Combination of Hydration and Ascorbic Acid Synergistically Prevents Organophosphate Pesticide Toxicity of Vital Organs in Experimental Rat Model doi.org/10.26538/tjnpr/v6i8.23

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Published: Aug 1, 2022
Keywords:
Organ toxicity,, Organophosphate pesticides,, Vitamin C,, Water hydration

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Ikenna K. Uchendu
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Ozioma E. Obianyido
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Kelvin N. Orji
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Amarachi C. Uba
Department of Biomedical Technology, University of Port Hacourt, Rivers State, Nigeria
Juliet U. Joboson
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Cyprian E. Oguji
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Ebubechukwu L. Ofojama
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Ukam E. Etim
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Blessing C. Oguzie
Department of Medical Laboratory Science, Abia State University, Uturu, Abia State, Nigeria
Ifunanyachukwu C. Onuchukwu
Department of Medical Laboratory Science, Abia State University, Uturu, Abia State, Nigeria
Chibuike E. Maduekwe
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Chikwube C. Ajibo
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Favour C. Sunday
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Comfort C. Igwe
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Chimdike G. Omejua
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Chibueze J. Obigeorge
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Chisom N. Okafor
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Victor C. Makata
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Sharon O. Eze
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Onyinyechi V. Ifionu
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Chisimdindu F. Nnaji
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Vivian O. Nnanyereugo
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Carmilla I. Udensi
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Hilary Emuebie
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Ejiofor M. Agbo
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Unoaku J. Ikwueze
Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
Olayinka T. Amokeoja
Department of Health Information Management, Obafemi Awolowo University Teaching Hospital, Ife, Osun State, Nigeria
Chiagoziem H. Ekemezie
Department of Medical Laboratory Science, Nnamdi Azikikwe University, Awka, Anambra State, Nigeria
Sheriffdeen A. Adebisi
Department of Pharmacy, Olabisi Onabanjo University. Ogun State, Nigeria
Prince I. Onuoha
Department of Medical Laboratory Science, University of Lagos, Lagos State, Nigeria

Abstract

Triazophos, an organophosphate acetylcholinesterase inhibitor and synthetic organic thiophosphate molecule, is employed as a pesticide. It is a yellowish-brown oily liquid, and exposure can happen through inhalation, ingestion, or touch. This study evaluated the toxic effects of organophosphate pesticide exposure on several vital organs, and the therapeutic potential of ascorbic acid (vitamin C) or water hydration alone or their combination. Random grouping of 25 adult male Wistar rats into five (A-E) was done. A-D Groups received pesticide (0.5mL/kg, i.p) for 3 days. B and D groups were treated with Vitamin C (200mg/kg, oral) only. Groups C and D received oral hydration with distilled water only (15mL/kg) for 3 days. Only pesticide was given to group A (negative control); while group E received no administration. The functions of the kidneys, liver, testicles and heart respectively were determined by measuring serum levels of urea, creatinine, K+and Na+; Alanine transaminase (ALT), Aspatate transaminase (AST), Alkaline phosphatase (ALP) And total bilirubin; testosterone; and lipid profiles (Total cholesterol (TC), Triglycerides (TG), and Low density lipoprotein cholesterol (LDL-C) and by histopathological examination of the kidneys, liver, testes and heart using standard methods. The pesticide exposure alone caused a marked toxicity to the vital organs. Vitamin C alone showed significant ameliorative effect on the organs (p<0.05). Distilled water alone indicated a non-significant difference improvement (p>0.05). Interestingly, there was very marked restoration after coadministration of vitamin C and distilled water (p<0.05 or p<0.01). The findings of histopathology matched the biochemical findings. The combination of water hydration and ascorbic acid synergistically protected these vital organs against toxic chemicals.

Article Details

How to Cite
K. Uchendu, I., E. Obianyido, O., N. Orji, K., C. Uba, A., U. Joboson, J., E. Oguji, C., L. Ofojama, E., E. Etim, U., C. Oguzie, B., C. Onuchukwu, I., E. Maduekwe, C., C. Ajibo, C., C. Sunday, F., C. Igwe, C., G. Omejua, C., J. Obigeorge, C., N. Okafor, C., C. Makata, V., O. Eze, S., … I. Onuoha, P. (2022). Combination of Hydration and Ascorbic Acid Synergistically Prevents Organophosphate Pesticide Toxicity of Vital Organs in Experimental Rat Model: doi.org/10.26538/tjnpr/v6i8.23. Tropical Journal of Natural Product Research (TJNPR), 6(8), 1297-1304. https://tjnpr.org/index.php/home/article/view/1448
Issue
Vol. 6 No. 8 (2022): Tropical Journal of Natural Product Research
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Articles
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