The Potential of Lampung Robusta Green Coffee (Coffea Canephora) Extract Toward T Cell Activation in ISA-brown Laying Chickens
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Abstract
Coffee (Coffea canephora) is one of the world's most popular beverages containing active chemicals such as caffeine, phenol, trigonelline, diterpenes, and water-soluble substances. Chlorogenic acids are a type of phenol found in coffee that can stimulate the immune system within the body. As a result, this natural substance promotes poultry growth by strengthening the immune system and preventing disease. There is a dearth of information on coffee as an immunomodulator in chickens, necessitating this investigation. The present study was therefore conducted to investigate the effect of Lampung Robusta green coffee extract on T lymphocyte cell activation in ISA-brown laying chickens. Ethanolic extract of the Lampung Robusta green coffee was prepared and subjected to liquid chromatography-mass spectrometric analysis. One-day-old ISA-brown laying chickens were divided into four groups: control (C), T1, T2, and T3 groups, which received extract coffee doses of 500, 1000, and 1500 mg/kg BW, respectively. The relative numbers of TCD4+ , CD8+ , and CD45+ cells were determined using a flow cytometer. The results of the LC-MS analysis revealed that the Lampung Robusta green coffee extract also contained chlorogenic acids. With a dose of 1500 mg/kg BW, Lampung Robusta green coffee extract had an immunomodulatory effect on the T3 group, increasing the relative number of TCD4+ and CD45+ while decreasing CD8+ . The relative number of TCD8+ was higher than that of TCD4+ , suggesting immunosuppressive potential. The findings of this study revealed that Lampung Robusta green coffee extract is effective as an immunomodulator in chickens.
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Martini D, Del Bo C, Tassotti M, Riso P, Del Rio D, Brighenti F, Porrini M. Coffee consumption and oxidative stress: A review of human intervention studies. Mol. 2016; 21(8):979.
O’Gorman M. Flow Cytometry Assay in Primary Immunodeficiency Disease. Springer Sci. 2018; 1678:321-345.
Monteiro M, Farah A, Perrone D, Trugo LC, Donangelo C. Chlorogenic acid compounds from coffee are differentially absorbed and metabolized in humans. J Nutr. 2007; 137(10):2196-2201.
Qosimah D, Rosyidi D, Radiati LE, Amri IA, Prasetyo D, Permata FS, Beltran MAG, Aulanni’am A, Annisa AK. Immunomodulator effect of Robusta Lampung coffee extract (Coffee Canephora Var Robusta) in layer chicken infected with Salmonella enteritidis bacteria. J Ilmu-Ilmu
Peternak. 2020; 30(1):69-79.
Widada W. Pengaruh Bekam Terhadap Peningkatan Sel T CD8 Sebagai Mekanisme Pertahanan Tubuh. Jurnal Penelitian Kesehatan Suara Forikes 2011; 4:
Pinca SP, Djati MS, Rifa’i M. Analisis Mobilisasi Sel T CD4+ dan CD8+ pada Timus Ayam Pedaging Pasca Infeksi Salmonella typhimurium dan Pemberian Simplisia Polyscias obtusa. Biotropika J Trop. 2013; 1(1):27-32.
Samoggia A and Riedel B. Consumers’ perceptions of coffee health benefits and motives for coffee consumption and purchasing. Nutr. 2019; 11(3):653
Varol C, Mildner A, Jung S. Macrophages : Development and Tissue Specialization. Annu Rev Immunol. 2015; 33:643-675
Febrianty H and Djati M. Modulasi sel T CD4 dan CD8 pada Spleen Ayam Arab Putih (Gallus turcicus) dengan Ransum yang mengandung Daun Pepaya (Carcica papayaL.). J Biotropika. 2015; 3(3):107-111.
Abbas A, Litchman A, Pillai S. Basic Immunology: Function and Disorders of the Immune Systeme. In: 5th ed. Elsivier Inc; 2000.
Akrom, Widjaya A, Armansyah T. Ethanolic Extract of Black Cumin (Nigella sativa) Seed Increases Macrophage Phagocytic Activity of Swiss Mice Infected with Lysteria monocytogenes. J Kedokt Hewan. 2015; 9(2):94-100.
Chu YF, Chen Y, Brown PH, Lyle BJ, Black RM, Cheng IH, Ou B, Prior RL Bioactivities of crude caffeine: Antioxidant activity, cyclooxygenase-2 inhibition, and enhanced glucose uptake. Food Chem. 2012; 131(2):564-568.
Hermiston ML, Xu Z, Weiss A. CD45: A critical regulator of signaling thresholds in immune cells. Annu Rev Immunol. 2003; 21:107-137.
Ding S, Jiang H, Fang J. Regulation of immune function by polyphenols. J Immunol Res. 2018; 2018:Article ID 1264074, 8 pages