Combination of Hydration and Ascorbic Acid Synergistically Prevents Organophosphate Pesticide Toxicity of Vital Organs in Experimental Rat Model

doi.org/10.26538/tjnpr/v6i8.23

Authors

  • Ikenna K. Uchendu Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Ozioma E. Obianyido Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Kelvin N. Orji Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Amarachi C. Uba Department of Biomedical Technology, University of Port Hacourt, Rivers State, Nigeria
  • Juliet U. Joboson Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Cyprian E. Oguji Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Ebubechukwu L. Ofojama Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Ukam E. Etim Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Blessing C. Oguzie Department of Medical Laboratory Science, Abia State University, Uturu, Abia State, Nigeria
  • Ifunanyachukwu C. Onuchukwu Department of Medical Laboratory Science, Abia State University, Uturu, Abia State, Nigeria
  • Chibuike E. Maduekwe Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Chikwube C. Ajibo Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Favour C. Sunday Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Comfort C. Igwe Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Chimdike G. Omejua Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Chibueze J. Obigeorge Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Chisom N. Okafor Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Victor C. Makata Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Sharon O. Eze Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Onyinyechi V. Ifionu Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Chisimdindu F. Nnaji Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Vivian O. Nnanyereugo Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Carmilla I. Udensi Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Hilary Emuebie Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Ejiofor M. Agbo Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Unoaku J. Ikwueze Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
  • Olayinka T. Amokeoja Department of Health Information Management, Obafemi Awolowo University Teaching Hospital, Ife, Osun State, Nigeria
  • Chiagoziem H. Ekemezie Department of Medical Laboratory Science, Nnamdi Azikikwe University, Awka, Anambra State, Nigeria
  • Sheriffdeen A. Adebisi Department of Pharmacy, Olabisi Onabanjo University. Ogun State, Nigeria
  • Prince I. Onuoha Department of Medical Laboratory Science, University of Lagos, Lagos State, Nigeria

Keywords:

Organ toxicity,, Organophosphate pesticides,, Vitamin C,, Water hydration

Abstract

Triazophos, an organophosphate acetylcholinesterase inhibitor and synthetic organic thiophosphate molecule, is employed as a pesticide. It is a yellowish-brown oily liquid, and exposure can happen through inhalation, ingestion, or touch. This study evaluated the toxic effects of organophosphate pesticide exposure on several vital organs, and the therapeutic potential of ascorbic acid (vitamin C) or water hydration alone or their combination. Random grouping of 25 adult male Wistar rats into five (A-E) was done. A-D Groups received pesticide (0.5mL/kg, i.p) for 3 days. B and D groups were treated with Vitamin C (200mg/kg, oral) only. Groups C and D received oral hydration with distilled water only (15mL/kg) for 3 days. Only pesticide was given to group A (negative control); while group E received no administration. The functions of the kidneys, liver, testicles and heart respectively were determined by measuring serum levels of urea, creatinine, K+and Na+; Alanine transaminase (ALT), Aspatate transaminase (AST), Alkaline phosphatase (ALP) And total bilirubin; testosterone; and lipid profiles (Total cholesterol (TC), Triglycerides (TG), and Low density lipoprotein cholesterol (LDL-C) and by histopathological examination of the kidneys, liver, testes and heart using standard methods. The pesticide exposure alone caused a marked toxicity to the vital organs. Vitamin C alone showed significant ameliorative effect on the organs (p<0.05). Distilled water alone indicated a non-significant difference improvement (p>0.05). Interestingly, there was very marked restoration after coadministration of vitamin C and distilled water (p<0.05 or p<0.01). The findings of histopathology matched the biochemical findings. The combination of water hydration and ascorbic acid synergistically protected these vital organs against toxic chemicals.

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Published

2022-08-01

How to Cite

K. Uchendu, I., E. Obianyido, O., N. Orji, K., C. Uba, A., U. Joboson, J., E. Oguji, C., … I. Onuoha, P. (2022). Combination of Hydration and Ascorbic Acid Synergistically Prevents Organophosphate Pesticide Toxicity of Vital Organs in Experimental Rat Model: doi.org/10.26538/tjnpr/v6i8.23. Tropical Journal of Natural Product Research (TJNPR), 6(8), 1297–1304. Retrieved from https://tjnpr.org/index.php/home/article/view/1448

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