Black Cumin Inhibits Pro-Atherogenic Changes and Reduces Aortic Intima Media Thickness in Rats with Sub-Chronical Cigarette Smoke Exposure http://www.doi.org/10.26538/tjnpr/v6i12.17
Article Sidebar
Main Article Content
Abstract
The study aims to explore the association between black cumin extract supplementation to endothelial dysfunction markers and aortic Intima Media Thickness (IMT) in rats with subchronical cigarette smoke exposure. Fifty Wistar rats (Rattus norvegicus) were classified into five kind of groups: negative control group (NC); positive control group (PC), which was shown up to 40 cigarettes/day for about 4 weeks; and three groups shown up to cigarette smoke and it has administration of extract of black cumin ethanolic for about four weeks but in different amount of doses: 0.3 g/kg/day (T1); 0.6 g/kg/day (T2); and 1.2 g/kg/day (T3). After getting intervented, aorta was examined to measure the level of vascular cell adhesion molecule-1 (VCAM-1), endothelial nitric oxide synthase (eNOS), and also IMT. The dominant active substances of black cumin are linoleic acid (71.31%), palmitate acid (15.86%), stearate (4.69%), octadicadienal (3.8%), beta monolinolein (1.8%), and thymoquinone (0.28%). eNOS level significantly increased in T1 compared to PC with p=0.001. A significant descending in VCAM- 1 was found in T2 (p=0.014) and T3 (p=0.044) compared to PC. Aortic IMT decreased significantly in T1 (p=0.009), T2 (p=0.000), and also T3 (p=0.043) than the PC. Increased eNOS, decreased VCAM-1 and IMT following black cumin extract supplementation showed that black cumin may prevent endothelial dysfunction.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
References
Widmer RJ and Lerman A. Endothelial dysfunction and cardiovascular disease. Glob Cardiol Sci Pract. 2014; 2014(3):291-308.
Daiber A, Steven S, Weber A, Shuvaev VV, Muzykantov VR, Laher I, Li H, Lamas S, Münzel T. Targeting vascular (endothelial) dysfunction. Br J Pharmacol. 2017 Jun 1;174(12):1591–619.
Mudau M, Genis A, Lochner A, Strijdom H. Endothelial dysfunction: the early predictor of atherosclerosis. Cardiovasc J Afr. 2012; 23(4):222-31.
Shah PK. Pathogenesis of Atherosclerosis. In: Essential Cardiology: Principles and Practice. 2013; 377–86p.
Hahad O, Arnold N, Prochaska JH, Panova-Noeva M, Schulz A, Lackner KJ, Pfeiffer N, Schmidtmann I, Michal M, Beutel M, Wild PS, Keaney JF Jr, Daiber A, Münzel T. Cigarette Smoking Is Related to Endothelial Dysfunction of Resistance, but Not Conduit Arteries in the General Population-Results From the Gutenberg Health Study. Front Cardiovasc Med. 2021 19; 8:674622.
Mudau M, Genis A, Lochner A, Strijdom H. Review Article Endothelial dysfunction : the early predictor of atherosclerosis. 2012; 23(4):222–31.
Manduteanu I and Simionescu M. Vascular Disorders Review Series Inflammation in atherosclerosis : a cause or a result of vascular disorders ? Dyslipidaemia and inflammation : partake in atherosclerosis Irrespective of the initial cause susceptible areas Consecutive stages delineate ath. 2012; 16(9):1978–90.
Smiljic S. The clinical significance of endocardial endothelial dysfunction. Medicina (Kaunas). 2017; 53(5):295–302.
Barbara Messner and David Bernhard.Smoking and Cardiovascular Disease, Mechanisms of Endothelial Dysfunction and Early Atherogenesis. Arteriosclerosis, Thrombosis, and Vascular Biology. 2014; 34:509–515.
Prefontaine D, Jumarie C, Porter A. In vitro bioactivity of combustion products from 12 tobacco constituents. 2006; 44:724–38.
Incalza MA, Oria RD, Natalicchio A, Perrini S, Laviola L, Giorgino F. Oxidative Stress and Reactive Oxygen Species in Endothelial Dysfunction Associated with Cardiovascular and Metabolic Diseases. Vascul Pharmacol. 2018; 100:1-19.
Park K-H and Park WJ. Endothelial Dysfunction: Clinical Implications in Cardiovascular Disease and Therapeutic Approaches. J Korean Med Sci. 2015; 30(9):1213–25.
Ulrich F and William C. Sessa, Nitric oxide synthases: regulation and function. Euro Heart J. 2012; 33(7):829–837.
Meurer SK, Weiskirchen R. Endoglin. An ‘Accessory’ Receptor Regulating Blood Cell Development and Inflammation. Inte J Mole Sci. 2020; 21(23):9247.
Jerkic M, Rodríguez-Barbero A, Prieto M, Toporsian M, Pericacho M, Rivas-Elena JV, Obreo J, Wang A, PérezBarriocanal F, Arévalo M, Bernabéu C, Letarte M, López-Novoa JM. Reduced angiogenic responses in adult Endoglin heterozygous mice. Cardiovasc Res. 2006 Mar 1;69(4):845-54.
Rathouska J, Nemeckova I, Zemankova L, Strasky Z. Cell adhesion molecules and eNOS expression in aorta of normocholesterolemic mice with different predispositions to atherosclerosis. 2015; 30(2):241-8.
Karbach S, Wenzel P, Waisman A, Munzel T, Daiber A. eNOS uncoupling in cardiovascular diseases--the role of oxidative stress and inflammation. Curr Pharm Des. 2014; 20(22):3579–94.
Galkina E and Ley K. Vascular Adhesion Molecules in Atherosclerosis. Arteriosclerosis, Thrombosis, and Vascular Biology. 2007; 27(11):2292–2301.
Santos JCD, Cruz MS, Bortolin RH, Oliveira KM, Araújo JNG, Duarte VHR, Silva AMGD, Santos ICCD, Dantas JMO, Paiva MSMO, Rezende AA, Hirata MH, Hirata RDC, Luchessi AD, Silbiger VN. Relationship between circulating VCAM-1, ICAM- 1, E-selectin and MMP9 and the extent of coronary lesions.
Clinics (Sao Paulo). 2018; 73:e203.
Sabzghabaee AM, Dianatkhah M, Sarrafzadegan N, Asgary S, Ghannadi A. Clinical evaluation of Nigella sativa seeds for the treatment of hyperlipidemia: a randomized, placebo controlled clinical trial. Med Arch (Sarajevo, Bosnia Herzegovina). 2012; 66(3):198–200.
Ardiana M, Utami E, Pikir B, Santoso A. Preventive effect of Nigella sativa on M1/M2 ratio, reducing risk of endothelial dysfunction in cigarette smoked Wistars. F1000Research. 2021; 10:917
Al Turkmani MO, Karabet F, Mokrani L, Soukkarieh C. Chemical composition andin vitro antioxidant activities ofessential oil fromNigella sativa L. seeds cultivated in Syria. Int J ChemTech Res. 2015; 8(10).
Jaldin RG, Castardelli É, Perobelli JE, Yoshida WB, de Castro Rodrigues A, Sequeira JL, Paiva SA. Morphologic and biomechanical changes of thoracic and abdominal aorta in a rat model of cigarette smoke exposure. Ann Vasc Surg. 2013 Aug;27(6):791-800.
Ali SS, Ayuob NN, Al Ansary AK, Soluman ER. Antioxidants protect against increased risk of atherosclerosis induced by exposure to cigarette smoke: Histological and biochemical study. Saudi J Biol Sci. 2012; 19(3):291–301.
Ghahramanloo KH, Kamalidehghan B, Akbari Javar H, Teguh Widodo R, Majidzadeh K, Noordin MI. Comparative analysis of essential oil composition of iranian and indian nigella sativa L. Extracted using supercritical fluid extraction and solvent extraction. Drug Des Devel Ther. 2017; 11:2221–6.
Yang GH, Li YC, Wang ZQ, Liu B, Ye W, Ni L, Zeng R, Miao SY, Wang LF, Liu CW. Protective effect of melatonin on cigarette smoke-induced restenosis in rat carotid arteries after balloon injury. J Pineal Res. 2014 Nov;57(4):451-8.
Pott GB, Ph D, Tsurudome M, Bui J, Marc L. VCAM-1 Mediates Cigarette Smoke Extract Enhancement of Monocyte Adhesion to Human Carotid Vascular Endothelial Cells. Med Res Arch. 2017; 5(7):1–15.
He Z, Chen Y, Hou C, He W, Chen P. Cigarette smoke extract changes expression of endothelial nitric oxide synthase (eNOS) and p16(INK4a) and is related to endothelial progenitor cell dysfunction. Med Sci Monit. 2017; 23:3224–31.
Pini A, Boccalini G, Baccari MC, Becatti M, Garella R, Fiorillo C, Calosi L, Bani D, Nistri S. Protection from cigarette smokeinduced vascular injury by recombinant human relaxin-2 (serelaxin). J Cell Mol Med. 2016 May;20(5):891-902.
Kaur R, Kaur M, Singh J. Endothelial dysfunction and platelet hyperactivity in type 2 diabetes mellitus: Molecular insights and therapeutic strategies. Cardiovasc Diabetol. 2018; 17(1):1–17.
Petersen EJ, Miyoshi T, Yuan Z, Hirohata S, Li JZ, Shi W, Angle JF. siRNA silencing reveals role of vascular cell adhesion molecule-1 in vascular smooth muscle cell migration. Atherosclerosis. 2008 Jun;198(2):301-6.
Toma L, Sanda GM, Deleanu M, Stancu CS, Sima A V. Glycated LDL increase VCAM-1 expression and secretion in endothelial cells and promote monocyte adhesion through mechanisms involving endoplasmic reticulum stress. Mol Cell Biochem. 2016; 417(1–2):169–79.
Mu W, Chen M, Gong Z, Zheng F, Xing Q. Expression of vascular cell adhesion molecule-1 in the aortic tissues of atherosclerotic patients and the associated clinical implications. Exp Ther Med. 2015; 10(2):423–8.
Zhou J, Li YS, Nguyen P, Wang KC, Weiss A, Kuo YC, Chiu JJ, Shyy JY, Chien S. Regulation of vascular smooth muscle cell turnover by endothelial cell-secreted microRNA-126: role of shear stress. Circ Res. 2013 Jun 21;113(1):40-51.
Jaldin RG, Castardelli É, Perobelli JE, Yoshida WB, de Castro Rodrigues A, Sequeira JL, Paiva SA. Morphologic and biomechanical changes of thoracic and abdominal aorta in a rat model of cigarette smoke exposure. Ann Vasc Surg. 2013 Aug;27(6):791-800.
Ali SS, Ayuob NN, Al Ansary AK, Soluman ER. Antioxidants protect against increased risk of atherosclerosis induced by exposure to cigarette smoke: Histological and biochemical study. Saudi J Biol Sci. 2012; 19(3):291–301.
Harrington J, Peña AS, Gent R, Hirte C, Couper J. Aortic Intima Media Thickness is an Early Marker of Atherosclerosis in Children with Type 1 Diabetes Mellitus. J Pediatr. 2010; 156(2):237–41.
Mohammed E, Hashem K, Rheim M. Biochemical study on the impact of Nigella sativa and virgin olive oils on cadmiuminduced nephrotoxicity and neurotoxicity in rats. J Investig Biochem. 2014; 3(2):71.
Sahak MKA, Kabir N, Abbas G, Draman S, Hashim NH, Hasan Adli DS. The role of Nigella sativa and its active constituents in learning and memory. Vol. 2016, Evidence-based Complementary and Alternative Medicine. Hindawi Limited; Volume 2016, Article ID 6075679, 6 pages.
Farah N, Fauzi A, Hidayah N, Bakar A, Mohamad N, Hadzrullathfi S, Omar S, Ismail M Z, Thoarlim A , Mustaf N S. Nigella sativa Effects on Neurotransmitter Systems : Potential Treatment for Drug Tolerance and Dependence. 2018; 7(1):196– 200.
Münzel T, Gori T, Bruno RM, Taddei S. Is oxidative stress a therapeutic target in cardiovascular disease? Eur Heart J. 2010; 31(22):2741–8.
Abdelghany TM, Ismail RS, Mansoor FA, Zweier JL, Lowe F, Zweier JL. Cigarette smoke constituents cause endothelial nitric oxide synthase dysfunction and uncoupling due to depletion of tetrahydrobiopterin with degradation of GTP cyclohydrolase. Nitric Oxide. 2018; 76:113-121.
El-Agamy DS and Nader MA. Attenuation of oxidative stressinduced vascular endothelial dysfunction by thymoquinone. Exp Biol Med. 2012; 237(9):1032–8.
Rahma H, Indrawan IWA, Nooryanto M, Keman K, Rahajeng. Effect of a black cumin ( Nigella sativa ) ethanol extract on placental angiotensin II type 1-receptor autoantibody ( AT1-AA ) serum levels and endothelin-1 ( ET-1 ) expression in a preeclampsia mouse model. J Taibah Univ Med Sci. 2017;
(July):1–6.
Mahmoud YK and Abdelrazek HMA. Biomedicine & Pharmacotherapy Cancer : Thymoquinone antioxidant / prooxidant e ff ect as potential anticancer remedy. 2019; 115.
Abbasnezhad A, Niazmand S, Rezaee SA, Soukhtanloo M, Mosallanejad R. Nigella sativa L. seed regulated eNOS, VCAM- 1 and LOX-1 genes expression and improved vasoreactivity in aorta of diabetic rat. J Ethnopharmacol. 2019; 228:142-147.
Khan BV, Harrison DG, Olbrych MT, Alexander RW, Medford RM. Nitric oxide regulates vascular cell adhesion molecule 1 gene expression and redox-sensitive transcriptional events in human vascular endothelial cells. Proc Natl Acad Sci USA. 1996; 93(17):9114–9.
De Caterina R, Libby P, Peng HB, Thannickal VJ, Rajavashisth TB, Gimbrone MA Jr, Shin WS, Liao JK. Nitric oxide decreases cytokine-induced endothelial activation. Nitric oxide selectively reduces endothelial expression of adhesion molecules and proinflammatory cytokines. J Clin Invest. 1995 Jul;96(1):60-8.
Umar S, Hedaya O, Singh AK, Ahmed S. Thymoquinone inhibits TNF-α-induced inflammation and cell adhesion in rheumatoid arthritis synovial fibroblasts by ASK1 regulation. Toxicol Appl Pharmacol. 2015; 1–7.
Al-Naqeep G, Al-Zubairi AS, Ismail M, Amom ZH, Esa NM. Antiatherogenic potential of nigella sativa seeds and oil in dietinduced hypercholesterolemia in rabbits. Evidence-based Complement Altern Med. 2011; 2011:213628.
Cüce G, Sözen ME, Çetinkaya S, Canbaz HT, Seflek H, Kalkan S. Effects of Nigella sativa L . seed oil on intima – media thickness and Bax and Caspase 3 expression in diabetic rat aorta. 2016; (10):460–6.
Luengas C, Rueda-clausen CF, Patricio L, Oubi P, Cachofeiro V, Lahera V. Inflammation but Not Endothelial Dysfunction Is Associated with the Severity of Coronary Artery Disease in Dyslipidemic Subjects. 2009; 2009:469169