Biological Activities of Extracts and Secondary Metabolites from Millettia phuwuaensis http://www.doi.org/10.26538/tjnpr/v7i1.17

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Published: Feb 1, 2023
Keywords:
Millettia phuwuaensis, Fabaceae, Isoflavonoids, Rotenoids, Biological activity

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Penpaka Chaikrueang
Department of Chemistry, Faculty of Science and Center of Innovation in Chemistry, Lampang Rajabhat University,Lampang, Thailand
Wilart Pompimon
Department of Chemistry, Faculty of Science and Center of Innovation in Chemistry, Lampang Rajabhat University,Lampang, Thailand
Phansuang Udomputtimekakul
Department of Chemistry, Faculty of Science and Center of Innovation in Chemistry, Lampang Rajabhat University,Lampang, Thailand
Nopawit Khamto
Faculty of Science, Chiang Mai University, Chiang Mai, Thailand
Puttinan Meepowpan
Faculty of Science, Chiang Mai University, Chiang Mai, Thailand
Pakpoom Natetip
Department of Chemistry, Faculty of Science and Center of Innovation in Chemistry, Lampang Rajabhat University,Lampang, Thailand
Nuntiya Khudngaongam
Department of Chemistry, Faculty of Science and Center of Innovation in Chemistry, Lampang Rajabhat University,Lampang, Thailand
Napakaon Wongjaren
Department of Chemistry, Faculty of Science and Center of Innovation in Chemistry, Lampang Rajabhat University,Lampang, Thailand
Duangsuda Khuntee
Department of Chemistry, Faculty of Science and Center of Innovation in Chemistry, Lampang Rajabhat University,Lampang, Thailand
Khwanruethai Michaidi
Department of Chemistry, Faculty of Science and Center of Innovation in Chemistry, Lampang Rajabhat University,Lampang, Thailand
Khanittha Kongbun
Department of Chemistry, Faculty of Science and Center of Innovation in Chemistry, Lampang Rajabhat University,Lampang, Thailand
Supaporn Chueakhamsao
Kanyawee Issariyajongkol
Department of Chemistry, Faculty of Science and Center of Innovation in Chemistry, Lampang Rajabhat University,Lampang, Thailand
Narong Nuntasaen
The Forest Herbarium, Department of National Park, Wildlife and Plant Conservation, Ministry of Natural Resources and Environment, Bangkok, Thailand
Kanoknetr Suksen
Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand
Arthit Chairoungdua
Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand
Jitra Limthongkul
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand
Chanita Naparswad
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand
Nichapa Charoenphakinrattana
Life group international company limited, 78/2, Village No. 5, Khao Changum, Photharam, Ratchaburi, Thailand
Suttiporn Pikulthong
Department of Chemistry, Faculty of Science, Mahidol University, Bangkok, Thailand

Abstract

A study of the phytochemicals constituents of the stems of, Millettia phuwuaensis has led to the isolation of 7-methoxy-5/,6/- methylenedioxyisoflavone (1), and 12-deoxo-12α-hydroxyelliptone (2. Their  structures were confirmed using NMR spectroscopy. Both extracts and the  purified compounds were evaluated for their antibacterial, anti-HIV, and cancer  activity. The antibacterial test results of the extracts and pure compound were  found to be valuable, MIC is in the range of 12.5-200 mg/mL, in the range of  0.188-6 mg/mL, respectively. Mechanistic anti-HIV affect RT and MC99 found  that ethyl acetate extract inhibited the very high level with IC50 with 75.93%. It was also found that all extracts were effective in inhibiting AIDs by mechanism MC99 at EC50 at 1.35 µM (TI>2.41). Further, the ethyl acetate extract showed  marked cytotoxicity (ED50 = 17.58 µg/ml against the SH-SY5Y cancer cell line.  Additionally, compound 1 also exhibited RT, moderately active with IC50 55.19 %  inhibition. More than that, compounds 1 and 2 also exhibited MC99 at 50%  (EC50) values of > 3.01 (TI >1.70) and 1.78 (TI >1.70), respectively.

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How to Cite
Chaikrueang, P., Pompimon, W., Udomputtimekakul, P., Khamto, N., Meepowpan, P., Natetip, P., Khudngaongam, N., Wongjaren, N., Khuntee, D., Michaidi, K., Kongbun, K., Chueakhamsao, S., Issariyajongkol, K., Nuntasaen, N., Suksen, K., Chairoungdua, A., Limthongkul, J., Naparswad, C., Charoenphakinrattana, N., & Pikulthong, S. (2023). Biological Activities of Extracts and Secondary Metabolites from Millettia phuwuaensis: http://www.doi.org/10.26538/tjnpr/v7i1.17. Tropical Journal of Natural Product Research (TJNPR), 7(1), 2207-2212. https://tjnpr.org/index.php/home/article/view/1550
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Vol. 7 No. 1 (2023): Tropical Journal of Natural Product Research (TJNPR)
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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Author Biography

Arthit Chairoungdua, Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand


5Excellent Center for Drug Discovery (ECDD), Mahidol University, Bangkok, Thailand
6Toxicology Graduate Program, Faculty of Science, Mahidol University, Bangkok, Thailand

How to Cite

Chaikrueang, P., Pompimon, W., Udomputtimekakul, P., Khamto, N., Meepowpan, P., Natetip, P., Khudngaongam, N., Wongjaren, N., Khuntee, D., Michaidi, K., Kongbun, K., Chueakhamsao, S., Issariyajongkol, K., Nuntasaen, N., Suksen, K., Chairoungdua, A., Limthongkul, J., Naparswad, C., Charoenphakinrattana, N., & Pikulthong, S. (2023). Biological Activities of Extracts and Secondary Metabolites from Millettia phuwuaensis: http://www.doi.org/10.26538/tjnpr/v7i1.17. Tropical Journal of Natural Product Research (TJNPR), 7(1), 2207-2212. https://tjnpr.org/index.php/home/article/view/1550

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