<i>In Vivo</i> Assessment of Nanocapsules of Black Rice (<i>Zizania aquatica</i>) Yeast Extract in Diabetes Mellitus Type 2-Induced Mice (<i>Mus musculus</i>)
http://www.doi.org/10.26538/tjnpr/v7i1.23
Keywords:
Type 2 diabetes mellitus, Poloxamer 88, Insulin resistance, Glucose tolerance, Antiglycemic effectAbstract
Nano-products are commonly used in pharmaceuticals. This study investigated the in vivo antiglycemic effect of nanocapsules of black rice (Zizania aquatica) yeast aquadest extract in mice (Mus musculus) induced with type 2 diabetes mellitus (T2DM). Black rice yeast was prepared and extracted with distilled water. The black rice yeast extract was used to producenanocapsules using a poloxamer 88 support matrix. The in vivo antiglycemic effect of black rice yeast extract (BRY-AE) nanocapsules was investigated using T2DM-induced male Swiss mice. The mice were divided into 4 groups, including the positive control group (DM + commercial feed), the negative control group (no DM + commercial feed), the BRY-AE nanocapsule test group (DM + BRY-AE nanocapsules+feed), and the extract test group (DM + BRY AE+commercial feed). Changes in glucose levels, profiles of glucose tolerance, profiles of insulin resistance, and histological observations of liver tissue were determined. The results showed that blood glucose levels in all groups reduced after the second week, however, some still did not reach normal levels (no DM). The glucose tolerance test for the nano-treatment of BRY-AE at 800 ppm and BRY-AE at 1000 ppm revealed a good profile, indicating that it was comparable to the negative control and metformin. When compared to the negative control, the insulin sensitivity of the nanocapsules from BRY-AE at 800 ppm was relatively low. The findings of this study suggest that the nanocapsules BRY-AE can be used to lower blood glucose levels in mice with T2DM.
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