. Environ. Sci. 2016; 7: 1988-1995.
Alsarayreh AZ, Oran SA, Shakhanbeh JM, Khleifat KM, Al Qaisi YT, Alfarrayeh II, Alkaramseh AM. Efficacy of methanolic extracts of some medicinal plants on wound healing in diabetic rats. Heliyon. 2022; 8(8): e10071.