Phytochemical, Antioxidant Analysis and In Vitro Xanthine Oxidase Inhibitory Activity of Kaempferia parviflora and Kaempferia galanga
http://www.doi.org/10.26538/tjnpr/v6i12.14
Keywords:
Kaempferia galanga, Kaempferia parviflora, Xanthine oxidase, Antioxidant, PhytochemicalsAbstract
Kaempferia galanga and Kaempferia parviflora are employed in the functional food and nutraceutical industries and have many health benefits. Both plants contain bioactive substances with anti-inflammatory and alternative therapeutic potential for gout-related inflammation. The purpose of this was to compare the phytochemical, antioxidant, and xanthine oxidase inhibiting activities of rhizome extracts from K.galanga and K.parviflora using in vitro assays. The test tube approach was employed to perform phytochemical screening analysis. The spectrophotometric method using 1,1-diphenyl-2-picryl-hydrazyl was employed to measure the antioxidant activity. UV spectrophotometry was employed to determine the in vitro xanthine oxidase inhibitory activity with Allopurinol as a positive control. The results showed that tannins, alkaloids, flavonoids, and polyphenols were present as phytochemical constituents. Based on the Folin-Ciocalteu technique, the level of phenolic compounds in K.parviflora and K.galanga was 52.33 and 50.35 mgGAE/100g, respectively. K.parviflora contains 144.2 mg total flavonoid content and K.galanga has 20.98 mgQE/100 g. In K.parviflora, free radical scavenging activity was measured using the IC50 (DPPH method), with a value of 547.202 ± 3.88 µg/mL, and K.galanga with a value of 626.308 ± 5.06 µg/mL. K.parviflora also has the highest inhibitory effect with an IC50 value of 29.69 ± 3.27 µg/mL while K.galanga has 139.92 ± 0.51 µg/mL based on the xanthine oxidase inhibitory activity using in vitro assay. The findings
helped to shed light on some of this species' phytochemical traits and indicate that both plants are worth future investigation for an anti-gout in vivo assay using an animal test.
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