Toxicity Study on the Effect of Ethanol Extracts of the Root and Leaf of Datura stramonium on the Brain and Non-Prostatic Acid Phosphatase Activity of Wistar rats
http://.www.doi.org/10.26538/tjnpr/v7i2.25
Keywords:
brain, histology, non-prostatic acid phosphatase, Datura stramoniumAbstract
Abuse of psychoactive compounds is a growing global challenge, especially in countries with high unemployment rates. Toxicity study was conducted on the effect of ethanol extracts of the root and leaf of a regularly abused psychoactive plant in Nigeria (Datura stramonium) on the brain and non-prostatic acid phosphatase activity of Wistar rats using sixteen (16) Wistar rats of average weights 135g that were assigned into four (4) groups A, B, C and D with four (4) rats each. Groups A, B, and C were given the same concentration (3.5% in chilled Coca-Cola) of root extracts, leaf extracts, and both leaf and root extracts respectively while group D (control) was administered with equal volume of chilled Coca-Cola drink. The animals were kept in separate cages and fed with normal rat Chaw with water provided ad libitum. Rats in the treatment groups were given approximately 3.7mg/Kg body weight of the root extract, leaf extract, and both leaf and root extracts respectively while rats in the control group were given just a chilled Coca-Cola drink. All the animals were sacrificed after day 14th of administration. A qualitative phytochemical screening on the ethanol extracts of the root and leaf of D. stramonium plant showed that it contains alkaloids, tannins, saponins, flavonoids, and steroids. Histological examination of the brain of rats administered ethanol extracts of the root and leaf of the plant showed some levels of toxicity (exemplified by shrinkage of nerve cells) as well as increased activity of serum level of non prostatic acid phosphatase.
References
Guidance document on physical Datura stramonium seed contamination. Rome. [Internet]. 2020. Available from: https://doi.org/10.4060/cb2105en.
Berkov S, Zayed R, Doncheva T. Alkaloid patterns in some varieties of Datura stramonium. Fitoterapia. 2006;77(3):179-82.
Benítez G, March-Salas M, Villa-Kamel A, Cháves-Jiménez U, Hernández J, Montes-Osuna N, Moreno-Chocano J, Cariñanos P. The genus Datura L.(Solanaceae) in Mexico and Spain–Ethnobotanical perspective at the interface of medical and illicit uses. Journal of ethnopharmacology. 2018;219:133-51.
Al-Snafi, Ali E. Traditional uses of Iraqi medicinal plants. IOSR Journal of Pharmacy. 2018;8(8):32-96.
Soja S, Saradha M. Documentation of medicinal plants used by the traditional healers, mayannur forest, thrissur district, kerala, india. Kongunadu Research Journal. 2021;8(2):8-26.
Peredery O, Persinger MA. Herbal treatment following post‐seizure induction in rat by lithium pilocarpine: Scutellaria lateriflora (Skullcap), Gelsemium sempervirens(Gelsemium) and Datura stramonium (Jimson Weed) may prevent development of spontaneous seizures. Phytotherapy
Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 2004;18(9):700-5.
Mukhtar Y, Tukur S, Bashir R. An Overview on Datura stramonium L.(Jimson weed): A Notable Psychoactive Drug Plant. American Journal of Natural Sciences. 2019;2(1):1-9.
Das S, Kumar P, Basu S. Phytoconstituents and therapeutic potentials of Datura stramonium Linn. Journal of Drug Delivery and Therapeutics. 2012;2(3):4-7.
Oseni O, Olarinoye C, Amoo I. Studies on chemical compositions and functional properties of thorn apple (Datura stramonium L) Solanaceae. African Journal of Food Science. 2011;5(2):40-4.
Shagal M, Modibbo U, Liman A. Pharmacological justification for the ethnomedical use of Datura stramoniumstem-bark extract in treatment of diseases caused by some pathogenic bacteria. Int Res Pharm Pharmaco. 2012;2(1):16-9.
Sanni M, Ejembi D, Abbah O, Olajide J, Ayeni G, Asoegwu C. Studies on the phytochemical screening and deleterious effects of aqueous extract of Datura stramonium on albino rats. World Journal of Pharmaceutical research. 2014;3(6):1110-36.
Arifin WN, Zahiruddin WM. Sample Size Calculation in Animal Studies Using Resource Equation Approach. Malays J Med Sci. 2017;24(5):101-5.
Parasuraman S, Raveendran R, Kesavan R. Blood sample collection in small laboratory animals. Journal of pharmacology & pharmacotherapeutics. 2010;1(2):87.
Miteva R, Zapryanova D, Fasulkov I, Yotov S, Mircheva T. Investigations on acid phosphatase activity in the seminal plasma of humans and animals. Trakia J Sci. 2010;8:20-3.
Halpern JH. Hallucinogens and dissociative agents naturally growing in the United States. Pharmacology & therapeutics. 2004;102(2):131-8.
Rodrigues D, Li A, Nair D, Blennerhassett M. Glial cell line‐derived neurotrophic factor is a key neurotrophin in the postnatal enteric nervous system. Neurogastroenterology & Motility. 2011;23(2):e44-e56.
Glatstein M, Alabdulrazzaq F, Scolnik D. Belladonna alkaloid intoxication: the 10-year experience of a large tertiary care pediatric hospital. American journal of therapeutics. 2016;23(1):e74-e7.
Ademiluyi AO, Ogunsuyi OB, Oboh G, Agbebi OJ. Jimson weed (Datura stramonium L.) alkaloid extracts modulate cholinesterase and monoamine oxidase activities in vitro: possible modulatory effect on neuronal function. Comparative Clinical Pathology. 2016;25(4):733-41.
Fasakin OW, Oboh G, Ademosun AO, Lawal AO. The modulatory effects of alkaloid extracts of Cannabis sativa, Datura stramonium, Nicotiana tabacum and male Carica papaya on neurotransmitter, neurotrophic and neuroinflammatory systems linked to anxiety and depression. Inflammopharmacology. 2022;30:2447-76.
Fasakin OW, Oboh G, Ademosun AO. Neuromodulatory evaluation of commonly abused plants ex vivo: a comparative study. Comparative Clinical Pathology. 2021;30(4):671-80.
Marisco PC, Carvalho FB, Rosa MM, Girardi BA, Gutierres JM, Jaques JA, Salla AP, Pimentel VC, Schetinger MRC, Leal DB. Piracetam prevents scopolamine-induced memory impairment and decrease of NTPDase, 5′-nucleotidase and adenosine deaminase activities. Neurochemical research.
;38(8):1704-14.
Peter Etim Ekanem, Dare S. Sunday, Abba S, Tesfay A. Hippocampal pyramidal cell degeneration and changes in CREB protein in Datura stramonium treated rats. American Journal of Neuroscience. 2015;6(2):13-9.
Ogunmoyole T, Adeyeye RI, Olatilu BO, Akande OA, Agunbiade OJ. Multiple organ toxicity of Datura stramonium seed extracts. Toxicology reports. 2019;6:983-9.
Wang X, Wang W, Li L, Perry G, Lee H-g, Zhu X. Oxidative stress and mitochondrial dysfunction in Alzheimer's disease. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease. 2014;1842(8):1240-7.
Kellett KA, Hooper NM. The Role of Tissue Non-specific Alkaline Phosphatase (TNAP) in Neurodegenerative Diseases: Alzheimer's Disease in the Focus. Subcell Biochem. 2015;76:363-74.
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