Viability of α-mangostin and Lipopolysaccharide (LPS) in 7F2 Cells
http://www.doi.org/10.26538/tjnpr/v7i1.22
Keywords:
medicine, socket preservation, MTT assay, Lipopolysaccharide, α-mangostinAbstract
Based on data from RISKESDAS in 2018, the incidence of tooth extraction in Indonesia is 19%. In post-extraction of teeth, the gingival tissue around the extraction area is inflamed as well as a decrease in the dimensions of the alveolar ridge. Socket preservation is performed to reduce the possibility of bone resorption and bone formation. Alpha(α)-mangostin from mangosteen peel extract (Garcinia mangostana Linn.) is expected to accelerate post-extraction healing so that implant placement can be more adequate. The study analyzed the viability of α-mangostin and lipopolysaccharide on 7F2 cells lines. Microtetrazolium (MTT) assay against 7F2 cell culture was used. The correlation between concentration and viability of Lipopolysaccharide (LPS) and α- mangostin was interpreted by statistical analysis. The viability of the 7F2 cell culture increased in line with the increase in the concentration of α-mangostin and the optimal concentration of 8.25 g/mL with the highest percentage of living cells at 132.120%. α-mangostin can increase the viability of 7F2 cell culture and it has the potential to be used as an alternative material to reduce inflammation after tooth extraction.
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