Momordica charantia Fruit Extract on Cardiac Biomarker Serum Attenuation in Rats and its Bioactive Compound Molecular Docking Against SIRT-1 Protein
http://www.doi.org/10.26538/tjnpr/v7i1.21
Keywords:
SIRT1, CKMB, LDH, Momordica charantia, Molecular dockingAbstract
Momordica charantia has various biological activities as antihyperglycemic, anti-inflammatory, and antioxidant. Thisstudy evaluated Momordica charantia fruit extract against the LDH and CKMB serum in rats induced with isoproterenol. We also evaluated the molecular docking of bioactive compounds in Momordica charantia fruit against the SIRT1 protein. Wistar male rats were divided into four groups: negative control (Na-CMC), positive control (Na-CMC and isoproterenol), and resveratrol group at 9 mg/kg b.wt, and Momordica charantia fruit extract at 300 mg/kg b.w with isoproterenol. The interaction between ligand and receptor was evaluated and calculated with molecular docking software. The ligands were docked using the AutoDock Tools - 1.5.7. The binding affinity was calculated using the binding free energy (kcal/mol). The results showed that Momordica charantia fruit extract at 300 mg/kg b.w could attenuate the LDH (49.37%) and CKMB (44.5%) increase in serum after isoproterenol-induced myocardial infarction in rats. Molecular docking of selected compounds from Momordica charantia fruit extract presents that momordicilin has great potential as a SIRT-1 activator with a binding energy of - 11.3 kcal/mol. These findings implicate that Momordica charantia fruit extract can be developed further as a potential cardioprotective drug.
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