Evaluation of the Antioxidant and Hypoglycaemic Potentials of the Leaf Extracts of Stachytarphyta jamaicensis (Verbenaceae)
doi.org/10.26538/tjnpr/v3i5.4
Keywords:
Antioxidants, Free radicals, Herbal medicines, Diabetes, Stachytarpheta jamaicensisAbstract
Stachytarpheta jamaicensis (family Verbanaceae) commonly known as blue porterweed is widely used in folk medicine for the treatment of various diseases including diabetes mellitus. The present study investigated the blood glucose lowering effect and the antioxidant activity of the leaf extracts of Stachytarpheta jamaicensis. The phytochemical screening of the powdered leaf sample was done according to standard procedures. The antioxidant activity of the methanol and ethyl acetate extracts of the leaves were investigated using the 1,1-diphenyl-2-picrylhydrazine (DPPH) radical scavenging assay, the total phenolic and flavonoid contents were also evaluated following standard procedures. The hypoglycaemic effect of the extracts was evaluated using streptozotocin-induced diabetes in rats. Phytochemical screening shows the presence of carbohydrates, alkaloids, saponins, tannins, phenolic compounds, and flavonoid The exracts demonstrated appreciable and concentration-dependent radical scavenging effect with IC50 values of 16.95µg/mL and 33.12µg/mL, for the methanol extract and ethyl acetate extract, respectively. Oral administration of the extracts at 200mg/kg and 400mg/kg daily dose significant lowers blood glucose levels instreptozotocin-induced diabetes in experimental rats compared to the control group (untreated diabetic animals). The present findings have therefore shown that S. jamaicensis leaves has hypoglycaemic and antioxidant effects and may therefore serve as a potential source of hypoglycaemic agent as well as antioxidant agents for the prevention and management of free radical induced metabolic diseases.
References
Craig, CR and Stitzel RE. Modern pharmacology with clinical applications. Boston: Little, Brown. 1997.
Harris MI, Klein R, Welborn TA, Knuiman MW. Onset of NIDDM occurs at least 4-7 yr before clinical diagnosis. Diabet Care. 1992; 15(7):815-819.
Kumar PJ and Clark M. Diabetes mellitus and other disorders of metabolism. Clin Med. 2002; 2:1069-1121.
Haque N, Salma U, Nurunnabi TR, Uddin MJ, Jahangir MFK, Islam SMZ, Kamruzzaman M. Management of Type 2 Diabetes Mellitus by Lifestyle, Diet and Medicinal Plants. Pak J Bio Sci. 2011; 14(1):13-24.
Farnsworth NR and Soejarto DD. Potential consequences of plant extinction in the united states on the current and future availability of prescription drugs. J Econ Bot 1985; 39(3):231-240.
Sofowora A. Medicinal Plants and Traditional Medicine in Africa. 24th Edn., John Wiley and Sons, New York, USA., 1992. 8 p.
Falodun A. Herbal Medicine in Africa-Distribution, Standardization and Prospects. Research Journal of Phytochemistry. 2010; 4(3): 154-161.
Jagadish NRN and Gopalkrishna B. “Evaluation of analgesic activity of different extracts of Stachytarpheta indica L. (Vahl).” Biomed. 2008; 3(3-4):229–233.
Sulaiman MR, Zakaria ZA, Chiong HS, Lai SK, Israf DA, Azam Shah TM. “Antinociceptive and anti-inflammatory effects of Stachytarpheta jamaicensis (L.) Vahl (Verbenaceae)in experimental animal models.” Med Prin Prac. 2009; 18(4):272–279.
Valdés AF-C, Martínez JM, Lizama RS, Gaitén YG, Rodríguez DA, Payrol JA. “In vitro antimalarial activity and cytotoxicity of some selected cuban medicinal plants,” Revista do Instituto de Medicina Tropical de São Paulo 2010; 52(4):197–201.
Idu M, Omogbai EKI, Aghimien GE, Amaechina F, Timothy O, Omonigho SE. “Preliminary phytochemistry, antimicrobial properties and acute toxicity of Stachytarpheta jamaicensis (L.) Vahl. leaves,” Trends Med Res. 2007; 2(4):193–198.
Ataman JE, Idu M, Odia EA, Omogbai EKI, Amaechina F, Akhigbe AO, Ebite LE. “Histopathologic effects of Stachytarpheta jamaicensis (L.) Vahl on Wistar rats,” Pak J Bio Sci. 2006; 9(3):477–482.
Stalh E. Drug analysis by chromatography and Microscopy. A Practical Supplement to Pharmacopoeias, 1st ed., Ann Arbor, Michigan: 1973. 219-224 p.
Sofowora A. Screening Plants for Bioactive Agents. In Medicinal Plants and Traditional Medicine in Africa. Spectrum Books Ltd., Ibadan: 1982. 128-161 p.
Jain A, Soni M, Deb L, Jain A, Rout S, Gupta V, Krishna K. Antioxidant and hepatoprotective activity of ethanolic and aqueous extracts of Momordica dioica Roxb. leaves. J Ethnopharmacol. 2008; 115(1):61-66.
Kim DO, Jeong SW, Lee CY. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem. 2003; 81:321-326.
Ebrahimzadeh MA, Pourmorad F, Bekhradnia AR. Iron chelating activity, phenol and flavonoid content of some medicinal plants from Iran. Afr J Biotechnol. 2008; 7(18):3188-3192.
National Institutes of Health (NIH) Publication (1985). Guide for the Care and Use of Laboratory Animals. No. 85-23.
Liew PM and Yong YK. “Stachytarpheta jamaicensis (L.) Vahl: From Traditional Usage to Pharmacological Evidence”. Evid-Based Compl Altern Med. 2016, Article ID 7842340, 7 p.
Prior RL, Wu X, Schaich K. Standardized Methods for the Determination of Antioxidant Capacity and Phenolics in Foods and Dietary Supplements. Journal of Agriculture and Food Chem. 2005; 53:4290-4302.
Horax R, Hettiarachchy N, Islam S. Total Phenolic contents and phenolic acid constituents in 4 varieties of bitter melons (Momordica charantia) and antioxidant activities of their extracts. J Food Sci. 2005; 70(4):275-280.
Sathishsekar D and Subramanian S. Antioxidant properties of Momordica Charantia (bitter gourd) seeds on Streptozotocin induced diabetic rats. Asia Pac J Clin Nutr. 2005; 14(2):153-158.
Elmastas M, Isildak O, Turkekul I, Temur N. Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms. J Food Comp Anal. 2007; 20:337-345.
Afanas’ev P. Chelating and free radical scavenging mechanisms of inhibitory action of rutin and quercetin in lipid peroxidation. Biochem Pharmacol. 1989; 38:1763-1769.
Amarowicz R, Pegg RB, Rahimi-Moghaddam P, Barl B, Weil JA. Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food Chem. 2004; 84:551-562.
Pietta P. Flavonoids as antioxidants. J Nat Prod. 2000; 63(7):1035-1042.
Nijveldt RJ, van Nood E, van Hoorn DEC, Boelens PG, van Norren K, van Leeuwen PA. Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr. 2001; 74(4):418-425.
Russo A, Acquaviva R, Campisi A, Sorrenti V, Di Giacomo C, Virgata G, Barcellona MI, Vanella A. Bioflavonoids as antiradicals, antioxidants and DNA cleavage protectors. Cell Bio Toxicol. 2000; 16(2):91.
Ibrahim SS. Protective effect of hesperidin, a citrus bioflavonoid, on diabetes-induced brain damage in rats. J Appl Sci Res. 2008; 4(1):84
King H, Aubert RE and Herman WH. Global burden of diabetes, 1995-2025: prevalence, numerical estimates, and projections. Diabet Care 1998; 21:1414–1431.
Balogun FO, Tshabalala NT, Ashafa AO. Antidiabetic Medicinal Plants Used by the Basotho Tribe of Eastern Free State: A Review. J Diabet Res. 2016, Article ID 4602820, 13 p.
Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res. 2001; 50:537-546.
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