Antihypertensive and Cardioprotective Effects of Salvia officinalis (Sage) Leaf in N<sup>G</sup>Nitro-L-Arginine Methyl Ester (L-NAME) Induced-Hypertensive Wistar Rats
. http://www.doi.org/10.26538/tjnpr/v6i12.25
Keywords:
Rats, Biochemical parameters, Sage officinalis leaf, L-NAME, HypertensionAbstract
Salvia officinalis (Common Sage) is a medicinal plant, used for the treatment of various disorders. The aim of this study was to evaluate the antihypertensive and cardioprotective effects of Nigerian grown Sage leaf extract in L-NAME induced hypertensive rats. Twenty- four male Wistar rats divided into four groups were used for the study. Group A served as control, while hypertension was induced in groups B-D by the administration of 40 mg/kg of L-NAME orally. Group A (Control), Group B; L-NAME (40 mg/kg/day), Group C; L-NAME (40 g/kg/day) plus Sage plant leaf extract (500 mg/kg/day) and Group D; L- arginine (100 mg/kg/day) plus LNAME (40 mg/kg/day). They were fed with feed and water ad-libitum for 28 days. Blood pressure was measured by tail-cuff weekly. On 29th day, serum was collected for the determination of Lactate dehydrogenase (LDH), creatinine phosphokinase-MB isoenzyme (CKMB), aspartate transaminase (AST), alanine transaminase (ALT), total cholesterol (Tchol) and triglycerides (TG), and were measured in an AS-120 Auto-Analyzer (E.LabBST, China: BA- 88A). Group C and D resulted to decrease in the systolic blood pressure in Weeks 3 and 4 at a
significant (p < 0.05) level compared to Group B. There was significant (p < 0.05) reduction in haemodynamic parameters in groups B & C in comparison with group A. There was significant (p < 0.05) decrease in the levels of CK-MB, LDH, AST, ALT, Tchol and TG in groups C & D compared with group B. Administration of Sage leaf extract with L-NAME possesses antihypertensive and cardioprotective activity in L-NAME induced hypertension in rats.
References
Mali VR, Mohan V, bodhankar SL. Antihypertensive and cardioprotective effects of the Lagenaria siceraria fruit in NGnitro-L-arginine methyl ester (L-NAME) induced hypertensive rats. Pharm Biol. 2012; 50(11):1428-1435.
Nwokocha CR, Ozolua RI, Owu DU, Nwokocha MI, Ugwu AC. Antihypertensive properties of Allium sativum (garlic) on normotensive and two kidney one clip hypertensive rats. Nig J Physiol Sci. 2011; 26(12):213-218
Badary OA, Yassin NAZ, El-Shenawy SMA, El-Moneem MA, AL-Shafeiy HM. Study of the effect of Allium porrum on hypertension induced in rats. Rev Latinoam Quim. 2013; 41(3):149-160
Pierdomenico SD, Nicola MD, Esposito A, Mascio RD, Ballone E, Lapenna D, Cuccurullo F. Prognostic value of different indices of blood pressure variability in hypertensive patients. Am J Hypertens. 2009; 22(8):842–847.
Mali VR and Bodhankar SL. Cardioprotective effect of Lagenari siceraria (LS) fruit powder in isoprenalin-induced cardiotoxicity in rats. Eur J Integ Med. 2010a; 2:143–149.
Mali VR and Bodhankar SL. Effect of Lagenaria siceraria (LS) powder on dexamethasone induced hypertension in rats. Int J Adv Pharm Sci. 2010b; 1:50-53.
Pitts S and Adams R. Emergency department hypertension and regression to the mean. Ann Emerg Med. 1998; 31(2):214–218.
Akinlua JT, Meakin R, Umar AM, Freemantle N. Current prevalence pattern of hypertension in Nigeria: A systematic review. PLoS One. 2015; 10(10):1-18.
Mokogwu ATH, Adjekuko CO, Oshilonyah UH, Ikpefan JO, Eyenubo OB, Avwioro OG. Hypoglycaemic and Hypolipidemic Effects of Alcoholic Extract of Common Sage (Salvia Officinalis) In Streptozotocin –Induced Diabetic Rabbits. Afr J Biomed Res. 2020; 25(6):243-247.
Hamidpour M, Hamidpour R, Hamidpour S, Shahlar M. Chemistry, pharmacology, and medicinal property of sage (Salvia) to prevent and cure illnesses such as obesity, diabetes, depression, dementia, lupus, autism, heart disease, and cancer. J Trad Compl Med. 2014; 4(2):82-88.
Zarei A, Vaezi G, Malekirad AA, Abdollahi M. Hypoglycemic and hypolipidemic activities of Salvia hydrangea in streptozotocin-induced diabetes in rats. Iran J Med Sci. 2015; 18:417-422.
Kanana FM, Kibet JM, Clement JM, Maina M. Hypoglycaemic effects of Sage officinalis extracts on alloxan-induced diabetic swiss albino mice. J Med Plant Res. 2020; 14(10):518-525.
Azad MAM, Al-Mahmud A, Islam MS, Gouhar AI. Common sage (Salvia officinalis) antiviral role: potential of a Unani hand sanitizer in COVID-19 (corona virus) second wave control. AJMBR. 2020; 6(4):611-617.
Devin SR, Devin KR, Tamizgran M. Sage and treatment of diseases: A mini review. Am J Biomed Sci Res. 2021; 15(1):108- 109.
OECD. Cooperation and Development. Guidance Document on the Recognition, Assessment and Use of Clinical Signs as Humane Endpoints for Experimental Animals Used in Safety Evaluation, Paris 2002: OECD Publishing.
Nguelefack TB, Mekhfib H, Dongmoc AB, Dimod T, Watchoa P , Zoheire J, Legssyerb A, Kamanyi A, Ziyyat A. Hypertensive effects of oral administration of the aqueous extract of Solanum torvum fruits in L-NAME treated rats: Evidence from in vivo and in vitro studies. J Ethnopharmacol. 2009; 124:592-599.
Hsieh NK, Wang JY, Liu JC, Wang SD, Chen HI. Nitric oxide inhibition accelerates hypertension and induces perivascular inflammation in rats. Clin Exp Pharmacol Physiol. 2004; 31:212– 218.
Vogel GH and Vogel VH. Drug Discovery and Evaluation, Pharmacological Assay. Second ed. Berlin, Heidelberg, New York: Springer-Verlag. 2008; 246-247.
Mokogwu ATH, Adjekuko CO, Oshilonyah HU, Avwioro OG. Studies of Salvia officinalis leaf extract on some biochemical parameters in rats induced with overdosed-tramadol. Afr J Biotech. 2022; 21(8):353-360
Ghorbani A and Esmaeilizadeh M. Pharmacological properties of Salvia officinalis and its components. J Compl Med. 2017; 7(4):433-440
Qnais EY, Abu-Dieyeh M, Abdulla FA, Abdalla SS. The ntinociceptive and anti-inflammatory effects of Salvia officinalis leaf aqueous and butanol extracts. Pharm Biol. 2010; 48:1149–1156.
Abad NAA, Nouri MHK, Tavakkoli F. Effect of Salvia officinalis hydroalcoholic extract on vincristine-induced neuropathy in mice. Chin J Nat Med. 2011; 9:354-358.
Pra VD, Bisol LB, Detoni S, Denti M, Grando J. Antiinflammatory activity of fractionated extracts of Salvia officinalis. J Appl Pharm Sci. 2011; 1:67-71.
Rodrigues MR, Kanazawa LK, das Neves TL. Antinociceptive and anti-inflammatory potential of extract and isolated compounds from the leaves of Salvia officinalis in mice. J Ethnopharmacol. 2012; 139:519–526.
Khayate-Nouri MH, Namvaran-Abbasabad A, Tavakkoli F. Salvia Officinalis and cisplatin effects on pentylenetetrazole induced seizure threshold print. Zahedan J Res Med Sci. 2013; 15:1–3.
Mansourabadi AM, Sadeghi HM, Razavi N, Rezvani E. Antiinflammatory and analgesic properties of salvigenin, Salvia officinalis flavonoid extracted. Adv Herb Med. 2015; 31-41.
Brankovic S, Radenkovic M, Kitic D, Veljkovic S, Ivetic V, Pavlovic D, Miladinovic B. Comparison of the hypotensive and bradycardic activity of ginko, garlic, and onion extracts. Clin Exp Hypertens. 2011; 33(2):9-95.
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