The Effect of Red Dragon Fruit (Hylocereus polyrhizus) Peel Ethanol Extract on Oxidative Stress in Sprague Dawley Rats (Rattus norvegicus)
doi.org/10.26538/tjnpr/v6i6.7
Keywords:
Fruit peel extract, Heatstress, Malondialdehyde, Oxidative stress, Red dragonAbstract
Heat stress can induce DNA, lipid, and protein damage by releasing inflammatory mediators and metabolic reactive oxygen species. The antiradical activity of red dragon fruit (Hylocereus polyrhizus) has been reported in several studies, but there is little information on the effect of its extract on heat stress exposure. Therefore, this study was conducted to investigate the effects of red dragon peel ethanol extract on acute heat stress in Sprague Dawley rats (Rattus norvegicus). Ethanol extract was prepared from red dragon fruits. Thirty rats were divided into five groups and treated for 14 days. Rats were fed a standard diet in the negative and positive control groups (K1 and K2, respectively). Furthermore, treatment groups P1, P2, and P3 received dragon fruit peel ethanol extract on the 14th day at doses of 400, 800, or 1,600 mg/kg BW, respectively. On the 15th day, heat exposure was used to create stress in K2, P1, P2, and P3. The oxidation state was determined by analyzing malondialdehyde (MDA) levels. The results of the MDA levels in rats in the K1, K2, P1, P2, and P3 groups were 19.50 ± 0.85, 49.30 ± 3.70, 20.65 ± 4.19, 20.37 ± 2.63, and 22.44 ± 6.68 ng/mL, respectively. Furthermore, significant (p < 0.001) values were observed in the K2 group, as well as nominal differences between the treatment groups P1, P2, and P3 (p > 0.05). Based on these findings, red dragon fruit extract improves an oxidative stress biomarker in acute heat-induced stress rats. However, the impact is not dose-dependent.
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