Antifungal Effect of Rosmarinus officinalis L. and Salvia officinalis L. Extracts against Tomato Grey Mould doi.org/10.26538/tjnpr/v6i2.3
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Abstract
Grey mould is a disease caused by Botrytis cinerea. This disease is often controlled by fungicides which pose a real threat to human health and the environment. This study is part of the search for harmless and non-polluting alternatives, such as aromatic and medicinal plant extracts. Thus, organic extracts of Rosmarinus officinalis L. and Salvia officinalis L. were tested for their antifungal properties against Botrytis cinerea in vitro (on PDA medium), on mycelial growth and in vivo, on tomato plant and fruit rot. The results obtained revealed that in vitro, the extracts of R. officinalis L. and S. officinalis L. all exceeded 50% mycelial growth inhibition at 800 ppm. By comparing the IC50 values, petroleum ether extract of Salvia officinalis L. is the most effective (IC50 = 161.65 ppm), while the methanol extracts of the two plants studied are the least effective. In vivo only petroleum ether and chloroform extracts exceed 50% of rot inhibition on plant and fruit in both of preventive treatment and curative treatment. While methanol extracts do not exceed 20% and 50% of rot inhibition on plants and fruit respectively. The thin Layer chromatography (TLC) analyses revealed that the extracts studied are rich in terpenes, sterols and polyphenols, such as tannins and coumarins.
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