Inhibition of D – Amino Acid Oxidase by Chromatographic Fractions and Kaempferol-3-O-rutinoside Isolated from Philenoptera cyanescens Leaves http://www.doi.org/10.26538/tjnpr/v7i1.25
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Abstract
Philenoptera cyanescens (PC) leaves are used ethno-medically for the treatment of mental disorder in Southwestern Nigeria. The crude extract and solvent fractions of the PC leaves have earlier been reported for in vivo antipsychotic properties in rodents. The D-amino acid oxidase (DAO) assay is an in vitro anti-psychotic model. However, there is little or no report on the inhibition of DAO by medicinal plants. This study investigates the in vitro antipsychotic activity of a bioactive compound, DCM and EtOAc chromatographic sub-fractions from Philenoptera cyanescens leaves. The inhibitory effect of the isolated compound, sub-fractions from DCM (a-j) and EtOAc (A-K), Risperidone and Haloperidol standards were tested on Pig Kidney D-amino acid oxidase enzyme (pkDAO), using D–Kynurenine as substrate. Percentage inhibition was measured in a fluorescence microplate reader with an excitation and emission wavelengths of 355 and 460 nm, respectively. Structure of isolated compound was elucidated using NMR and FT-IR analyses. PCDe (61.9%) at 0.2 mg/mL showed a higher percentage inhibition than Risperidone (43.2%) and Haloperidol (18.3%) among the PC DCM sub-fractions. Interestingly, PCEF gave 100% inhibition of the enzyme, when compared with other sub-fractions and standards. The ethyl acetate sub-fraction F showed best inhibitory activity, Kaempferol-3-O-rutinoside showed percentage inhibition of 13.7% comparable to the standard drug, Haloperidol. This compound has been reported earlier but isolated from this plant and tested on the antipsychotic property using D amino acid oxidase for the first time.
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