Evaluation of the Anti-Inflammatory, Antioxidant, and Protease Inhibitory Activity of the Crude Methanol Extract of Portulaca oleracea
http://.www.doi.org/10.26538/tjnpr/v7i2.15
Keywords:
Plant extracts, Antioxidant, Anti-inflammatory, Portulaca oleracea, Protease inhibitorsAbstract
Protease inhibitors have considerable applications in biomedicine, biotechnology, and therapy. The aim of the study was to evaluate the methanol crude extracts of aerial portions of Portulaca oleracea (P. oleracea) for anti-inflammatory, antioxidant, and protease inhibitor activities, and to evaluate their inhibitory action against various therapeutically significant proteases. A modified colorimetric assay was used to analyze the protease inhibitors on trypsin and other therapeutic enzymes. P. oleracea crude leaf extract exhibited 79.92% antioxidant and 75% anti-inflammatory activity. Analysis of the effects of crude P. oleracea extract on the values of enzyme kinetics (Km, Vmax) was determined by Lineweaver-Burk reciprocal plot. It was found that the extract exhibited a potent inhibitory effect on the Vmax (µmole/min) of the chymotrypsin 26.0, trypsin 18.0, papain 8.33, and elastase 10.0, when compared to control 41.0, 73.0, 11.11, and 20.3, respectively. While no effect on Km except for elastase which was decreased from 0.026 mM for the control to 0.01 mM. It was found that this effect showed a powerful inhibition in a different way. There is a mixed non-competitive inhibition on elastase as well as pure non-competitive inhibition on chymotrypsin, trypsin, and papain. The crude extract contained 53.3%, 55.7%, 14.9%, and 26.9%, respectively, of protease inhibitors for the therapeutic protease enzymes trypsin, papain, chymotrypsin, and elastase. Therefore, trypsin, papain, chymotrypsin, and elastase have been inhibited by the crude extract of P. oleracea. Characterization of the crude extract indicated potent anti-inflammatory and antioxidant effects.
References
Shahin R, Javad Z. Post-translational modifications in proteins: resources, tools and prediction methods. Database. 2021; 12: 342–350
Seukep A, Kuete V, Nahar L, Sharker SD, Guo M. Plantderived secondary metabolites as the main source of efflux pump inhibitors and methods for identification. J Pharm Anal. 2020;10(4):277-290.
Singh KR, Nayak V, Singh J, Singh AK, Singh RP. Potentialities of bioinspired metal and metal oxide nanoparticles in biomedical sciences. RSC advances. 2021.; 11(40):24722-24746.
Zhu-Salzman, K., Zeng R. Insect response to plant defensive protease inhibitors. Annu. Rev. Entomol. 2015; 60(1): 233-252.
Orhan, I. E. Enzyme inhibitors are attractive targets for treating various diseases. Curr. Med. Chem.2019; 26(18):3206-3207.
Hordofa, T. G., & Kiros, T. Potential use of Eastern Hararghe Ethiopia medicinal plants for COVID-19-like symptoms: A review. AJMAP. 2020; 6(3):76-115.
Xu J, Miao H, Zou L, Tse Sum B, Haupt K, Pan G. Evolution of molecularly imprinted enzyme inhibitors: from simple activity inhibition to pathological cell regulation. Angew. Chem. 2021;133(46): 24731-24738.
Westby MJ, Norman G, Watson RE, Cullum NA, DumvilleJC. Protease activity as a prognostic factor for wound healing in complex wounds. Wound Repair Regen. 2020;28(5):631-644.
Tsobou R, Hamawa Y, Fawa G, Simplice J, Marie L, Wouokoue T, Mapongmetsem P, Van Damme P. Can antimalarial, antiviral, and anti-respiratory infections Cameroonian medicinal plants be used as one of the potential ways to cure COVID-19? Pharmacological and ethnomedicinal proof. J. med. herbs ethnomed. 2020; 6: 61-85.
María G, Rosalía L, Paula H, Francisco G, Fernando G. Scaled‐up biotechnological production of individual betalains in a microbial system Microb Biotechno. 2019; 12(5): 993–1002.
Patel SR. Studies for Developing Shoot Cultures of Leptadenia reticulata (Retz.) Wight and Arn and Tylophora indica (Burm. F.) Merrill with Phytochemical Analysis. Maharaja Sayajirao University of Baroda (India). 2021.
Hellinger R, Gruber CW. Peptide-based protease inhibitors from plants. Drug Discov. Today. 2019; 24(9): 1877-1889.
Mandal A, Jha AK, Hazra B. Plant products as inhibitors of coronavirus 3CL protease. Front. Pharmacol.2021: 12: 583387.
Sadiq ME, Code Q. Bactericidal effects of 8, 9-dihydroxy-1, 5, 6, 10b-tetrahydropyrrolo [2, 1-a] isoquinolin-3 (2H)-one (Trolline) on selected entero-pathogenic bacteria. IJPR. 2017; 7(10):203–207
Mohammed MT, Kadhim SM, AL-Qaisi ZH. Positive influence of Portulaca oleracea L. in rats with type 2 diabetes mellitus. Plant Arch. 2020; 20(2): 893-897.
Echecopar-Sabogal J, D’Angelo-Piaggio L, Chanamé-Baca DM, Ugarte-Gil C. Association between the use of protease inhibitors in highly active antiretroviral therapy and incidence of diabetes mellitus and/or metabolic syndrome in HIV-infected patients: A systematic review and metaanalysis. Int. J. STD AIDS. 2018;29(5):443-452.
Allegra M. Antioxidant and anti-inflammatory properties of plants extract. Antioxidant.2019; 8: 549.
Atrooz OM, Al-Maitah SZ. Characterization of the crude extract of Portulaca oleracea and the determination of the polyphenol oxidase kinetics in the presence of Cu and Zn. J. appl. biol. biotechnol. 2022; 10(3): 2-3.
Memarzia A, Khazdair MR, Behrouz S, Gholamnezhad Z, Jafarnezhad M, Saadat S, Boskabady MH. Experimental and clinical reports on anti‐inflammatory, antioxidant, and immunomodulatory effects of Curcuma longa and curcumin, an updated and comprehensive review. BioFactors. 2021; 47(3):311-350.
Elisha IL, Dzoyem JP, McGaw LJ, Botha FS, Eloff JN. The anti-arthritic, anti-inflammatory, antioxidant activity and relationships with total phenolics and total flavonoids of nine South African plants used traditionally to treat arthritis. BMC Complement Altern Med. 2016; 16(1):1-10.
Alcántara C, Žugčić T, Abdelkebir R, García-Pérez JV, Jambrak AR, Lorenzo JM, Barba F J. Effects of ultrasoundassisted extraction and solvent on the phenolic profile, bacterial growth, and anti-inflammatory/antioxidant activities of mediterranean olive and fig leaves extracts.
Molecules. 2020; 25(7):1718-1729
Sivakumar S, Prabha D, Velmurugan P, Hong S, Yi P, Jang S, Suh J. Phytoremediation of Cu and Cd-contaminated roadside soils by using stem cuttings of Portulaca oleracea L. J. Environ. Chem. Ecotoxicol. 2020; 2:201-204.
Zhao L, Feng C, Wu K, Chen W, Chen Y, Hao X, Wu, Y. Advances and prospects in biogenic substances against plant virus: A review. PESTIC BIOCHEM PHYS. 2017; 135: 15-26.
Shahnaz M, Khan B, Sardar K, Mian I , Muhammad M. Contaminated and bioaccumulation of heavy metals in medicinal plants of district Dir Upper ,khyper Pakhunkhwa, Pakistan. Pak. J. Bot. 2021;53(6):2179-2186
Alamgeer Niazi SG, Uttra AM, Qaiser MN, Ahsan H. Appraisal of anti-arthritic and nephroprotective potential of Cuscuta reflexa. Pharm Biol.2017; 55(1), 792-798.
Blandine M L, Serge N.E, Clergy T. Partial Purification and Characterization of Protease from Abrus precatorius Linn. (Fabaceae) from Cameroon. Adv. enzym res. 2016; 4(2):35-43.
Chiwook P. Visual Interpretation of the Meaning of kcat/KM in Enzyme Kinetics. J. Chem. Educ. 2022; 99 (7):2556–2562.
Mulry KR, Hanson BA, Dudle DA. Alternative Strategies in Response to Saline Stress in Two Varieties of Portulaca oleracea (Purslane). PloS one. 2015;10(9): e0138723.
Kim HR, Tagirasa R, Yoo E. Covalent Small Molecule Immunomodulators Targeting the Protease Active Site. J. Med. Chem.2021: 64(9):5291-5322.
Allahmoradi E, Taghiloo S, Omrani-Nava V, Shobeiri SS, Tehrani M, Ebrahimzadeh MA, Asgarian-Omran H. Antiinflammatory effects of the Portulaca oleracea hydroalcholic extract on human peripheral blood mononuclear cells. Med J Islam Repub Iran. 2018; 3:32-80.
Sarveswaran R, Jayasuriya W, Suresh T. In vitro assays to investigate the anti-inflammatory activity of herbal extracts a review. World J. Pharm. Res.2017; 6(17):131-141.
Saleem A, Saleem M, Akhtar MF. Antioxidant, antiinflammatory and antiarthritic potential of Moringa oleifera Lam: An ethnomedicinal plant of Moringaceae family. S. Afr. J. Bot.2020; 128:246-256.
Gai M, Federico T, Cristiana V, Fiammetta V, Silvia B, Selina P, Gaia E, Francesco S, Alessandra M, Laura A, Anna S, Anna B, Nicoletta L, Jacqueline B, Wieland B. ASPM and CITK regulate spindle orientation by affecting the dynamics of astral microtubules. EMBO Rep .2016; 17(10):1396-1409.
Capuana M. A review of the performance of woody and herbaceous ornamental plants for phytoremediation in urban areas. IFOREST. 2020; 13(2):139.-151
Awosika T, Aluko RE. Enzymatic pea protein hydrolysates are active trypsin and chymotrypsin inhibitors. Foods, 2019; 8(6): 200.- 206
Abhijeet PH, Sainath SK, Niraj R, Shadab A, Jaswinder S, Pankaj K. Characterization of a Bowman–Birk type trypsin inhibitor purified from seeds of Solanum surattense. Sci. Rep. 2021; 11: 8648.
Singh A, Benjakul S. Proteolysis and its control using protease inhibitors in fish and fish products: A review. Compr. Rev. Food Sci. Food Saf.2018; 17(2): 496-509.
Singh A, Benjakul S. Serine protease inhibitors from squid ovary: Extraction and its effect on proteolysis and gel properties of surimi. J. Food Sci. Technol.2017; 54(1): 267-275.
Malomo S A, Aluko RE. In vitro acetylcholinesteraseinhibitory properties of enzymatic hemp seed protein hydrolysates. J. Am. Oil Chem. Soc.2016: 93(3): 411-420.
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