In vitro Inhibitory HMG-CoA Reductase Activity of Purified Polyphenol Compounds from Water Lettuce (Pistia Stratiotes) Leaf Extract doi.org/10.26538/tjnpr/v6i7.15
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Abstract
The 3-hydroxy-3-methylglutaryl-coenzyme-A (HMG-CoA) reductase is an enzyme that plays a role in the synthesis of cholesterol. The side effect of synthetic anti-cholesterol drugs has led to the demand for natural HMG-CoA reductase inhibitors such as plant extract. Therefore, this study aimed to determine the inhibitory HMG-CoA reductase activity of polyphenol compounds from water lettuce (Pistia stratiotes) leaf extract. The research was conducted using the crude and purified extracts of water lettuce. The total polyphenols, flavonoids, and HMG-CoA reductase inhibitory activity assay were carried out by in vitro analysis. The values obtained were analyzed quantitatively, followed by the use of an independent sample t-test and presented in graphical form. The results showed that the total polyphenols in each crude and purified extracts were 29.03 mg GAE/g dry sample and 65.63 mg GAE/g dry sample, respectively, while the total flavonoids were 27.58 mg QE/g dry sample and 88.02 mg QE/g dry sample, respectively. The inhibitory activity of the HMG-CoA reductase enzyme showed that the purified extract showed percentage inhibition of 34.74% which was higher than that of the crude extract which was 2.61%. This indicated that the purified extract of water lettuce has higher levels of polyphenols and flavonoids that can inhibit the HMG-CoA reductase enzyme more effectively than the crude extract.
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Bandyopadhyay D, Qureshi A, Ghosh S, Ashish K, Heise LR, Hajra A, Ghosh RK. Safety and Efficacy of Extremely Low LDL-Cholesterol Levels and Its Prospects in Hyperlipidemia Management. J Lipids. 2018; 2018: 1-8.
Nelson RH. Hyperlipidemia as a Risk Factor for Cardiovascular Disease. Prim Care. 2013; 40: 195-211.
Cruz PMR, Mo H, McConathy WJ, Sabnis N, Lacko AG. The Role of Cholesterol Metabolism and Cholesterol Transport in Carcinogenesis: A Review of Scientific Findings, Relevant to Future Cancer Therapeutics. Front Pharmacol. 2013; 4: 119.
Golomb BA and Evans MA. Statin Adverse Effects: A Review of the Literature and Evidence for a Mitochondrial Mechanism. Am J Cardiovasc Drugs. 2008; 8:373-418.
Ramkumar S, Raghunath A, Raghunath S. Statin Therapy: Review of Safety and Potential Side Effects. Acta Cardiol Sin. 2016; 32: 631-639.
Sudirman S, Herpandi, Nopianti R, Lestari SD, Wasahla, Mareta H. Phenolic Contents, Tannin, Vitamin C, and Vitamin E of Water Lettuce (Pistia stratiotes). Orient J Chem. 2017; 33: 3173-3176.
Herpandi, Lestari SD, Bastian, Sudirman S. Antioxidant Activity of the Fractions from Water Lettuce (Pistia stratiotes) Extract. Food Res. 2021; 5: 451-455.
Islam B, Charu C, Adem A, Aburawi E, Ojha S. Insight into The Mechanism of Polyphenols on The Activity of HMGR by Molecular Docking. Drug Des Dev Ther. 2015; 9: 4943- 4951.
Zeka K, Ruparelia K, Arroo R, Budriesi R, Micucci M. Flavonoids and Their Metabolites: Prevention in Cardiovascular Diseases and Diabetes. Dis. 2017, 5.
Dai J and Mumper RJ. Plant Phenolics: Extraction, Analysis and Their Antioxidant and Anticancer Properties. Molecules. 2010; 15: 7313-7352.
Pérez-Magariño S, Ortega-Heras M, Cano-Mozo E. Optimization of a Solid-Phase Extraction Method Using Copolymer Sorbents for Isolation of Phenolic Compounds in Red Wines and Quantification by HPLC. J Agric Food Chem. 2008; 56: 11560-11570.
Chandra S, Khan S, Avula B, Lata H, Yang MH, Elsohly MA, Khan IA. Assessment of Total Phenolic and Flavonoid Content, Antioxidant Properties, and Yield of Aeroponically and Conventionally Grown Leafy Vegetables and Fruit Crops: A Comparative Study. Evid-Based Compl Altern Med. 2014; 2014: 253875.
Barbosa-Pereira L, Pocheville A, Angulo I, Paseiro-Losada P, Cruz JM. Fractionation and Purification of Bioactive Compounds Obtained from a Brewery Waste Stream. Biomed Res Int. 2013; 2013: 1-11.
Yang Z, Tang H, Shao Q, Bilia A, Wang Y, Zhao X. Enrichment and Purification of the Bioactive Flavonoids from Flower of Abelmoschus manihot (L.) Medic using Macroporous Resins. Molecules. 2018; 23: 2649.
Lucci P, Saurina J, Núñez O. Trends in LC-MS and LC- HRMS Analysis and Characterization of Polyphenols in Food. Trends Anal Chem. 2017; 88: 1-24.
Souza JNS, Silva EM, da-Silva MN, Arruda MSP, Larondelle Y, Rogez H. Identification and Antioxidant Activity of Several Flavonoids of Inga edulis Leaves. J Braz Chem Soc. 2007; 18: 1276-1280.
Zhao H, Avena-Bustillos RJ, Wang SC. Extraction, Purification and in vitro Antioxidant Activity Evaluation of Phenolic Compounds in California Olive Pomace. Foods. 2022; 11: 174.
Baskaran G, Shukor MY, Salvamani S, Ahmad SA, Shaharuddin NA, Pattiram PD. HMG-CoA Reductase Inhibitory Activity and Phytocomponent Investigation of Basella alba Leaf Extract as a Treatment for Hypercholesterolemia. Drug Des Dev Ther. 2015; 9: 509– 517.
Seenivasan A. Characterization, Modes of Synthesis, and Pleiotropic Effects of Hypocholesterolemic Compounds - A Review. Enzyme Inhib Med Chem. 2011; 4: 23-32.
Chen ZY, Jiao R, Ma KY. Cholesterol-Lowering Nutraceuticals and Functional Foods, J Agric Food Chem. 2008; 56: 8761-8773.
Friesen JA, Rodwell VW. The 3-hydroxy-3-methylglutaryl Coenzyme-A (HMG-CoA) Reductases. Genome Biol. 2004; 5: 248..