Development of Topical Formulations with Antibacterial Efficacy from Peel Extract of Mangifera indica: Emulsions and Micro-emulsions

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Pornsak Sriamornsak
Arzu Polat
Bighan Torabi
Wantanwa Krongrawa
Anne Krüger-Genge
Joachim Storsberg
Tassilo Seidler
Mont Kumpugdee-Vollrath


Because some pathogenic bacteria are becoming more resistant to known antibiotics, more research into the development of new antibacterial agents is required. The main study objective was to develop several formulations containing peel extract of Mangifera indica (mango) and evaluate their antibacterial efficacy. The macerating extraction process was used to produce the mango peel extract. Cytotoxicity of the mango peel extract was also evaluated. The emulsions and microemulsions were prepared and assessed based on their appearance, phase separation, consistency, viscosity, and conductivity. The formulations were then evaluated for antibacterial and growth inhibitory effects against gram-negative (Escherichia coli) and gram-positive (Staphylococcus aureus) bacteria. The findings revealed that no cytotoxic effects on skin keratinocyte cells were identified. Most formulations were physico-chemically acceptable. Only S. aureus bacteria were inhibited by emulsions and microemulsions containing mango peel extract. 

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Sriamornsak, P., Polat, A., Torabi, B., Krongrawa, W., Krüger-Genge, A., Storsberg, J., Seidler, T., & Kumpugdee-Vollrath, M. (2022). Development of Topical Formulations with Antibacterial Efficacy from Peel Extract of Mangifera indica: Emulsions and Micro-emulsions: Tropical Journal of Natural Product Research (TJNPR), 6(12), 2014–2019. Retrieved from
Author Biography

Pornsak Sriamornsak, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand

Academy of Science, The Royal Society of Thailand, Bangkok 10300, Thailand


Iyer CPA and Degani C. Classical breeding and genetics. In: Litz RE (Ed.). The mango: botany, production and uses. New York: CAB International; 1997; 49-68.

Viruel MA, Escribano P, Barbieri M, Ferri M, Hormaza JI. Fingerprinting, embryo type and geographic differentiation in mango (Mangifera indica L., Anacardiaceae) with microsatellites. Mol Breed 2005; 15:383-393.

Yamanaka S, Hosaka F, Matsumura M, Onoue-Makishi Y, Nashima K, Urasaki N, Ogata T, Shoda M, Yamamoto T. Genetic diversity and relatedness of mango cultivars assessed by SSR markers. Breed Sci 2019; 69(2):332-344.

Reddy LV, Reddy OVS, Wee YJ. Production of ethanol from mango (Mangifera indica L.) peel by Saccharomyces cerevisiae CFTRI101. Afr J Biotechnol. 2011; 10(20):4183-4189.

Ajila CM, Bhat SG, Prasada Rao UJS. Valuable components of raw and ripe peels from two Indian mango varieties. Food Chem 2007; 102:1006-1011.

Cheok CY, Mohd Adzahan N, Abdul Rahman R, Zainal Abedin NH, Hussain N, Sulaiman R, Chong GH. Current trends of tropical fruit waste utilization. Crit Rev Food Sci Nutr. 2018; 58:335-361.

Chaiwarit T, Kantrong N, Sommano SR, Rachtanapun P, Junmahasathien T, Kumpugdee-Vollrath M, Jantrawut P. Extraction of tropical fruit peels and development of HPMC film containing the extracts as an active antibacterial packaging material. Molecules. 2021; 26:2265.

Thambi PA, John S, Lydia E, Iyer P, Monica SJ. Antimicrobial efficacy of mango peel powder and formulation of recipes using mango peel powder (Mangifera indica L.). Int J Home Sci. 2016; 2(2):155-161.

Smith EW, Surber C, Tassopoulos T, Maibach HI. Topical dermatological vehicles: a holistic approach. In: Bronaugh RL, Maibach HI (Eds.). Percutaneous absorption: drugs, cosmetics, mechanisms, methodology. New York: Marcel Dekker; 1999; 779-787.

Jayalaxmi B, Vijayalakshmi D, Kapale M. Extraction of Total Polyphenols and Dietary Fiber from Mango Peel - As Potential Sources of Natural Phytonutrients. Int J Curr Microbiol App Sci. 2018; 7(5):1196-1205.

Karunanithi B, Bogeshwaran K, Tripuraneni M, Krishna RS. Extraction of Mango Seed Oil from Mango Kernel. Int J Eng Res and Develop. 2015; 11(11):32-41.

Palmeira SMV, Gois LM, Souza LD. Extraction of phenolic compounds from mango peels. Latin American Appld Res 2012; 42:77-81.

Kaur B, Panesar PS, Thakur A. Extraction and evaluation of structural and physicochemical properties of dietary fiber concentrate from mango peels by using green approach. Biomass Conversion and Biorefinery 2021;

Method for extracting pigment from mango peel. 2013; CN104710820A

Pengon S, Limmatvapirat C, Sriamornsak P, Limmatvapirat S. Factors affecting formation of emulsions containing soybean oil. Adv Mater Res. 2013; 747:725-728.

Piriyaprasarth S, Juttulapa M, Sriamornsak P. Stability of rice bran oil-in-water emulsions stabilized by pectin-zein complexes: Effect of composition and order of mixing. Food Hydrocoll. 2016; 61:589-598.

Mikula RJ. Emulsion characterization. In: Schramm LL (Ed.). Emulsions. Washington, DC: American Chemical Society. 1992; 79-129 p.

Paul B and Moulik S. Uses and applications of microemulsions. Curr Sci 2001; 80(8):990-1001.

Buranatrakul P, Sornchaithawatwong C, Thongnopkun T, Phumchalao K, Naksrichum P, Phrompittayarat W. Formulation and stability of prasaplai microemulsions. Sci Eng Health Stud. 2021; 15:21050004.

Soradech S, Kusolkumbot P, Thubthimthed S. Development and characterization of microemulsions containing Tiliacora triandra Diels as an active ingredient for antioxidant and melanogenesis stimulating activities. J Appl Pharm Sci. 2018; 8:46-54.

Awad El-Gied AA, Abdelkareem AM, Hamedelnie EI. Investigation of cream and ointment on antimicrobial activity of Mangifera indica extract. J Adv Pharm Technol Res. 2015; 6(2):53-57.

Chirayath RB, Aparna V, Jayakumar R, Biswas R, Vijayachandran LS. Development of Mangifera indica leaf extract incorporated carbopol hydrogel and its antibacterial efficacy against Staphylococcus aureus. Colloid Surf B 2019; 178:377-384.

Kyadarkunte A, Patole M, Pokharkar V. In vitro cytotoxicity and phototoxicity assessment of acylglutamate surfactants using a human keratinocyte cell line. Cosmetics. 2014; 1(3):159-170.

Ansari J, Kushwaha SP, Ansari VA, Singh K, Hasan SM. Agar well diffusion: A prominent method for In vitro screening of antimicrobials. Int J Bot Stud. 2021; 6(5):836-839.

Burapapadh K, Takeuchi H, Sriamornsak P. Novel pectin-based nanoparticles prepared from nanoemulsion templates for improving in vitro dissolution and in vivo absorption of poorly water-soluble drug. Eur J Pharm Biopharm. 2012; 82(2):250-261.

Lauricella M, Lo Galbo V, Cernigliaro C, Maggio A, Palumbo Piccionello A, Calvaruso G, Carlisi D, Emanuele S, Giuliano M, D'Anneo A. The anti-cancer effect of Mangifera indica L. peel extract is associated to γH2AX-mediated apoptosis in colon cancer cells. Antioxidants. 2019; 8(10):422.

Zhang J, Ge D, Wang X, Wang W, Cui D, Yuan G, Wang K, Zhang W. Influence of surfactant and weak-alkali concentrations on the stability of O/W emulsion in an alkali-surfactant–polymer compound system. ACS Omega. 2021; 6(7):5001-5008.

Tuncay TS and Algın YE. Preparation and characterization of herbal emulsion formulations. Marmara Pharm J. 2017; 21(4):756-761.

Yin F, Liu Q, Zhang B, Zhang X, He J, Xie J, Hu Z, Sun R. Microemulsion preparation of Waltheria indica extracts and preliminary antifungal mechanism exploration. Ind Crops Prod 2021; 172:114000.

Taylor P. Ostwald ripening in emulsions: estimation of solution thermodynamics of the disperse phase. Adv Colloid Interface Sci. 2003; 106(1-3):261-285.

Bumajdad A and Eastoe J. Conductivity of water-in-oil microemulsions stabilized by mixed surfactants. J Colloid Interface Sci 2004; 274:268-276.

Handayani DS, Pranoto, Saputra DA, Marliyanaal SD. Antibacterial activity of polyeugenol against Staphylococcus aureus and Escherichia coli. IOP Conf Ser Mater Sci Eng 2019; 578:012061.

Xie W, Xu P, Wang W, Liu Q. Preparation and antibacterial activity of a water-soluble chitosan derivative. Carbohydr Polym. 2002; 50(1):35-40.

Okareh OT, Alaiya MA, Odeniyi MA. Formulation of antiseptic ointments from Mangifera indica kernel, leaf and Psidium guajava leaf extracts. Trop J Nat Prod Res. 2019; 3(10):307-313.