Green Synthesis, Characterization and Antibacterial Potential of Silver Nanoparticles from Onosma bracteatum Extract doi.org/10.26538/tjnpr/v6i2.6
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Abstract
Antimicrobial resistance is one of the major concerns nowadays. In the present era, green nanotechnology has emerged. Plant materials such as stems, roots, and flowers in the form of an extract have been successfully used in the green synthesis of nanoparticles. The present study was aimed at biologically synthesizing silver nanoparticles (AgNPs) using Onosma bracteatum extract and investigating their antibacterial activity against different human pathogenic bacteria. The hydroalcoholic extract was prepared from the leaves and stems of O. bracteatum. The extract was used to biologically synthesize AgNPs. The green synthesized AgNPs were characterized using UV-Vis spectroscopy, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy techniques. The antibacterial potential of different concentrations (0.5 to 1 mg/mL) of the green synthesized AgNPs was tested against Bacillus subtitis, Micrococcus luteus, Staphylococcus epidermidis, Bacillus pumilus, Staphylococcus aureus, and Escherichia coli. The results of the UV-Vis spectroscopy confirmed the synthesis of AgNPs with an absorption peak in the visible range of 380 - 500 nm. According to the FTIR analysis, the extract and AgNPs contained functional groups, which may have the ability to reduce Ag+ to Ag0 by supplying electrons to silver and therefore stabilizing the AgNPs formed. The AgNPs are crystalline, have face-centered-cubic geometry, and are spherical. Also, the green synthesized AgNPs have a significant dose-dependent bactericidal effect on gram-positive Staphylococcus aureus and gram-negative E. coli. The findings of the study suggest that plant extract could be used to synthesize metal nanoparticles, with the green nanoparticles having biomedical applications.
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