Evaluation of Antibacterial, Antioxidant and Anti-inflammatory Properties of Methanol Extract of Varthemia iphionoides http://www.doi.org/10.26538/tjnpr/v7i1.4
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Abstract
Medicinal plants represent an essential source of active biological compounds. The current study examined the methanol extract of V. iphionoides for its antibacterial, antioxidant, and antiinflammatory activities. The antibacterial activity was evaluated against six bacterial strains (Staphylococcus xyloses, Escherichia coli, Klebsiella oxytoca, Enterobacter aerogenes, Achromabacter xylosoxidans, and Pseudomonas aerugenosa). V. iphionoides extract showed good antibacterial activity against Staphylococcus xylosus followed by, pseudomonas aeruginosa and Klebsiella oxytoca, and the minimum inhibitory concentrations (MICs) were 33, 33, and 11 µg/ml, respectively. However, the extract did not show any antibacterial activity against the other bacterial strains. The antioxidant activity of the plant extract was evaluated using ABTS and DPPH radical scavenging assays, and the Trolox equivalents were 93±0.31 and 84.8±0.28 mg Trolox/g plant extract, respectively. Anti-inflammatory activity in LPS-induced mice was investigated by measuring the concentration of pro-inflammatory cytokines TNF-alpha, IL 1beta, and antioxidant enzyme catalase and glutathione peroxidase. The results demonstrated that the highest concentration of V. iphionoides extract was the most efficient. Anti-inflammatory activity was exhibited by reducing the concentrations of pro-inflammatory TNF-α and IL 1 beta and reducing the concentrations of antioxidant enzyme catalase and glutathione peroxidase compared to the negative control group.
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Muthee JK, Gakuya DW, Mbaria JM, Kareru PG, Mulei CM Njonge FK. Ethnobotanical study of anthelmintic and other medicinal plants traditionally used in Loitoktok district of Kenya. J Ethnopharmacol. 2011; 135(1):15-21.
Pereira RF, Bartolo PJ. Traditional therapies for skin wound healing. Adv wound care. 2016;5(5):208-229.
Ferri M, Ranucci E, Romagnoli P, Giaccone V. Antimicrobial resistance: A global emerging threat to public health systems. Crit Rev Food Sci Nutr. 2017; 57(13):2857- 2876.
Khleifat KM. Biodegradation of phenol by Actinobacillus sp.: Mathematical interpretation and effect of some growth conditions. Bioremediat J. 2007; 11(3):103-112.
Alsarayreh AZ, AttalahOran S, Mutie Shakhanbeh J,. In vitro and in vivo wound healing activities of Globularia arabica leaf methanolic extract in diabetic rats. J Cosmet Dermatol. 2021; 9:13-23.
Chang H-Y, Sheu M-J, Yang C-H, . Analgesic effects and the mechanisms of anti-inflammation of hispolon in mice. EvidenceBased Complement Altern Med. 2011;2011.
Alsarayreh AZ, Oran SA, Shakhanbeh JM. Evaluation of antiinflammatory activity of methanol extract of Rhus Coriaria L. In diabetic rats. Trop J Nat Prod Res. 2021; 5(8):1409-1413.
Al Qaisi YT, Khleifat KM, Alfarrayeh II, Alsarayreh AZ. In Vivo Therapeutic Effect of Some Medicinal Plants’ Methanolic Extracts on the Growth and Development of Secondary Hydatid Cyst Infection. Acta Parasitol. Published online 2022:1-14.
Alfarrayeh I, Aloshoush A, Ahmad A, Al Qaisi Y. Survey of medicinal plants sold in local markets in Ghor As-Safi, Southern Jordan. Med Plants-International J Phytomedicines Relat Ind. 2022;14(2):340-343.
Al-Soub A, Khleifat K, Al-Tarawneh A, . Silver nanoparticles biosynthesis using an airborne fungal isolate, Aspergillus flavus: optimization, characterization and antibacterial activity. Iran J Microbiol. 2022;14(4):518-528.
Khleifat KM, Matar SA, Jaafreh M, Qaralleh H, Al-limoun M O, Alsharafa KY. Essential Oil of Centaurea damascena Aerial Parts, Antibacterial and Synergistic Effect. J. of Essential Oil Bearing Plants. 2019; 22(2):356-367.
Al-Tawarah NM, Qaralleh H, Khlaifat AM,. Anticancer and Antibacterial Properties of Verthemia Iphionides Essential Oil/Silver Nanoparticles. Biomed Pharmacol J. 2020; 13(3):1175-1184.
Khleifat K, Alqaraleh M, Al-limoun M,. The ability of rhizopus stolonifer MR11 to biosynthesize silver nanoparticles in response to various culture media components and optimization of process parameters required at each stage of biosynthesis. J Ecol Eng. 2022; 23(8):89-100.
Alsarayreh AZ, Oran SA, Shakhanbeh JM,. Efficacy of methanolic extracts of some medicinal plants on wound healing in diabetic rats. Heliyon. Published online 2022:e10071.
Gorelick J, Kitron A, Pen S, Rosenzweig T, Madar Z. Antidiabetic activity of Chiliadenus iphionoides. J Ethnopharmacol. 2011; 137(3):1245-1249.
Kasabri V, Abu-Dahab R, Afifi FU, Naffa R, Majdalawi L, Shawash H. In vitro effects of Geranium graveolens, Sarcopoterium spinosum and Varthemia iphionoides extracts on pancreatic MIN6 proliferation and insulin secretion and on extrapancreatic glucose diffusion. Int J Diabetes Dev Ctries. 2013; 33(3):170-177.
Budovsky A, Shteinberg A, Maor H,. Uncovering the geroprotective potential of medicinal plants from the Judea region of Israel. Rejuvenation Res. 2014; 17(2):134-139.
Khleifat KM, Abboud MM, Al-Mustafa AH. Effect of Vitreoscilla hemoglobin gene (vgb) and metabolic inhibitors on cadmium uptake by the heterologous host Enterobacter aerogenes. Process Biochem. 2006; 41(4):930-934.
Alsarayreh AZ, Oran SA, Shakhanbeh JM. Effect of Rhus coriaria L. methanolic fruit extract on wound healing in diabetic and non‐diabetic rats. J Cosmet Dermatol. Published online 2021.
Alzoreky NS, Nakahara K. Antibacterial activity of extracts from some edible plants commonly consumed in Asia. Int J Food Microbiol. 2003; 80(3):223-230.
Khleifat KM, Tarawneh KA, Ali Wedyan M, Al-Tarawneh AA, Al Sharafa K. Growth kinetics and toxicity of Enterobacter cloacae grown on linear alkylbenzene sulfonate as sole carbon source. Curr Microbiol. 2008; 57(4):364-370.
Khleifat KM, Sharaf EF, Al-limoun MO. Biodegradation of 2-chlorobenzoic acid by Enterobacter cloacae: Growth kinetics and effect of growth conditions. Bioremediat J. 2015; 19(3):207-217.
Qaralleh H, Khleifat KM, Al-Limoun MO, Alzedaneen FY, Al-Tawarah N. Antibacterial and synergistic effect of biosynthesized silver nanoparticles using the fungi Tritirachium oryzae W5H with essential oil of Centaurea damascena to enhance conventional antibiotics activity. Adv Nat Sci Nanosci Nanotechnol. 2019;10(2):25016.
Gabriel-Ajobiewe R, Ifesan BOT, Ayoko OM. Comparison of the antioxidant and phytochemical properties of dawadawa (sub-sahara Africa condiment) from Mucuna pruriens L. and Parkia biglobosa Jacg. under control condition. J Microbiol Biotechnol Food Sci. 2021; 2021:705- 710.
Wu Y, Ding Y, Tanaka Y, Zhang W. Risk factors contributing to type 2 diabetes and recent advances in the treatment and prevention. Int J Med Sci. 2014; 11(11):1185.
Ramakrishnan MA, Muthuchelvan D. Influence of ReedMuench median dose calculation method in virology in the millennium. Nature. 2018; 8:9633.
Chograni H, Riahi L, Zaouali Y, Boussaid M. Polyphenols, flavonoids, antioxidant activity in leaves and flowers of T unisian G lobularia alypum L.(G lobulariaceae). Afr J Ecol. 2013; 51(2):343-347.
Dellavalle PD, Cabrera A, Alem D, Larrañaga P, Ferreira F, Dalla Rizza M. Antifungal activity of medicinal plant extracts against phytopathogenic fungus Alternaria spp. Chil J Agric Res. 2011; 71(2):231-241.
Al Qaisi YT, Khleifat KM, Oran SA,. Ruta graveolens, Peganum harmala, and Citrullus colocynthis methanolic extracts have in vitro protoscolocidal effects and act against bacteria isolated from echinococcal hydatid cyst fluid. Arch Microbiol. 2022; 204(4):1-13.
Alsarayreh AZ, AttalahOran S, Mutie Shakhanbeh J. In vitro and in vivo wound healing activities of Globularia arabica leaf methanolic extract in diabetic rats. J Cosmet Dermatol. https://doi.org/10.1093/jbcr/irac089
Angioni A, Barra A, Coroneo V, Dessi S, Cabras P. Chemical composition, seasonal variability, and antifungal activity of Lavandula stoechas L. ssp. stoechas essential oils from stem/leaves and flowers. J Agric Food Chem. 2006; 54(12):4364-4370.
Masadeh MM, Alkofahi AS, Tumah HN, Mhaidat NM, Alzoubi KH. Antibacterial activity of some medicinal plants grown in Jordan. Pak J Pharm Sci. 2013; 26(2):267-271.
Afifi FÜ, Al‐Khalil S, Abdul‐Haq BK,. Antifungal flavonoids from Varthemia iphionoides. Phyther Res. 1991; 5(4):173-175.
Abu-Romman SM, Haddad MA, Al-Hadid KJ. The potential allelopathic effects of Varthemia iphionoides and the identification of phenolic allelochemicals. Jordan J Biol Sci. 2015; 147(3388):1-6.
Gupta VK, Verma S, Gupta S,. Membrane-damaging potential of natural L-(−)-usnic acid in Staphylococcus aureus. Eur J Clin Microbiol Infect Dis. 2012; 31(12):3375- 3383.
Tsuchiya H, Iinuma M. Reduction of membrane fluidity by antibacterial sophoraflavanone G isolated from Sophora exigua. Phytomedicine. 2000;7 (2):161-165.
Al-Dabbas MM, Suganuma T, Kitahara K, Hou D-X, Fujii M. Cytotoxic, antioxidant and antibacterial activities of Varthemia iphionoides Boiss. extracts. J Ethnopharmacol. 2006; 108(2):287-293.
Solórzano-Santos F, Miranda-Novales MG. Essential oils from aromatic herbs as antimicrobial agents. Curr Opin Biotechnol. 2012; 23(2):136-141.
Weydert CJ, Cullen JJ. Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue. Nat Protoc. 2010; 5(1):51-66.
Liao J-C, Deng J-S, Chiu C-S,. Anti-inflammatory activities of Cinnamomum cassia constituents in vitro and in vivo. Evidence-Based Complement Altern Med. 2012;2012.
Beagley J, Guariguata L, Weil C, Motala AA. Global estimates of undiagnosed diabetes in adults. Diabetes Res Clin Pract. 2014; 103(2):150-160.
Fernández-Ortuño D, Pérez-García A, López-Ruiz F, Romero D, De Vicente A, Torés JA. Occurrence and distribution of resistance to QoI fungicides in populations of Podosphaera fusca in south central Spain. Eur J Plant Pathol. 2006; 115(2):215-222.
Mallinckrodt CH, Prakash A, Houston JP, Swindle R, Detke MJ, Fava M. Differential antidepressant symptom efficacy: placebo-controlled comparisons of duloxetine and SSRIs (fluoxetine, paroxetine, escitalopram). Neuropsychobiology. 2007; 56(2-3):73-85.
Subedi L, Cho K, Park YU, Choi HJ, Kim SY. Sulforaphaneenriched broccoli sprouts pretreated by pulsed electric fields reduces neuroinflammation and ameliorates scopolamineinduced amnesia in mouse brain through its antioxidant ability via Nrf2-HO-1 activation. Oxid Med Cell Longev. 2019;2019.
Cai Y, Luo Q, Sun M, Corke H. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci. 2004; 74(17):2157-2184.
Khleifat K, Abboud M, Al-Shamayleh W, Jiries A, Tarawneh K. Effect of chlorination treatment on gram negative bacterial composition of recycled wastewater. Pak J Biol Sci. 2006; 9:1660-1668.
Abdelhalim AR, Al-Munawarah AM. Pharmacological Properties and Chemical Constituents of Chiliadenus iphionoides (Syn. Varthemia iphionoides): A Review. Eur J Med Plants. 2020; 31:84-97.
Gasparetto JC, Martins CAF, Hayashi SS, Otuky MF, Pontarolo R. Ethnobotanical and scientific aspects of Malva sylvestris L.: a millennial herbal medicine. J Pharm Pharmacol. 2012; 64(2):172-189.
Cattaneo A, Cattane N, Galluzzi S,. Association of brain amyloidosis with pro-inflammatory gut bacterial taxa and peripheral inflammation markers in cognitively impaired elderly. Neurobiol Aging. 2017; 49:60-68.
Lanzillo R, Moccia M, Russo CV,. Therapeutic lag in reducing disability progression in relapsing-remitting multiple sclerosis: 8-year follow-up of two randomized addon trials with atorvastatin. Mult Scler Relat Disord. 2019; 28:193-196.
Horowitz RI, Freeman PR. Precision medicine: The role of the MSIDS model in defining, diagnosing, and treating chronic Lyme disease/post treatment Lyme disease syndrome and other chronic illness: Part 2. In: Healthcare. Vol 6. Multidisciplinary Digital Publishing Institute; 2018:129.
Justino PFC, Franco AX, Pontier-Bres R,. Modulation of 5- fluorouracil activation of toll-like/MyD88/NF-κB/MAPK pathway by Saccharomyces oulardii CNCM I-745 probiotic. Cytokine. 2020; 125:154791.
Sorice A, Guerriero E, Capone F, Colonna G, Castello G, Costantini S. Ascorbic acid: its role in immune system and chronic inflammation diseases. Mini Rev Med Chem. 2014; 14(5):444-452.
Huang C, Becker MF, Keto JW, Kovar D. Annealing of nanostructured silver films produced by supersonic deposition of nanoparticles. J Appl Phys. 2007; 102(5):54308.
Yoshinari O, Takenake A, Igarashi K. Trigonelline ameliorates oxidative stress in type 2 diabetic Goto-Kakizaki rats. J Med Food. 2013; 16(1):34-41.
Yeole NB, Sandhya P, Chaudhari PS, Bhujbal PS. Evaluation of Malva sylvestris and Pedalium murex mucilage as suspending agent. Int J PharmTech Res. 2010; 2(1):385-389.
Dehimat A, Azizi I, Baraggan-Montero V, Khettal B. In vitro antioxidant and inhibitory potential of leaf extracts of Varthemia sericea against key enzymes linked to type 2 diabetes. Jordan J Biol Sci. 2021;14(1).
Crascì L, Lauro MR, Puglisi G, Panico A. Natural antioxidant polyphenols on inflammation management: Antiglycation activity vs metalloproteinases inhibition. Crit Rev Food Sci Nutr. 2018; 58(6):893-904.
Shen J, Griffiths PT, Campbell SJ, Utinger B, Kalberer M, Paulson SE. Ascorbate oxidation by iron, copper and reactive oxygen species: review, model development, and derivation of key rate constants. Sci Rep. 2021; 11(1):1-14